Collagen Hydrogels Loaded with Silver Nanoparticles and Cannabis Sativa Oil

Abstract

Wounds represent a major healthcare problem especially in hospital-associated infections where multi-drug resistant strains are often involved. Nowadays, biomaterials with therapeutic molecules play an active role in wound healing and infection prevention. In this work, the development of collagen hydrogels loaded with silver nanoparticles and Cannabis sativa oil extract is described. The presence of the silver nanoparticles gives interesting feature to the biomaterial such as improved mechanical properties or resistance to collagenase degradation but most important is the long-lasting antimicrobial effect. Cannabis sativa oil, which is known for its anti-inflammatory and analgesic effects, possesses antioxidant activity and successfully improved the biocompatibility and also enhances the antimicrobial activity of the nanocomposite. Altogether, these results suggest that this novel nanocomposite biomaterial is a promising alternative to common treatments of wound infections and wound healing.

Ag Nanoparticles for Biomedical Applications—Synthesis and Characterization—A Review

“…Since the size of nanoparticles and their antibacterial effect are dependent properties [ 22 ], this method of encapsulation is a promising result in tissue regeneration applications [ 21 ]. Another strategy in composites is based on the development of polymeric hydrogels loaded with silver nanoparticles [ 23 , 24 , 25 ]. The variety of infections produced in recent years by bacteria led to the research of alternative solutions that are easily implemented by patients.…”

Nanoparticle-Containing Wound Dressing: Antimicrobial and Healing Effects

“…Antezana et al [ 23 ] studied the antibacterial activity and biocompatibility of hydrogels based on collagen and spherical AgNPs ranging from 10 to 15 nm. The growth inhibiting effect of the hydrogels on the S. aureus and P. aeruginosa was established, as evidenced by the presence of the zones of inhibition vs. the nanoparticle-free hydrogel ( Figure 1 ).…”

Controlled Bioactive Delivery Using Degradable Electroactive Polymers

“…Briefly, the cell culture medium was removed and a solution of MTT in PBS 1× (5 mg/mL) was added to the cells and incubated at 37 °C for 4 h. Then, the MTT solution was discarded, films were washed with PBS 1X and absolute ethanol was added. Finally, the absorbance of the purple solution was measured at 570 nm and results were expressed as mean ± SD from triplicate experiments. − …”