Application of Raman Spectroscopy for Analysis of Carbon Nanotube Distribution in Living Cells

Golubewa, Lena; Шуба, М. В.; Vasilieu, M. V.; Kulahava, Tatsiana

doi:10.1007/s10812-019-00768-7

Cited by 9 publications

(9 citation statements)

References 19 publications

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“…The accumulation rate is length-dependent with fast elimination of SWCNTs in 8 h via exocytosis [25]. We have already shown [11,26] that in a non-phagocytic glioma cells, SWCNT accumulation lasts for more extended periods (>18 h), reaching saturation. In the present study, cells exposed to 5 μg ml −1 of SWCNT-DNA complexes, cells exposed to the DNA, and cells in control Interaction of CNTs with living cells and their accumulation inside has been widely discussed in terms of initiating autophagic cell death [27,28], accompanied by an inflammatory response and oxidative stress [29].…”

Section: Accumulation Dynamics Of Swcnt-dna Complexes Inside C6 Gliom...mentioning

confidence: 93%

“…Single-walled (SW) and multi-walled (MW) carbon nanotubes (CNTs) exhibit unique physical, chemical, and biological properties [6][7][8], which allow one to use them in a capacity of theranostic agents. Nano-needle shape with a hollow core and high aspect ratio [9] makes it possible to use SWCNTs and MWCNTs for drug delivery, well-developed functionalization allows one to employ them to target the tumor [10], while their intense Raman spectra can be employed to visualize tumor cells [11]. That is, CNTs are capable to provide (i) a source of imaging signal, (ii) a therapeutic drug, (iii) a carrier of a therapeutic drug, and (iv) targeting ligand for selective accumulation in the tumor [12].…”

Section: Introductionmentioning

confidence: 99%

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Rapid and delayed effects of single-walled carbon nanotubes in glioma cells

Golubewa¹,

Kulahava²,

Timoshchenko³

et al. 2021

Nanotechnology

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Single-walled carbon nanotubes (SWCNTs) demonstrate a strong potential as an optically activated theranostic nano-agent. However, using SWCNTs in theranostics still requires revealing mechanisms of the SWCNT-mediated effects on cellular functions. Even though rapid and delayed cellular responses can differ significantly and may lead to undesirable consequences, understanding of these mechanisms is still incomplete. We demonstrate that introducing short (150-250 nm) SWCNTs into C6 rat glioma cells leads to SWCNT-driven effects that show pronounced time dependence. Accumulation of SWCNTs is carried out due to endocytosis with modification of the actin cytoskeleton but not accompanied with autophagy. Its initial stage launches a rapid cellular response via significantly heightened mitochondrial membrane potential and superoxide anion

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Section: Accumulation Dynamics Of Swcnt-dna Complexes Inside C6 Gliom...mentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Rapid and delayed effects of single-walled carbon nanotubes in glioma cells

Golubewa¹,

Kulahava²,

Timoshchenko³

et al. 2021

Nanotechnology

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“…The resulting SWCNT-DNA suspension was further used for biological applications. SWCNT-DNA (SWCNTs) concentration was determined via ultraviolet-visible spectroscopy (RV2201 spectrophotometer, ZAO SOLAR, Belarus) 44 . SWCNTs in suspension were stable for up to 4 months.…”

Section: Methodsmentioning

confidence: 99%

Single-walled carbon nanotubes as a photo-thermo-acoustic cancer theranostic agent: theory and proof of the concept experiment

Golubewa

Timoshchenko

Romanov

et al. 2020

Sci Rep

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Theranostics is the emerging field of medicine that uniquely combines diagnostic techniques and active agents to diagnose and treat medical conditions simultaneously or sequentially. Finding a theranostic agent capable to cure the affected cells and being safe for the healthy ones is the key for successful treatment. Here, we demonstrate that agglomerated single-walled carbon nanotubes (SWCNTs) are promising theranostic agent that enables photo-activated ‘cold’ destruction of the cancer cells keeping their environment alive. The absorption of picosecond pulses by SWCNT agglomerates results in the mechanical (due to photoacoustic effect) rather than photothermal cancer cell destruction, which was visualized by micro-Raman and ultrafast near-infrared CARS. The developed theoretical model allows us to distinguish photothermal, photoacoustic, and photothermoacoustic regimes of the cancer cell destruction, and also to optimize SWCNT-based theranostics recipe.

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“…The methods for evaluating biokinetics to date include preparing and observing tissue sections, [111,112] attaching contrast materials to surfaces for labeling, [113][114][115] carbon isotopes, [116][117][118] and Raman's assay. [119][120][121][122][123] However, such problems as quantitative limitations in preparing and observing tissue sections, changes in the properties of nanocarbons, and the possibility of contrast material detachment from surfaces exist. Nanocarbons made from carbon isotopes are labor-intensive and technically demanding, and although Raman analyses can detect some nanocarbons, they are not applicable for many others.…”

Section: Biokinetic Evaluationmentioning

confidence: 99%

Future Prospects for Clinical Applications of Nanocarbons Focusing on Carbon Nanotubes

et al. 2022

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Over the past 15 years, numerous studies have been conducted on the use of nanocarbons as biomaterials towards such applications as drug delivery systems, cancer therapy, and regenerative medicine. However, the clinical use of nanocarbons remains elusive, primarily due to short‐ and long‐term safety concerns. It is essential that the biosafety of each therapeutic modality be demonstrated in logical and well‐conducted experiments. Accordingly, the fundamental techniques for assessing nanocarbon biomaterial safety have become more advanced. Optimal controls are being established, nanocarbon dispersal techniques are being refined, the array of biokinetic evaluation methods has increased, and carcinogenicity examinations under strict conditions have been developed. The medical implementation of nanocarbons as a biomaterial is in sight. With a particular focus on carbon nanotubes, these perspectives aim to summarize the contributions to date on nanocarbon applications and biosafety, introduce the recent achievements in evaluation techniques, and clarify the future prospects and systematic introduction of carbon nanomaterials for clinical use through practical yet sophisticated assessment methods.

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Application of Raman Spectroscopy for Analysis of Carbon Nanotube Distribution in Living Cells

Cited by 9 publications

References 19 publications

Rapid and delayed effects of single-walled carbon nanotubes in glioma cells

Rapid and delayed effects of single-walled carbon nanotubes in glioma cells

Single-walled carbon nanotubes as a photo-thermo-acoustic cancer theranostic agent: theory and proof of the concept experiment

Future Prospects for Clinical Applications of Nanocarbons Focusing on Carbon Nanotubes

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