Application of purified bone morphogenetic protein (BMP) in cranio-maxillo-facial surgery

Sailer, Hermann F.; Kolb, E.

doi:10.1016/s1010-5182(05)80289-6

Cited by 68 publications

(28 citation statements)

References 17 publications

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“…Clinical studies conducted on humans using this xenogenic BMP also yielded very positive results (9,10). It should be noted that the use of BMP extracted from bovine bone in the treatment of human perennial bone defects is based on the chemical homology, demonstrated by DNA cloning, between human and bovine BMPs (9,10,13).…”

Section: Discussionmentioning

confidence: 99%

Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite

et al. 2004

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The ability of a pool of bovine bone morphogenetic proteins bound to synthetic microgranular hydroxyapatite (BMPb-HA) to stimulate bone repair was determined in rat critical size defects. An 8-mm diameter defect was created in the calvaria of 25 rats. In 15 rats, the defects were filled with BMPb-HA homogenized with blood (experimental group), and in 10 rats the defects were filled only with blood clots (control). The calvariae of experimental rats were collected 1, 3 and 6 months after surgery and of the control rats at the end of surgery and 6 months thereafter. The morphometric results obtained in the radiographs showed an absence of new bone formation at 1 and 3 months post-surgery and, histologically, the defects were filled with fibrous connective tissue and numerous foci of a foreign body-type granulomatous reaction around hydroxyapatite agglomerates. At the end of 6 months, the number and size of the granulomatous foci decreased and the area of the defects was reduced by 22% compared to the 0-hour control due to the formation of new bone at their borders, although the mean area was similar to the 6-month control. We conclude that the use of BMPb-HA in the treatment of critical size bone defects of the rat skull leads to the formation of a foreign body-type granulomatous reaction that markedly inhibits new bone formation, suggesting that synthetic microgranular hydroxyapatite does not represent a good carrier for BMPinduced bone formation.

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Section: Discussionmentioning

confidence: 99%

Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite

et al. 2004

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“…7 However, the most potent inducers of ectopic bone formation around titanium implants described to date are members of the bone morphogenetic protein (BMP) family of protein growth factors. 8,9 The osteogenic capacity of many BMPs has been proven by implanting individual recombinant BMPs in a rat ectopic assay and using stringent criteria. BMP 2, 4 through 7, and 9 have been shown to be bone inductive molecules.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Mineralization Studies with Substrate-immobilized Bone Morphogenetic Protein Peptides

Kirkwood¹,

Rheude²,

Kim³

et al. 2003

Journal of Oral Implantology

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Understanding the factors that control osteoblastic behavior is centrally important in establishment of successful osseointegration. Pharmacogenetic control of the osteoblast to increase the mineral content around dental implants may offer a unique advantage to clinicians in improving osseointegration success and decreasing time before mechanical loading. This in vitro pilot study has screened for bioactive peptides derived from bone morphogenetic protein 7 (BMP-7) (also called osteogenic protein 1 [OP-1]). Thirteen overlapping peptides of BMP-7 were synthesized and covalently coupled to glass coverslip substrates using silane chemistry. The rate and relative amount of mineralization were compared by von Kossa analysis using primary rat calvarial osteoblastic cell populations during a 7-to 21-day period. In addition, bone sialoprotein (BSP) and osteocalcin (OC) gene expression was measured from osteoblastic cells grown on peptide-immobilized glass coverslips by reverse transcriptase-polymerase chain reaction. Initial results from mineralization studies suggested the BMP-7-derived peptides were able to support mineralization to varying degrees with enhanced peptide-induced mineralization from the C-and N-termini of the BMP-7 molecule. Analysis of these peptide regions indicated that these peptides comprised the finger 1 and 2 domains of OP-1, which contribute toward ligand-receptor interaction. Further analysis of gene expression from select peptide-immobilized substrates indicated that peptides from the C-terminus of BMP-7 were capable of supporting BSP and OC messenger RNA expression. These studies indicate that BMP-7 peptides covalently bound to solid substrates may provide the biological basis to immobilize peptides to titanium implants to induce osteoblastic differentiation and mineralization in a more predictable fashion.

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“…Some investigators reported that local administration of BMP-2 induces ectopic bone formation and improves the healing of fractures (Sailer and Kolb, 1994;Takahashi, 2000;Murata et al, 2000). BMP-4 expression has also been demonstrated during fracture healing (Yaoita et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical observations of cellular differentiation and proliferation in endochondral bone formation from grafted periosteum:

Ueno

Kagawa

Kanou

et al. 2003

Journal of Cranio-Maxillofacial Surgery

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Application of purified bone morphogenetic protein (BMP) in cranio-maxillo-facial surgery

Cited by 68 publications

References 17 publications

Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite

Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite

In Vitro Mineralization Studies with Substrate-immobilized Bone Morphogenetic Protein Peptides

Immunohistochemical observations of cellular differentiation and proliferation in endochondral bone formation from grafted periosteum:

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