Objectives. Endoscopic tympanoplasty (ET) provides minimally invasive transcanal access to the middle ear and improves middle ear visibility for the treatment of tympanic membrane (TM) perforation. However, literature on surgical outcomes for large TM perforations is lacking and limited to small series. This study aimed to evaluate the clinical benefits of ET for large TM perforations.Methods. This retrospective cohort study was conducted in nine tertiary referral hospitals in South Korea where 252 patients who underwent ET as a primary surgery from September 2019 to August 2021 were included. Outcome measures included graft success rate and pre- and postoperative audiometric data.Results. Of 239 patients, graft success rate of ET for large or subtotal perforations was 86.2% (206 patients), while graft failure rate was 13.8% (33 patients). The graft failure rate directly correlated with the use of surgical techniques, including overlay and medial and lateral underlay tympanoplasty (P=0.027). Sex, laterality, etiology, site and size of perforation, operation time, and graft materials did not vary significantly between the graft success and failure groups (P > 0.05). The mean air-bone gap improved significantly (graft success group; 10.0