Application of platelet-rich plasma in the in vitro production of bovine embryos

Ramos-Deus, Pamela; Nascimento, Pábola Santos; Vieira, Joane Isis Travassos; Chaves, Maiana Silva; Albuquerque, Karoline Antunes de; Ferreira-Silva, José Carlos; Grázia, João Grabriel Viana; Filho, A. S. Santos; Batista, André Mariano; Teixeira, Valéria Wanderley; Oliveira, Marcos Antônio Lemos

doi:10.1007/s11250-020-02307-5

Cited by 9 publications

(2 citation statements)

References 47 publications

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“…A few studies described the effects of PRP on in vitro embryo production (Lange‐Consiglio et al, 2015; Ramos‐Deus et al, 2020). In the study of Lange‐Consiglio et al (2015), two different percentages of PRP were used (5% and 10%), as a partial or complete replacement of foetal calf serum.…”

Section: The Use Of Prp To Improve In Vitro Embryo Production and Imp...mentioning

confidence: 99%

“…On the contrary, the lower blastocyst rate obtained in the medium with 10% PRP when compared to control might be caused by the excess of growth factors that could have an inhibitory effect on the embryo development (Lange‐Consiglio et al, 2015). Another study reported that the addition of PRP into the oocyte maturation medium is not beneficial (Ramos‐Deus et al, 2020). These promising results could attract further studies to evaluate the PRP effect on follicular recruitment, oocyte competence, in vitro fertilization and embryo development in animals.…”

Section: The Use Of Prp To Improve In Vitro Embryo Production and Imp...mentioning

confidence: 99%

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Platelet‐rich plasma in animal reproductive medicine: Prospective and applications

Silviu‐Ionuț

Ibănescu²,

Alina

et al. 2022

Reprod Domestic Animals

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Platelet‐rich plasma (PRP) is the fraction of the plasma enriched with a platelet level above baseline, and it plays an essential role in tissue regeneration. The attention to PRP as unconventional therapy has increased during the past two decades. In animals, applications of PRP showed various degrees of success in a wide range of medical applications, from musculoskeletal injuries to ovarian insufficiency. Therapeutic applications with PRP in farm animals are still scarce, but the promising results offered by several studies are expected to trigger more interest in its application by both veterinarians and farmers. In this review, we highlight some efforts made in the field of animal reproduction regarding the use of PRP to potentiate the ovarian hypofunction, treat endometritis, improve the follicular development, oocyte competence and uterine environment for embryo implantation as well as restore testicular and erectile function in male, and as a treatment for mastitis.

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Section: The Use Of Prp To Improve In Vitro Embryo Production and Imp...mentioning

confidence: 99%

Section: The Use Of Prp To Improve In Vitro Embryo Production and Imp...mentioning

confidence: 99%

Platelet‐rich plasma in animal reproductive medicine: Prospective and applications

Silviu‐Ionuț

Ibănescu²,

Alina

et al. 2022

Reprod Domestic Animals

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Use of platelet lysate for in-vitro embryo production and treatment of repeat breeding in cows

Hassaneen¹,

Rawy²,

Yamanokuchi³

et al. 2023

Theriogenology

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Lipid peroxidation in bull semen influences sperm traits and oxidative potential of Percoll®-selected sperm

Castiglioni

Siqueira

Bicudo

et al. 2021

Zygote

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Summary Although bovine embryo in vitro production (IVP) is a common assisted reproductive technology, critical points warrant further study, including sperm traits and oxidative status of sperm for in vitro fertilization (IVF). Our aim was to evaluate whether the lipid peroxidation index of commercial bull semen is influenced by sperm traits and oxidative status of sperm populations selected using Percoll® gradient. Semen straws from 48 batches from 14 Nelore bulls were thawed individually, analyzed for motility and subjected to Percoll selection. After Percoll, the lipid peroxidation index of the extender was evaluated, whereas selected sperm were analyzed for motility, acrosome and membrane integrity, mitochondrial membrane potential, chromatin resistance and oxidative potential under IVF conditions. Batches were divided retrospectively in four groups according to lipid peroxidation index. Sperm from Group 4 with the lowest index of lipid peroxidation had, after Percoll selection, greater plasma membrane integrity (81.3%; P = 0.004), higher mitochondrial potential (81.1%; P = 0.009) and lower oxidative potential (135.3 ng thiobarbituric acid reactive substances (TBARS)/ml; P = 0.026) compared with Group 1 with highest lipid peroxidation index (74.3%, 73% and 213.1 ng TBARS/ml, respectively). Furthermore, we observed negative correlations for the lipid peroxidation index with motility, membrane integrity and mitochondrial potential, and positive correlations with oxidative potential. In conclusion, oxidative stress in semen straws, as determined using lipid peroxidation in the extender, is associated with sperm traits and their oxidative potential under IVF conditions. These results provided further insights regarding the importance of preventing oxidative stress during semen handling and cryopreservation, as this could affect sperm selected for IVF. Finally, Percoll selection did not completely remove sperm with oxidative markers.

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Application of platelet-rich plasma in the in vitro production of bovine embryos

Cited by 9 publications

References 47 publications

Platelet‐rich plasma in animal reproductive medicine: Prospective and applications

Platelet‐rich plasma in animal reproductive medicine: Prospective and applications

Use of platelet lysate for in-vitro embryo production and treatment of repeat breeding in cows

Lipid peroxidation in bull semen influences sperm traits and oxidative potential of Percoll®-selected sperm

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