Application of Platelet-rich Plasma and Tricalcium Phosphate in the Treatment of Comminuted Fractures in Animals

Abstract

Aim: To assess the applicability of β-tri-calcium phosphate (TCP) and platelet-rich plasma (PRP) in the treatment of comminuted fractures in small animals. Material and Methods: The experimental study was carried out on 16 New Zealand White rabbits. After creating the bone defect and performing tibial osteotomy, TCP implants containing activated PRP were introduced into the fracture and the defect. The fracture was stabilised using external fixators or intramedullary nails. After 12 weeks, the animals were euthanised, and radiological, histological, scanning electron microscopy and peripheral quantitative computed tomography examinations were performed. The analysis also covered the results of fracture treatment in 37 small animals (cats and dogs) in which treatment with TCP containing PRP was used as an alternative to cancellous bone implantation. Results: Correct bone union was observed in the experimental groups, TCP remained visible at the site of the fracture after 12 weeks. In the clinical application in small animals, bone union was observed in over 91% of treated animals. Conclusion: β-TCP and activated PRP may be an effective method of bone union enhancement in the treatment of comminuted fractures in small animals.

Histologic, Histomorphometric, and Clinical Analysis of the Effects of Growth Factors in a Fibrin Network Used in Maxillary Sinus Augmentation

“…Platelet concentrates and beta-tricalcium phosphate (β-TCP) combination used in many medical fields. Szponder et al [17] applied β-TCP and platelet-rich plasma (PRP) combination in the treatment of comminuted fractures in New Zealand white rabbits. After creating the tibial bone defects, β-TCP implants containing PRP were put into the fracture and the defect.…”

Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen

“…Intra-articular injection of PRP also prevented cytotoxic effect of lidocaine on chondrocytes [48] Thus, intra-articular injections of PRP can be useful in angiogenesis and extracellular matrix remodeling, cell proliferation, and differentiation for osteoarthritis [12,13,15,16]. Applications of PRP in fracture repair had been shown in both dogs [49] and rabbit [48] by promoting bone healing. Interestingly, injection of PRP also promoted wound healing of soft tissue defects [50,51] as well as periodontal regeneration [52].…”

“…Several clinical studies have found that PRP injections have improved functional outcomes and reduced symptoms when compared with hyaluronic acid and placebo controls [13]. Intra-articular injection of PRP also prevented cytotoxic effect of lidocaine on chondrocytes [48] Thus, intra-articular injections of PRP can be useful in angiogenesis and extracellular matrix remodeling, cell proliferation, and differentiation for osteoarthritis [12,13,15,16]. Applications of PRP in fracture repair had been shown in both dogs [49] and rabbit [48] by promoting bone healing.…”

How much platelet-rich plasma can be soak-loaded onto beta-tricalcium phosphate? A comparison with or without a unidirectional porous structure

“…Radiographic and histological evaluations in a study on a radial defect rabbit model showed that SPTCP soak-loaded with bone marrow mesenchymal stem cells (BM-MSCs) and 200 µL of leukocyte-rich PRP (LR-PRP) promoted osteogenesis after both 4 and 8 weeks 8) . In addition, administering granulated artificial bone mixed with activated PRP promotes osteogenesis 9,10) . In a study on a tibial fracture rabbit model, administering LR-PRP mixed with SPTCP promoted osteogenesis as seen through radiological, histological, and quantitative computed tomographic images after 12 weeks 9) .…”

“…Artificial bone, which has excellent osteoconductivity and biocompatibility, is often used as a bone substitute for the reconstruction of large bones with defects and for spinal fusion 3) . However, because non-bone tissues are present in the surrounding area, various attempts have been made to promote osteoinduction in artificial bones to promote osteogenesis [4][5][6][7][8][9][10] .…”