Application of pharmacia automated FPLC System and PepRPC HR 5/5 bonded phase column for determination of dansylated polyamines

Kremmer, T.; Boldizsár, M.; Holczinger, L.

doi:10.1007/bf02280601

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…Aliquots of cells (usually 5, 30 and 50 millions for ODC, polyamine and nucleotide phosphate determinations, respective ly) from pooled cell suspensions of 5-10 mice were collected by centrifugation (5.000 rpm, 0 ®C, 10 min). ODC activity and polyamine content of tumor cells were measured as described earlier [19][20][21]. For deter mination of nucleotide phosphates, tumor cells were washed in phosphate-buffered saline (PBS.…”

Section: Methodsmentioning

confidence: 99%

In vivo Effects of <i>DL</i>-α-Difluoromethylornithine on the Polyamine and Nucleotide Phosphate Metabolism in P388/S Leukemia Cells

Kremmer¹,

Boldizsár²,

Holczinger³

1986

Pathobiology

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In vivo effects of DL-α-difluoromethylornithine (DFMO) on the metabolism of polyamines and nucleotide phosphates were monitored in P388/S leukemia cells grown intra-peritoneally in BDF₁ inbred male mice. Inhibiting the ornithine decarboxylase (ODC) activity DFMO depleted putrescine and spermidine to 30–50 and 50–60%, respectively, and increased spermine to 25–60% compared with the controls, when given as 2% solution in drinking water of the tumor-bearing animals. DFMO treatment caused a parallel 56% elevation of total nucleotide content in tumor cells with distinct and significant increase of some nucleotide phosphates. The most pronounced alterations were shown in the intracellular UTP (202%), CTP (103%), ADP (92%) and ATP (71%) concentrations. Changes in polyamine and nucleotide phosphate metabolisms were dependent on tumor progression. A possible explanation of the metabolic events induced by DFMO is discussed.

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Section: Methodsmentioning

confidence: 99%