Application of nurse culture for plant regeneration from protoplasts of Lilium japonicum thunb

Komai, Fuminori; Morohashi, Hitomi; Horita, Mitsugu

doi:10.1079/ivp2006755

Cited by 10 publications

(2 citation statements)

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“…Nurse cells may be used to promote protoplast division, particularly when the protoplasts are cultured at low density. For example, nurse cells were essential in promoting growth of protoplasts from cell suspensions of Lilium japonicum [33] and shoot regeneration from protoplast-derived tissues of banana [34]. Protoplasts or cells capable of rapid division, from the same genus, species, or cultivar can be used as a nurse culture, with protoplasts or cells from embryogenic cell suspensions being preferable to those of non-embryogenic cultures.…”

Section: Plating Density and Nurse Culturesmentioning

confidence: 99%

Plant Protoplasts: Isolation, Culture and Plant Regeneration

et al. 2010

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Section: Plating Density and Nurse Culturesmentioning

confidence: 99%

Plant Protoplasts: Isolation, Culture and Plant Regeneration

et al. 2010

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“…q-t Callus originating from bulb scale, filament, ovary and stem axis explants, respectively, observed by scanning electron microscope. Snow Queen (Tribulato et al 1997), Lilium formosanum Wallace (Nakano et al 2000), Lilium 9 formolongi 'Akasu' (Ogaki et al 2008), Oriental hybrid lily (Lilium L.) (Tribulato et al 1997;Mii et al 1994;Horita et al 2002;Komai et al 2006). However, the growth rate of stem axis suspension cultures established using NAA and TDZ combinations was rather slow.…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium-mediated transformation of embryogenic cell suspension cultures and plant regeneration in Lilium tenuifolium oriental × trumpet ‘Robina’

Quan

et al. 2014

Acta Physiol Plant

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A method has been developed for embryogenic cell suspension cultures, plant regeneration and transformation of the important ornamental lily genotype (Lilium tenuifolium oriental 9 trumpet 'Robina'). Bulb scales, filaments, ovaries and stem axis tissues were used as explants for callus induction in Murashige and Skoog (MS) medium with additions of growth regulators: picloram on its own, or in combination with 1-naphthaleneacetic acid (NAA), and thidiazuron (TDZ). The results show that the optimum medium for callus induction in bulb scale and filament tissue is MS ? picloram 1.0 mg L -1 , and for the ovary, it is MS ? picloram 1.5 mg L -1 . The stem axis had the highest rate (89.2 %) of callus induction with MS ? NAA 2.2 mg L -1 ? TDZ 0.1 mg L -1 . The suspension cultures were established with the combination of NAA and TDZ with 2-5 mm cell clusters. These took a long time compared with suspension cultures established by picloram with 1-3 mm cell clusters. In three suspension cultures induced by picloram, the best callus from the point of view of proliferation and regeneration was derived from filaments. For plant regeneration, the growth rate of suspension cultures from the stem axis was higher than from the other three suspension culture induced by picloram. Vector pCAMBIA1301 with the b-glucuronidase (GUS) gene as reporter was transformed by Agrobacterium mediation into suspension cultures initiated from filament and stem axis material. After co-cultivation, the numbers of blue spots in material from the two sources were 26.8 ± 4.3 and 24.0 ± 4.7, respectively (difference not significant). Hygromycin-resistant callus was successfully regenerated into plantlets on plant growth regulator-free MS medium. Transgenic plants were also confirmed by the GUS histochemical assay, polymerase chain reaction.

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Ornamentals

Davey

Anthony

Prentice

et al. 2008

Compendium of Transgenic Crop Plants

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The trade of ornamental plants generates substantial revenue worldwide, with molecular and somatic cell technologies providing an important and essential underpinning to conventional breeding approaches. Molecular procedures facilitate the identification of novel germplasms for incorporation into breeding programs involving sexual hybridization, while tissue culture‐based techniques are exploited to micropropagate novel germplasms and to provide the basis essential for genetic manipulation. Procedures for the genetic improvement of ornamentals involve exposure of existing genetic variation (somaclonal variation) through cell and tissue culture, somatic hybridization by the fusion of isolated protoplasts, and transformation by Agrobacterium or particle bombardment (biolistics)‐mediated gene delivery. Targets for genetic manipulation of ornamental plants include modification of stature and floral characteristics and improved tolerance to pests, diseases, and environmental stress.

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Application of nurse culture for plant regeneration from protoplasts of Lilium japonicum thunb

Cited by 10 publications

References 22 publications

Plant Protoplasts: Isolation, Culture and Plant Regeneration

Plant Protoplasts: Isolation, Culture and Plant Regeneration

Agrobacterium-mediated transformation of embryogenic cell suspension cultures and plant regeneration in Lilium tenuifolium oriental × trumpet ‘Robina’

Ornamentals

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