Application of NMR Spectroscopy to the Determination of Low Concentrations of Nonradioactive Isotopes in Liquid Media

Джимак, С. С.; Басов, А. А.; Копытов, Г. Ф.; Kashaev, D. V.; Соколов, М. Е.; Арцыбашева, О. М.; Sharapov, K. S.; Барышев, М. Г.

doi:10.1007/s11182-015-0591-9

Cited by 16 publications

(7 citation statements)

References 10 publications

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“…In the present trial, the consumption of HIDW for 2 months resulted in significantly diminished plasma levels of both water heavy isotopes as compared to the control group consuming natural tap or spring water ( Table 3 ), while the relative distribution of 2 H 1 and 18 O 16 remained similar to that of the control group ( Appendix A , Figure A1 ). Similar observations reported in previous publications [ 30 ] prompted the authors to draw the conclusion that the isotopic content of plasma depended mainly on that of the drinking water ingested. At the same time, animal experiments have shown that the drop in the plasma levels of heavy water isotopes was not as dramatic as their decrease in several organs, such as pancreas, liver, [ 30 ], muscles [ 31 ], and kidney [ 20 ].…”

Section: Discussionsupporting

confidence: 84%

Effects of Heavy Isotopes (2H1 and 18O16) Depleted Water Con-Sumption on Physical Recovery and Metabolic and Immunological Parameters of Healthy Volunteers under Regular Fitness Load

Kharaeva

Hokonova

Elmurzaeva

et al. 2021

Sports

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Water depleted of heavy isotopes, such as 2H1 and 18O16 (HIDW), has shown numerous biological/health effects in vitro, in vivo, and in epidemiological studies. Major observations were related to cell growth/differentiation, immune/nervous system responses, endurance/adaptation, mitochondrial electron transfer, energy production, glucose metabolism, etc. No human studies to confirm physiological, metabolic, and immune responses to the consumption of HIDW have been performed. A placebo-controlled study on healthy volunteers (n = 50) under fitness load who consumed 1.5 L HIDW (58 ppm 2H and 1780 ppm 18O) or normal water for 60 days was carried out. Plasma content of 2H1 and 18O16, markers of energy, lipid, and glucose metabolism, anthropometric, cardio-vascular, oxidant/antioxidant, and immunological parameters were determined. Significant decrease in plasma heavy isotopes in the group consuming HIDW was observed in concomitance with an increase in ATP, insulin, and LDH, and diminished plasma lactate. Several anthropometric and cardio-vascular parameters were improved as compared to placebo group. Lipid markers demonstrated antiatherogenic effects, while oxidant/antioxidant parameters revealed HIDW-induced hormesis. Antibacterial/antiviral immunity was remarkably higher in HIDW versus placebo group. Conclusions: HIDW consumption by humans under fitness load could be a valid approach to improve their adaptation/recovery through several mechanisms.

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Section: Discussionsupporting

confidence: 84%

Effects of Heavy Isotopes (2H1 and 18O16) Depleted Water Con-Sumption on Physical Recovery and Metabolic and Immunological Parameters of Healthy Volunteers under Regular Fitness Load

Kharaeva

Hokonova

Elmurzaeva

et al. 2021

Sports

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“…It should be pointed out that fluctuations in isotopic composition of water in a human body can have a wider range, both in connection with the consumption of not only water from surface water sources [42], but from water obtained from any other origin (artesian, glacial, mineral springs), and due to the characteristics of the food ration, for example the predominant consumption of lipid-based nutrients [43]. During study of isotopic composition of samples of bottled and packaged waters of the world, several scientific groups defined the following range of fluctuations: δ 2 H from −147‰ to 15‰ and δ 18 O from −19.1‰ to 3.0‰ [44,45,46]. Although as it was shown earlier the bottled mineral waters usually have insignificant differences in deuterium content and even smaller fluctuations in concentration of oxygen-18, modern technologies allow us to obtain deuterium-depleted water (DDW) (with deuterium content till δ 2 H = −968‰) and oxygen-18 depleted water (reduction till 26.1% in comparison with 18 O VSMOW ) [47,48,49,50,51].…”

Section: Introductionmentioning

confidence: 99%

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

et al. 2019

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This review article presents data about the influence of deuterium-depleted water (DDW) on biological systems. It is known that the isotope abundances of natural and bottled waters are variable worldwide. That is why different drinking rations lead to changes of stable isotopes content in body water fluxes in human and animal organisms. Also, intracellular water isotope ratios in living systems depends on metabolic activity and food consumption. We found the 2H/1H gradient in human fluids (δ2H saliva >> δ2H blood plasma > δ2Hbreast milk), which decreases significantly during DDW intake. Moreover, DDW induces several important biological effects in organism (antioxidant, metabolic detoxification, anticancer, rejuvenation, behavior, etc.). Changing the isotope 2H/1H gradient from “2H blood plasma > δ2H visceral organs” to “δ2H blood plasma << δ2H visceral organs” via DDW drinking increases individual adaptation by isotopic shock. The other possible mechanisms of long-term adaptation is DDW influence on the growth rate of cells, enzyme activity and cellular energetics (e.g., stimulation of the mitochondrion activity). In addition, DDW reduces the number of single-stranded DNA breaks and modifies the miRNA profile.

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“…The concentration of deuterium in the produced water was determined by means of a JEOL JNM-ECA 400MHz pulsed nuclear magnetic resonance spectrometer [23] . To determine the isotope D/H composition, liver samples (3.0 ± 0.1 mg each) were preliminarily freeze-dried using a lyophilizer ("LS-1000"; Prointech, Russian Federation) [24] .…”

Section: Ddw Was Produced On a Plant Designed In Kubanmentioning

confidence: 99%

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

Басов

Anatolyevna

Alexandrovich

et al. 2019

ibj

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Background: This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. Methods: This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. Results: Modification of isotope (deuterium-protium [D/H]) composition of rats' blood and organ tissues with DDW (-705‰), introduced into rats' diet within four weeks, led to the formation of isotope D/H gradient between blood plasma and organ tissues and affected isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H 2 O 2 production during the incubation in DDW medium. This increased level of H 2 O 2 production was higher in the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰). Conclusion: The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.

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Application of NMR Spectroscopy to the Determination of Low Concentrations of Nonradioactive Isotopes in Liquid Media

Cited by 16 publications

References 10 publications

Effects of Heavy Isotopes (2H1 and 18O16) Depleted Water Con-Sumption on Physical Recovery and Metabolic and Immunological Parameters of Healthy Volunteers under Regular Fitness Load

Effects of Heavy Isotopes (2H1 and 18O16) Depleted Water Con-Sumption on Physical Recovery and Metabolic and Immunological Parameters of Healthy Volunteers under Regular Fitness Load

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

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