Application of NMR Spectroscopy for Structural Characterization of Bioactive Peptides Derived from Food Protein

Kamran, Fozia; Salampessy, Junus; Reddy, N. Venkata

doi:10.2174/9781681082875116050003

Cited by 5 publications

(6 citation statements)

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“…The presence of numerous peptides with different sequences may also cumulatively affect the ACE inhibitory activity values of various MWCO fractions. These results are in agreement with the results reported in the literature on different protein sources (Kamran, Salampessy, & Reddy, 2016).…”

Section: Resultssupporting

confidence: 93%

“…Consumption of food protein results in beneficial effects on human health. These proteins are derived from animal and plant sources, for example, milk, eggs, cheese, meat, fish, soy, rice, wheat and legumes (Kamran & Reddy, 2018; Kamran, Salampessy, & Reddy, 2016; Korhonen & Pihlanto, 2006; Pritchard, Phillips, & Kailasapathy, 2010). The beneficial effects of these food proteins are enhanced either by gastrointestinal enzymatic hydrolysis or by microbial fermentation to generate peptides with improved biological activities.…”

Section: Introductionmentioning

confidence: 99%

“…The angiotensin converting enzyme (ACE) inhibitors reduce the activity of ACE and produce a vasorelaxing effect on blood vessels (Barbana & Boye, 2011; Kamran, Salampessy, & Reddy, 2016; Kuba, Tana, Tawata, & Yasuda, 2005; Nasri et al, 2013; Paiva, Lima, Neto, & Baptista, 2016; Rho, Lee, Chung, Kim, & Lee, 2009; Shahidi & Zhong, 2008). Therefore, inhibition of ACE is very important in regulating blood pressure in hypertensive subjects (Boschin, Scigliuolo, Resta, & Arnoldi, 2014a; Chin et al, 2019; Hong et al, 2008; Lavoie & Sigmund, 2003; Luna‐Vital, Mojica, González de Mejía, Mendoza, & Loarca‐Piña, 2015; Mäkinen, Streng, Larsen, Laine, & Pihlanto, 2016; Shahidi & Zhong, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Functional properties of Australian blue lupin (Lupinus angustifolius) protein and biological activities of protein hydrolysates

2020

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Lupin is an undervalued legume despite its high protein content with known health benefits. In this research, Australian blue lupin protein was isolated and hydrolysed enzymatically to produce bioactive peptides with a view to assess their potential for nutraceutical and therapeutic applications. Pepsin, pancreatin and flavourzyme were used to enzymatically hydrolyse blue lupin protein, and the hydrolysates were subjected to molecular weight cut‐off (MWCO) fractionation. Measurement of biological activities led to the identification of angiotensin converting enzyme (ACE) inhibitory fractions in the molecular weight range of 2–3 and 3–5 kDa. For the most active fractions in this range, the ACE inhibitory activities were very significant with IC50 values from 450 to 600 μg/ml. Blue lupin protein‐derived MWCO fractions were significantly active against Gram‐positive bacteria and only a little inhibition was observed against Gram‐negative bacteria. Pancreatin hydrolysed fractions showed the best antimicrobial activities with several fractions exhibiting ≥85% inhibition against Bacillus cereus and Staphylococcus aureus. These properties reveal the potential of lupin protein hydrolysates for developing antihypertensive and host defence agents. In order to demonstrate the potential of isolated blue lupin protein in food industry, functional properties including water and oil absorption capacity, gelling properties, solubility and emulsifying properties were evaluated and found to be extremely suitable for developing functional foods with enhanced health benefits.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional properties of Australian blue lupin (Lupinus angustifolius) protein and biological activities of protein hydrolysates

2020

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“…It is important to determine the primary sequence and the secondary structure of peptides as these structural elements are related to their biological activities. Structural characterization of identified bioactive peptides is usually carried out using the mass spectrometry (MS) technique for amino acid sequencing followed by molecular spectroscopy for secondary structure determination [173][174][175][176]. This review specifically focuses on the structure-activity relationship (SAR) aspects of antimicrobial peptides (AMPs), antihypertensive peptides, and antioxidative peptides (AOPs), given the abundance of literature supporting these prediction models.…”

Section: Structure-activity Relationship (Sar)mentioning

confidence: 99%

“…The secondary structures of natural AMPs are predominantly β-sheets and α-helices [183]. However, the literature on the secondary structures of AMPs derived from food proteins is limited [176]. Therefore, limiting the discussion on the SAR of AMPs to natural peptides.…”

Section: Antimicrobial Peptides (Amps)mentioning

confidence: 99%

Exploring the Potential of Bioactive Peptides: From Natural Sources to Therapeutics

Purohit,

Reddy,

Sunna

2024

IJMS

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Bioactive peptides, specific protein fragments with positive health effects, are gaining traction in drug development for advantages like enhanced penetration, low toxicity, and rapid clearance. This comprehensive review navigates the intricate landscape of peptide science, covering discovery to functional characterization. Beginning with a peptidomic exploration of natural sources, the review emphasizes the search for novel peptides. Extraction approaches, including enzymatic hydrolysis, microbial fermentation, and specialized methods for disulfide-linked peptides, are extensively covered. Mass spectrometric analysis techniques for data acquisition and identification, such as liquid chromatography, capillary electrophoresis, untargeted peptide analysis, and bioinformatics, are thoroughly outlined. The exploration of peptide bioactivity incorporates various methodologies, from in vitro assays to in silico techniques, including advanced approaches like phage display and cell-based assays. The review also discusses the structure–activity relationship in the context of antimicrobial peptides (AMPs), ACE-inhibitory peptides (ACEs), and antioxidative peptides (AOPs). Concluding with key findings and future research directions, this interdisciplinary review serves as a comprehensive reference, offering a holistic understanding of peptides and their potential therapeutic applications.

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Soy Proteins as Potential Source of Active Peptides of Nutraceutical Significance

Salampessy

Reddy

2017

Soy Protein‐Based Blends, Composites and Nanocomposites

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Application of NMR Spectroscopy for Structural Characterization of Bioactive Peptides Derived from Food Protein

Cited by 5 publications

References 0 publications

Functional properties of Australian blue lupin (Lupinus angustifolius) protein and biological activities of protein hydrolysates

Functional properties of Australian blue lupin (Lupinus angustifolius) protein and biological activities of protein hydrolysates

Exploring the Potential of Bioactive Peptides: From Natural Sources to Therapeutics

Soy Proteins as Potential Source of Active Peptides of Nutraceutical Significance

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