Application of multifold characteristic ion filtering combined with statistical analysis for comprehensive profiling of chemical constituents in anti-renal interstitial fibrosis I decoction by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap high resolution mass spectrometry

Rapid characterization of chemical constituents of Shaoyao Gancao decoction using UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

“…1 However, it is difficult for researchers to explain the component that plays a major role in the efficacy of the materials because of their massive chemical composition, which is an obstacle for TCM in the international market. 2 In reaction to this phenomenon, multiple studies have focused on examining the chemical components of TCM. However, TCM is numerous in quantity and complicated in composition, so the condition of current research is far from enough.…”

A Metabolomics Coupled With Chemometrics Strategy to Filter Combinatorial Discriminatory Quality Markers of Crude and Salt-Fired Eucommiae Cortex

“…Lignans and their derivatives were a main class of secondary metabolites in Eucommiae Cortex, and display various bioactivities in vivo or in vitro (Deyama, 1983;Shi et al, 2013). In this work, 31 lignans have been tentatively characterized, including compounds 16, 24-25, 28-30, 34-37, 42-47, 49-52, 54, 55, 57-65 (Brenes et al, 2000;Guo et al, 2007;Feng et al, 2007;Chai et al, 2012;Pi et al, 2016;He et al, 2018;Jia et al, 2019;Jiang et al, 2019;Qi et al, 2019). The most lignans in Eucommiae Cortex are phenylpropanoid dimers with one or two glucose units, which means a few of the MS 2 fragments followed by the loss of glucose neutral moiety.…”

“…Compared the real retention behavior with that in the reported literatures (He et al, 2018), compounds 42 and 52 were tentatively identified as erythroguaiacylglycerol-b-conifery aldehyde ether and threoguaiacylglycerol-b-conifery aldehyde ether, respectively. Although several lignans such as compounds 25, 30, 34, 43, 44, and 49 could not be found based on the characteristic ions, they were also tentatively identified by comparing with the precise parent ions (error below 5 ppm), MS 2 fragment ions and the retention behavior with the data obtained in literatures (He et al, 2018;Jiang et al, 2019).…”

“…The most lignans in Eucommiae Cortex are phenylpropanoid dimers with one or two glucose units, which means a few of the MS 2 fragments followed by the loss of glucose neutral moiety. Moreover, the MS 2 spectrum of lignans showed several key characteristic ions at m/z 327, 311, 181, and 150, which were mainly attributed to cleavage of the tetrahydrofuran ring and losses of CH 3 , CH 2 O, CO, CH 3 O, and CH 3 OH (Guo et al, 2007;Jiang et al, 2019). Take several compounds for examples to illustrate the qualitative process.…”

<p>Emodin Retarded Renal Fibrosis Through Regulating HGF and TGFβ–Smad Signaling Pathway</p>

“…9,10 In addition, Rheum officinale has been confirmed to have some bioactivity, including antifibrotic activity. 11,12 Emodin is the major bioactive compound of R. officinale and has strong anti-inflammatory and antioxidant properties. 13 It has been reported that emodin notably suppresses the development of multiple diseases.…”