Application of molecularly imprinted polymers in the anti-doping field: sample purification and compound analysis

Hand, Rachel A.; Piletska, Elena; Bassindale, Thomas; Morgan, G. H.; Turner, Nicholas W.

doi:10.1039/d0an00682c

Cited by 10 publications

(4 citation statements)

References 165 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A MIP is typically created when a polymer is formed from monomer constituents about an analyte (or analyte surrogate), thus creating an analyte-shaped cavity. While this methodology generally requires stoichiometric access to analyte, the use of boronic acid (and related) monomers , in this process can deliver materials with incredibly high spatial and chemical specificity for a chosen saccharide-containing analyte, with features akin to antigen–antibody relationships. , Good progress has been made in the detection of glycoprotein biomarkers − as well as the detection of smaller saccharides. − Combining the benefits of MIPs with molecular chemosensors may offer even more promise for wider ranging applications in the future.…”

Section: Boronic Acid-based Saccharide Sensor Designmentioning

confidence: 99%

Molecular Boronic Acid-Based Saccharide Sensors

2021

View full text Add to dashboard Cite

Boronic acids can reversibly bind diols, a molecular feature that is ubiquitous within saccharides, leading to their use in the design and implementation of sensors for numerous saccharide species. There is a growing understanding of the importance of saccharides in many biological processes and systems; while saccharide or carbohydrate sensing in medicine is most often associated with detection of glucose in diabetes patients, saccharides have proven to be relevant in a range of disease states. Herein the relevance of carbohydrate sensing for biomedical applications is explored, and this review seeks to outline how the complexity of saccharides presents a challenge for the development of selective sensors and describes efforts that have been made to understand the underpinning fluorescence and binding mechanisms of these systems, before outlining examples of how researchers have used this knowledge to develop ever more selective receptors.

show abstract

Section: Boronic Acid-based Saccharide Sensor Designmentioning

confidence: 99%

Molecular Boronic Acid-Based Saccharide Sensors

2021

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the synthesis of molecularly imprinted polymers (MIPs) was boosted by Wulff and Mosbach later in the 1970s [ 22 , 23 ]. Since then, a broad spectrum of analytes including low-molecular-weight molecules, such as pharmaceuticals, sugars, toxins, narcotic drugs, pesticides, and biomacromolecules such as proteins, nucleic acids, bacteria, and viruses have been described [ 20 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ].…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?

Yarman

Kurbanoğlu

2022

Biomimetics

View full text Add to dashboard Cite

Since the first reported case of COVID-19 in 2019 in China and the official declaration from the World Health Organization in March 2021 as a pandemic, fast and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has played a major role worldwide. For this reason, various methods have been developed, comprising reverse transcriptase-polymerase chain reaction (RT-PCR), immunoassays, clustered regularly interspaced short palindromic repeats (CRISPR), reverse transcription loop-mediated isothermal amplification (RT-LAMP), and bio(mimetic)sensors. Among the developed methods, RT-PCR is so far the gold standard. Herein, we give an overview of the MIP-based sensors utilized since the beginning of the pandemic.

show abstract

“…The use of dummy MIPs (DMIPs) has become a choice of interest to avoid inaccurate quantitation of the analyte in real samples due to bleeding or leaching of the unwashed template remnants into the sample matrix. DMIPs are prepared using an analog molecule of the target analyte as a dummy template (DT) [ 11 ]. To the best of our knowledge, no MISPE method has been reported for the isolation of STZ metabolites from human urine using STZ as a DT.…”

Section: Introductionmentioning

confidence: 99%

Novel dummy molecularly imprinted polymer for simultaneous solid-phase extraction of stanozolol metabolites from urine

Farag,

Hanafi,

Hammam

2024

Anal Bioanal Chem

View full text Add to dashboard Cite

Stanozolol, a synthetic derivative of testosterone, is one of the common doping drugs among athletes and bodybuilders. It is metabolized to a large extent and metabolites are detected in urine for a longer duration than the parent compound. In this study, a novel dummy molecularly imprinted polymer (DMIP) is developed as a sorbent for solid-phase extraction of stanozolol metabolites from spiked human urine samples. The optimized DMIP is composed of stanozolol as the dummy template, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker in a ratio of 1:10:80. The extracted analytes were quantitively determined using a newly developed and validated ultrahigh-performance liquid chromatography tandem mass spectrometry method, where the limits of detection and quantitation were 0.91 and 1.81 ng mL−1, respectively, fulfilling the minimum required performance limit decided on by the World Anti-Doping Agency. The mean percentage extraction recoveries for 3’-hydroxystanozolol, 4β-hydroxystanozolol, and 16β-hydroxystanozolol are 97.80% ± 13.80, 83.16% ± 7.50, and 69.98% ± 2.02, respectively. As such, the developed DMISPE can serve as an efficient cost-effective tool for doping and regulatory agencies for simultaneous clean-up of the stanozolol metabolites prior to their quantification. Graphical abstract

show abstract

Application of molecularly imprinted polymers in the anti-doping field: sample purification and compound analysis

Abstract: A detailed review focussing on the WADA prohibited list, exploring the potential application of molecularly imprinted polymers in anti-doping.

Cited by 10 publications

References 165 publications

Molecular Boronic Acid-Based Saccharide Sensors

Molecular Boronic Acid-Based Saccharide Sensors

Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?

Novel dummy molecularly imprinted polymer for simultaneous solid-phase extraction of stanozolol metabolites from urine

Contact Info

Product

Resources

About