Application of microwave assisted three phase partitioning method for purification of laccase from Trametes hirsuta

Patil, Pravin D.; Yadav, Ganapati D.

doi:10.1016/j.procbio.2017.10.006

Cited by 40 publications

(8 citation statements)

References 52 publications

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“…The alcohol used for UATPP was set as t-butanol as it is commonly used in past studies (Yadav et al, 2017; Patil and Yadav, 2018). Dhananjay and Mulimani (2009) have stated that at room temperature, t-butanol tends to be kosmotropic, contributing to enzyme-t-butanol co-precipitates that floats above the bottom phase (Dhananjay and Mulimani, 2009).…”

Section: Resultsmentioning

confidence: 99%

Microalgal Protein Extraction From Chlorella vulgaris FSP-E Using Triphasic Partitioning Technique With Sonication

Chia

Chew

Zaid

et al. 2019

Front. Bioeng. Biotechnol.

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Green microalgae containing various bioactive compounds and macronutrients such as lipids, carbohydrates, and proteins, have attracted much attention from the global community. Microalgae has the potential to be applied in food industries due to its high protein content, rapid growth rate, and ability to survive in harsh conditions. This study presents a simple yet efficient technique of sonication-assisted triphasic partitioning process, also known as ultrasonic-assisted three phase partitioning (UATPP), for the extraction of proteins from Chlorella vulgaris FSP-E. Comparison studies between three phase partitioning (TPP) and UATPP was conducted to investigate the feasibility of the enhanced technique on proteins extraction. Types of salt, ratio of slurry to t-butanol, salt saturation, sonication frequency, power, irradiation time, and duty cycle as well as biomass loading were studied. UATPP was found to be an improved technique compared to TPP. An optimum separation efficiency and yield of 74.59 ± 0.45 and 56.57 ± 3.70% was obtained, respectively, with the optimized conditions: salt saturation (50%), slurry to t-butanol ratio (1:2), sonication power (100%), irradiation time (10 min), frequency (35 kHz), duty cycle (80%) and biomass loading (0.75 wt%). A scaled-up study was performed to validate the reliability of UATPP for protein extraction. The outcome of the study revealed that UATPP is an attractive approach for downstream processing of microalgae.

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Section: Resultsmentioning

confidence: 99%

Microalgal Protein Extraction From Chlorella vulgaris FSP-E Using Triphasic Partitioning Technique With Sonication

Chia

Chew

Zaid

et al. 2019

Front. Bioeng. Biotechnol.

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“…Biochar production through microwave heating is a prominent advanced technique. Microwave heating is advantageous over conventional heating as microwave generates thermal energy through dielectric heating, and the energy is introduced into the reactor remotely without making any contact between the energy source and the reaction mixture [122][123][124]. It is a more rapid and material-selective heating technique than the conventional one [125,126].…”

Section: Microwave Pyrolysismentioning

confidence: 99%

Recent trends in biochar production methods and its application as a soil health conditioner: a review

et al. 2020

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Interest in biochar production from organic waste has been growing in recent years due to its broad applicability, availability, and smoother production. Biochar production techniques are being continuously modernized to improve the production rate and quality. Though numerous methods have been reported in the recent past, a systematic classification of the same is yet to be explored. Based on the advancement of the techniques being employed for biochar production and modification of conventional methods, we have categorized all major techniques of biochar production into two primary classes. In the traditional approach, ancient methods and conventional pyrolysis techniques (Slow and Fast pyrolysis) are included, whereas, in modern approaches, several advanced technologies such as Gasification, Torrefaction, Hydrothermal carbonization, Electro-modification, along with modified traditional methods (Flash pyrolysis, Vacuum pyrolysis, and Microwave pyrolysis) are comprised. Further, the systematic review was intended to evaluate various types of feedstocks (agricultural biomass, forest/woody biomass, aquatic biomass, urban waste, and paper waste) with their potential to produce biochar. It was observed that the feedstock containing high cellulose was found to be helpful in improving the overall properties of biochar, including enhanced adsorptive action and retention of nutrients.

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“…Laccases are widely distributed in fungi, insects, plants, and bacteria [14][15][16][17][18]. Many laccases have been reported from genus Trametes including T. versicolor [9], T. trogii [19], T. hirsuta [20], T. polyzona [21], T. maxima [22] and Trametes sp. [23].…”

Section: It Catalyzes H 2 O 2 -Dependent Oxidation Of Mn 2þmentioning

confidence: 99%

Two Manganese Peroxidases and a Laccase ofTrametes polyzonaKU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

et al. 2019

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Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from Trametes polyzona KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25 mg/L) in 10-30 min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were 50 C and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.

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Application of microwave assisted three phase partitioning method for purification of laccase from Trametes hirsuta

Cited by 40 publications

References 52 publications

Microalgal Protein Extraction From Chlorella vulgaris FSP-E Using Triphasic Partitioning Technique With Sonication

Microalgal Protein Extraction From Chlorella vulgaris FSP-E Using Triphasic Partitioning Technique With Sonication

Recent trends in biochar production methods and its application as a soil health conditioner: a review

Two Manganese Peroxidases and a Laccase ofTrametes polyzonaKU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

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