Application of Microfluidics in Biosensors

Wang, Jing; Ren, Yong; Zhang, Bei

doi:10.5772/intechopen.91929

Cited by 17 publications

(12 citation statements)

References 100 publications

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“…Diverse inorganic (e.g., glass, silicon, and ceramics), polymeric (e.g., elastomers and thermoplastics), and emerging paper (e.g., cellulose) based materials can be used to fabricate microfluidic devices, depending on the required function, degree of the integration and applications ( Nge et al, 2013 ). Previously studied microfluidic based biosensors majorly used the elastomer polydimethylsiloxane (PDMS) as the substrate ( Volpetti et al, 2017 ; Wan et al, 2019b ; Wang J. et al, 2020 ). PDMS has many superiorities for the fabrication of microfluidic biosensors including its reasonable cost, the ability for achieving rapid and easy prototyping, the capacity of enabling multiple layers design to create complex fluidics, and its function for supporting important microfluidic components (e.g., pneumatic valves and pumps).…”

Section: Fabricating Mwcb To Display Multiple Contaminants Levelsmentioning

confidence: 99%

Microfluidic Based Whole-Cell Biosensors for Simultaneously On-Site Monitoring of Multiple Environmental Contaminants

Cao

Zhang

Zhu

et al. 2021

Front. Bioeng. Biotechnol.

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Monitoring of environmental contaminants serves a vital role in proactive environmental management and pollution control. Research efforts have been centered on the development of robust whole-cell biosensors in recent years. However, data acquisition, multiple contaminants detection and biosafety issues limit the on-site application of such biosensors. Microfluidic system exhibits great potential to face these challenges via coupling biosensors. Here, we prospect a novel microfluidic based whole-cell biosensor (MWCB) for multiplexing monitoring of diverse contaminants, and design strategies to further increase the specificity, sensitivity and accuracy, reduce signal delay and expand shelf life of the proposed MWCB for on-site environmental applications. The development of MWCB demands multidisciplinary cooperation, and the sensing platforms are highly promising for real-world contaminants monitoring.

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Section: Fabricating Mwcb To Display Multiple Contaminants Levelsmentioning

confidence: 99%

Microfluidic Based Whole-Cell Biosensors for Simultaneously On-Site Monitoring of Multiple Environmental Contaminants

Cao

Zhang

Zhu

et al. 2021

Front. Bioeng. Biotechnol.

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“…According to the definition provided by Whitesides from Harvard University, “microfluidic is the science and technology of systems that process or manipulate small amounts (10 −9 to 10 −18 L) of fluids, using channels with dimensions of tens to hundreds of micrometers”. This technique shows a great potential to control the concentrations of molecules in space and time [ 82 ]. High surface-to-volume ratios, small consumption of reagents, prevalence of viscous and capillary forces and laminar flows are the major features of microfluidic-based systems [ 83 ].…”

Section: Colorimetric Strategies For Mycotoxins Detectionmentioning

confidence: 99%

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Majdinasab

Aissa

Marty

2020

Toxins

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Mycotoxins contamination is a global public health concern. Therefore, highly sensitive and selective techniques are needed for their on-site monitoring. Several approaches are conceivable for mycotoxins analysis, among which colorimetric methods are the most attractive for commercialization purposes thanks to their visual read-out, easy operation, cost-effectiveness, and rapid response. This review covers the latest achievements in the last five years for the development of colorimetric methods specific to mycotoxins analysis, with a particular emphasis on their potential for large-scale applications in food industries. Gathering all types of (bio)receptors, main colorimetric methods are critically discussed, including enzyme-linked assays, lateral flow-assays, microfluidic devices, and homogenous in-solution strategies. This special focus on colorimetry as a versatile transduction method for mycotoxins analysis is comprehensively reviewed for the first time.

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“…Since 1990, with the advent of microfluidics, the development of microfluidicintegrated biosensors has been intensive due to their potential in early diagnosis, on-site and routine analysis, and high selectivity [3]. Microfluidics also improve sensitivity by providing a more stable sensing environment, integrating multiple functions, and enhancing efficiency, accuracy, and controllability while reducing the sensing region [4][5][6]. The by providing a more stable sensing environment, integrating multiple functions, and enhancing efficiency, accuracy, and controllability while reducing the sensing region [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidics also improve sensitivity by providing a more stable sensing environment, integrating multiple functions, and enhancing efficiency, accuracy, and controllability while reducing the sensing region [4][5][6]. The by providing a more stable sensing environment, integrating multiple functions, and enhancing efficiency, accuracy, and controllability while reducing the sensing region [4][5][6]. The attention towards microfluidic-integrated biosensors is enhanced extra by its capability of detecting a low concentration of biomarkers [7,8].…”

Section: Introductionmentioning

confidence: 99%

Flow Control Techniques for Enhancing the Bio-Recognition Performance of Microfluidic-Integrated Biosensors

et al. 2021

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Biosensors are favored devices for the fast and cost-effective detection of biological species without the need for laboratories. Microfluidic integration with biosensors has advanced their capabilities in selectivity, sensitivity, controllability, and conducting multiple binding assays simultaneously. Despite all the improvements, their design and fabrication are still challenging and time-consuming. The current study aims to enhance microfluidic-integrated biosensors’ performance. Three different functional designs are presented with both active (with the help of electroosmotic flow) and passive (geometry optimization) methods. For validation and further studies, these solutions are applied to an experimental setup for DNA hybridization. The numerical results for the original case have been validated with the experimental data from previous literature. Convection, diffusion, migration, and hybridization of DNA strands during the hybridization process have been simulated with finite element method (FEM) in 3D. Based on the results, increasing the velocity on top of the functionalized surface, by reducing the thickness of the microchamber in that area, would increase the speed of surface coverage by up to 62%. An active flow control with the help of electric field would increase this speed by 32%. In addition, other essential parameters in the fabrication of the microchamber, such as changes in pressure and bulk concentration, have been studied. The suggested designs are simple, applicable and cost-effective, and would not add extra challenges to the fabrication process. Overall, the effect of the geometry of the microchamber on the time and effectiveness of biosensors is inevitable. More studies on the geometry optimization of the microchamber and position of the electrodes using machine learning methods would be beneficial in future works.

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Application of Microfluidics in Biosensors

Cited by 17 publications

References 100 publications

Microfluidic Based Whole-Cell Biosensors for Simultaneously On-Site Monitoring of Multiple Environmental Contaminants

Microfluidic Based Whole-Cell Biosensors for Simultaneously On-Site Monitoring of Multiple Environmental Contaminants

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

Flow Control Techniques for Enhancing the Bio-Recognition Performance of Microfluidic-Integrated Biosensors

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