Microfluidic Based Whole-Cell Biosensors for Simultaneously On-Site Monitoring of Multiple Environmental Contaminants

Cao, Yiqi; Zhang, Baiyu; Zhu, Zhiwen; Xin, Xiaying; Wu, Hongjing; Chen, Bing

doi:10.3389/fbioe.2021.622108

Cited by 24 publications

(8 citation statements)

References 36 publications

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“…Thus, WCBs discussed here have great potential for environmental pollution monitoring. The next level in the field of WCBs has now been achieved, where researchers have now combined WCBs with multiplexed microfluidics-based devices, 67 which can effectively reduce the delay in signal processing and help in simultaneous near real-time pollutant monitoring. Since these devices are easy to operate, highly sensitive, and selective, and can be made into cheap portable devices, the technology is gaining popularity.…”

Section: Cell-based Biosensors: a Sensitive And Robust Approachmentioning

confidence: 99%

Harnessing the Potential of Biological Recognition Elements for Water Pollution Monitoring

2022

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Environmental monitoring of pollutants is an imperative first step to remove the genotoxic, embryotoxic, and carcinogenic toxins. Various biological sensing elements such as proteins, aptamers, whole cells, etc., have been used to track down major pollutants, including heavy metals, aromatic pollutants, pathogenic microorganisms, and pesticides in both environmental samples and drinking water, demonstrating their potential in a true sense. The intermixed use of nanomaterials, electronics, and microfluidic systems has further improved the design and enabled robust on-site detection with enhanced sensitivity. Through this perspective, we shed light on the advances in the field and entail recent efforts to optimize these systems for real-time, online sensing and on-site field monitoring.

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Section: Cell-based Biosensors: a Sensitive And Robust Approachmentioning

confidence: 99%

Harnessing the Potential of Biological Recognition Elements for Water Pollution Monitoring

2022

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“…For example, the enzymes such as AChE have a similar response to different AChE-inhibiting compounds, which led to a low selectivity among the same types of contaminants. To enhance the selectivity of enzyme-based biosensors, microfluidic devices and permseletive memberanes can be used as the pretreatment step to isolate contaminants (Cao et al, 2021). Aptamers and antibodies can be tailored by the specific analyte to increase the selectivity of biosensors.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

et al. 2022

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Water contamination by pathogens and organic pollutants is one of the major environmental problems that risk human health. Climate change with extreme weather can promote their prevalence in waters. Environmental monitoring of these pollutants in a fast, continuous, and accurate manner is of increasing demand, especially under the climate change context, but is challenged by their ubiquity and trace concentrations. Optical biosensing is one of the desired solutions owing to its rapid and accurate detection with high sensitivity. Principally, an optical biosensor recognizes these bioactive toxins and contaminants by tailored bioreceptors (e.g., aptamer, enzyme, and cells) and transduces the biological response to optical signals. Research efforts have been made on tailoring bioreceptors and enhancing signal transducing by nanoparticles. This study comprehensively reviewed the mechanisms of optical biosensing and the recent development of bioreceptors and nanomaterials on the enhancement for the rapid, easy, and accurate analysis of emerging contaminants in water. The advantages and challenges on sensitivity, selectivity, and durability of biosensors were discussed along with the opportunities and development strategies.

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“…In recent years, microfluidic technology has attracted considerable interest in CTCs detection. Microfluidic technology is characterized by a micro-manufacturing structure, which usually manipulates the fluid with high flux and sensitivity on the micron scale ( Cao et al, 2021 ). With the remarkable progress of micro-machining methods, the microfluid platform has significant advantages such as low cost, good micro-structure, reduced sample consumption, rapid fluid processing, good detection sensitivity, and so on, and is applied to the primary and applied research of oncology ( Lin et al, 2020 ; Pei et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%

Application of Microfluidics in Detection of Circulating Tumor Cells

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Tumor metastasis is one of the main causes of cancer incidence and death worldwide. In the process of tumor metastasis, the isolation and analysis of circulating tumor cells (CTCs) plays a crucial role in the early diagnosis and prognosis of cancer patients. Due to the rarity and inherent heterogeneity of CTCs, there is an urgent need for reliable CTCs separation and detection methods in order to obtain valuable information on tumor metastasis and progression from CTCs. Microfluidic technology is increasingly used in various studies of CTCs separation, identification and characterization because of its unique advantages, such as low cost, simple operation, less reagent consumption, miniaturization of the system, rapid detection and accurate control. This paper reviews the research progress of microfluidic technology in CTCs separation and detection in recent years, as well as the potential clinical application of CTCs, looks forward to the application prospect of microfluidic technology in the treatment of tumor metastasis, and briefly discusses the development prospect of microfluidic biosensor.

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Microfluidic Based Whole-Cell Biosensors for Simultaneously On-Site Monitoring of Multiple Environmental Contaminants

Cited by 24 publications

References 36 publications

Harnessing the Potential of Biological Recognition Elements for Water Pollution Monitoring

Harnessing the Potential of Biological Recognition Elements for Water Pollution Monitoring

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

Application of Microfluidics in Detection of Circulating Tumor Cells

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