Application of microbial phytase in fish feed

Cao, Ling; Wang, Weimin; Yang, Chengtai; Yang, Yi; Diana, James S.; Yakupitiyage, Amararatne; Luo, Zhi; Li, Dapeng

doi:10.1016/j.enzmictec.2007.01.007

Cited by 305 publications

(323 citation statements)

References 67 publications

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“…Due to increasing demands with the passage of time, plant by-products are being used as the alternative protein sources in fish feeds but unfortunately they have antinutritional factor (Liu et al, 2013). To overcome this problem supplementation of phytase in fish feeds has been generally resulted to improve the bioavailability and utilization of plant minerals by fish (Cao et al, 2007). In the current study, the maximum values in terms of minerals digestibility for C. mrigala fingerlings fed on corn gluten (30%) meal-based test diets were at 500 FTU kg -1 with 5% CA supplemented diet followed by 1000 FTU kg -1 with 5% CA and they were significantly (P<0.05) from other test diets used.…”

Section: Discussionmentioning

confidence: 99%

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Hussain¹,

Ahmad²,

Javid³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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Phytic acid is a major part of oilseed meals which reduces bioavailability of minerals and protein to fish. The present research project was planned to check the effects of citric acid and phytase supplementation in corn gluten (30%) meal-based diets, on mineral digestibility of Cirrhinus mrigala fingerlings. The diets were formulated to supply adequate levels of all required nutrients for normal fish growth. Feces were collected twice a day from each tank. Effect of each treatment on the minerals digestibility was calculated using standard formulae. Highest apparent digestibility coefficient % of minerals (Calcium 68%, Phosphorus 77%, Sodium 64%, Potassium 62%, Magnesium 53%, Iron 64%, Copper 68%, Manganese 67% and Zinc 74%) was observed in the fish fed at 5% citric acid and 500 FTUkg -1 phytase in corn gluten (30%) meal-based diet. Results of the current study indicated that addition of 5% citric acid and 500 FTUkg -1 phytase to corn gluten (30%) mealbased diet was most effective among the levels to release the chelated minerals to Cirrhinus mrigala from phytate complexes. Hence, the use of citric acid (5%) and phytase (500 FTUkg -1 ) improves the fish performance when both were used in corn gluten (30%) meal-based diets.

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Section: Discussionmentioning

confidence: 99%

“…Mono gastric and a-gastric fishes do not produce this enzyme so they cannot hydrolyze the phytate. Supplementation of phytase in fish feeds has been generally resulted to improve the bioavailability and utilization of plant phosphorus by fish (Cao et al, 2007;Hussain et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Hussain¹,

Ahmad²,

Javid³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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“…Sama halnya dengan B. clausii, B. amyloliquefaciens merupakan jenis bakteri Gram positif berbentuk batang, seringkali membentuk rantai panjang, bersifat motil dan dapat membentuk spora . B. amylolyquefaciens juga dikenal sebagai salah satu jenis bakteri probiotik yang mampu memproduksi beberapa jenis enzim ekstra seluler seperti amilase (Gangadharan et al, 2006;Basma et al, 2015), fitase (Cao et al, 2007) dan selulase (Wizna et al, 2009). B. amylolyquefaciens memiliki aktivitas enzim selulase seperti eksoglukanase sebesar 1,200 U/ml dan endoglukanase sebesar 0,488 U/ml (Wizna et al, 2009) dan antimikroba (Nastro et al, 2013).…”

Section: Figure 4 the Morphology Of Ug3 (Left) Ug7 (Right) And Ug8 unclassified

“…B. subtilis dilaporkan memiliki kemampuan memproduksi selulase, yaitu CMCase dan avicelase saat ditumbuhkan pada media yang mengandung karbohidrat (Chan & Au, 1987), fitase (Cao et al, 2007), hemiselulase (pullulanase, endo--1,4-mannase dan endo--1,4-xilanase) (Govender et al, 2009), -amylase (Oyeleke et al, 2011), kitinase (Narasimhan et al, 2013), serta pectat lyase (Al Balaa et al, 2014) .…”

Section: Figure 4 the Morphology Of Ug3 (Left) Ug7 (Right) And Ug8 unclassified

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

Mulyasari

Widanarni

Suprayudi

et al. 2015

JPBKP

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ABSTRAKPemanfaatan daun singkong sebagai bahan baku pakan ikan belum dilaksanakan secara optimal karena mengandung selulosa yang cukup tinggi. Tuj uan penelitian ini adalah mendapatkan bakteri yang dapat mendegradasi serat daun singkong dan melakukan identifikasi jenis bakteri selulolitik tersebut. Seleksi bakteri selulolitik dilakukan pada 4 jenis isolat bakteri asal saluran pencernaan ikan gurame yaitu UG3, UG6, UG7, dan UG8 dengan mengukur aktivitas enzim selulase secara kuantitatif pada substrat Carboxy Methyl Cellulose (CMC) dan daun singkong menggunakan metode 3,5-dinitrosalicylic acid (DNS). Identifikasi bakteri dilakukan pada 3 isolat yang memiliki aktivitas enzim selulase tertinggi pada substrat daun singkong berdasarkan morfologi (perwarnaan Gram, motilitas dan bentuk bakteri), uji biokimia (oksidase dan katalase) dan analisis gen (16S-rRNA). Hasil penelitian menunjukkan bahwa dari 4 isolat yang diuji cobakan, isolat UG7 memiliki aktivitas enzim selulase (CMCase) tertinggi (0,0043 U/ ml) sedangkan yang terendah adalah UG3 (0,0018 U/ml). Pada uji coba di substrat daun singkong, aktivitas enzim tertinggi terlihat pada isolat UG3 (0,107 U/ml) dan terendah adalah UG6 (0,077 U/ ml). Hasil identifikasi dari 3 jenis isolat bakteri uji yang memiliki kemampuan mendegradasi daun singkong tertinggi (UG3, UG7, dan UG8) menunjukkan bahwa ketiga jenis isolat tersebut merupakan bakteri jenis Gram positif berbentuk batang (basil). Berdasarkan data base dari GenBank, isolat UG3 memiliki kemiripan sebesar 93% dengan Bacillus clausii, UG7 memiliki kemiripan sebesar 96% dengan Bacillus amyloliquefaciens dan UG8 memiliki kemiripan sebesar 88% dengan Bacillus subtilis.

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“…Other method(s) to ensure the complete elimination of phytate is therefore required. A method currently applied to overcome the challenge of phytate, is the use of phytase in breaking down the phytate-nutrient complexes to enhance release for the target organism [8]. Phytase have been applied into diets to liberate phytate phosphorus and make more utilizable phosphorus available for fish growth.…”

Section: Introductionmentioning

confidence: 99%

The Growth and Mineral Utilization of Clarias Gariepinus Fingerlings Fed Phytase-Supplemented Toasted Lima Bean (Phaseolus lunatus) Diets

EK¹

2015

J Aquac Res Development

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Toasting causes slight reduction in the phytate content of Lima bean, thus requiring further treatment for effective utilization when incorporated into fish feed. The growth, mineral utilization and liver response of Clarias gariepinus fingerlings fed Toasted Lima Bean Meal (TLBM) diets supplemented with phytase was investigated. An Isonitrogenous diet (40% crude protein) was formulated with TLBM as a plant protein source. Phytase was added to diets post pelleting at 0 FTU (F1), 2500 FTU (F2), 5000FTU (F3), 7500FTU (F4) and 10,000FTU (F5). Feed were fed to triplicate groups of 15 fish (1.43 g ± 0.0012 g) stocked in 25 liter capacity plastic tanks, cultured at a mean dissolved oxygen, pH and temperature of 5.37mg/l, 7.2 and 25.8°C respectively for 56 days. Mean weight gain and Feed conversion Ratio (FCR) were significantly (P<0.05) higher in fish fed diets F3 and F4. Fish fed diets F4 had the highest Specific Growth Rate (SGR) 3.31, Protein Efficiency Ratio (PER) 1.78 and least FCR 1.41. The control (F1) gave the lowest SGR 2.79, PER 1.57 and highest FCR 1.6. There is increased phosphorus utilization with increased enzyme inclusion. Bone ash and phosphorus of fish showed mark increase with increasing level of phytase. Histopathological examination of fish revealed no negative effect of phytase on the liver of fish.

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Application of microbial phytase in fish feed

Cited by 305 publications

References 67 publications

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Untitled

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

The Growth and Mineral Utilization of Clarias Gariepinus Fingerlings Fed Phytase-Supplemented Toasted Lima Bean (Phaseolus lunatus) Diets

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