2014
DOI: 10.1371/journal.pone.0086428
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Application of Microarray and Functional-Based Screening Methods for the Detection of Antimicrobial Resistance Genes in the Microbiomes of Healthy Humans

Abstract: The aim of this study was to screen for the presence of antimicrobial resistance genes within the saliva and faecal microbiomes of healthy adult human volunteers from five European countries. Two non-culture based approaches were employed to obviate potential bias associated with difficult to culture members of the microbiota. In a gene target-based approach, a microarray was employed to screen for the presence of over 70 clinically important resistance genes in the saliva and faecal microbiomes. A total of 14… Show more

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Cited by 56 publications
(58 citation statements)
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“…This indicates that multidrug resistance is possibly common in the microbiota of the healthy human population from Sweden, a finding which we previously noted from the United Kingdom (6). Commonly detected genes included bla TEM and sul2, both of which usually reside on plasmids or transposons and are widespread in the human microbiota (5,6,8,10,12,25). Many of the other microarray-positive genes have been described previously in the microbiota of healthy humans (5,6,8,10,12,25).…”
Section: Discussionsupporting
confidence: 68%
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“…This indicates that multidrug resistance is possibly common in the microbiota of the healthy human population from Sweden, a finding which we previously noted from the United Kingdom (6). Commonly detected genes included bla TEM and sul2, both of which usually reside on plasmids or transposons and are widespread in the human microbiota (5,6,8,10,12,25). Many of the other microarray-positive genes have been described previously in the microbiota of healthy humans (5,6,8,10,12,25).…”
Section: Discussionsupporting
confidence: 68%
“…The purified isolates represented many of the natural inhabitants of the human oral and fecal microbiota. All of the fecal anaerobes were identified as Bacteroides, one of the most abundant genera in the human gut (12,23). The majority of the isolated fecal aerobes were E. coli strains, with other Gram-negative species recovered in low numbers, reflecting the normal microbial diversity in the human gut (5,23).…”
Section: Discussionmentioning
confidence: 99%
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