Application of meta-Topolin for improving micropropagation of gooseberry (Ribes grossularia)

Kucharska, D.; Orlikowska, T.; Maciorowski, Robert; Kunka, Małgorzata; Wójcik, Danuta; Pluta, S.

doi:10.1016/j.scienta.2020.109529

Cited by 30 publications

(12 citation statements)

References 21 publications

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“…m T (6-(3-hydroxylbenzylamino) purine) is an aromatic natural cytokinin and isolated from poplar leaves (Chauhan and Taylor, 2018 ; Strnad et al, 1997 ). The effective shoot multiplication with the application of m T has been reported in various plant species (Behera et al 2019 , 2022 ; Halder and Ghosh 2021 ; Kucharska et al 2020 ; Naaz et al 2019 ) and it also showed a higher efficacy over BAP for shoot multiplication in W. somnifera (Kaur et al 2021a ). This could be because the aromatic side chain has a hydroxyl group, which facilitates the formation of O -glycoside.…”

Section: Critical Factors Influencing Micropropagationmentioning

confidence: 92%

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Kaur

Dolker

Behera

et al. 2022

Plant Cell Tiss Organ Cult

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Withania somnifera (L.) Dunal is a valuable medicinal plant in the Solanaceae family. It is commonly known as Ashwagandha and is widely distributed around the globe. It has multiple pharmacological properties owing to the existence of diverse secondary metabolites viz., withanolide A, withanolide D, withaferin A, and withanone. It is in great demand in the herbal industry because of its extensive use. In this background, the major challenge lies in the rapid multiplication of elite cultivars of W. somnifera in order to produce genetically and phytoconstituents uniform plant material for pharmaceutical industries. Thus it is necessary to explore various biotechnological approaches for the clonal mass propagation and synthesis of pharmaceutically important constituents in W. somnifera . Though there are several studies on in vitro propagation on W. somnifera , yet many factors that critically influence the in vitro response and withanolides production need to be fine-tuned in the pretext of the existing knowledge. The current review focuses on the advancements and prospects in biotechnological interventions to meet the worldwide demands for W. somnifera and its bioactive compounds. This update on in vitro studies on W. somnifera will be useful to many researchers, entrepreneurs, and herbal industries looking for its in vitro mass multiplication and scientific utilization.

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Section: Critical Factors Influencing Micropropagationmentioning

confidence: 92%

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Kaur

Dolker

Behera

et al. 2022

Plant Cell Tiss Organ Cult

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“…Plants produced in vitro are juvenilized, which results in the ability for rapid growth, more dy-https://doi.org/10.17221/142/2020-HORTSCI namic biomass gain, and higher rooting efficiency than those produced traditionally through cuttings (Dubois et al 1988;Howard et al 1989;Drew, Smith 1990). In our field experiment, gooseberry genotypes produced in vitro had twice as much shoot growth and bushes that were three times wider, as well as half the percentage of fruiting plants in the second year of growth (Wójcik et al 2020). This indicates that in vitro propagated plants of gooseberry are a proper source of cuttings, but in the first year of fruiting, they are less productive.…”

mentioning

confidence: 77%

“…At the Research Institute of Horticulture, a breeding program aiming to develop new cultivars of gooseberry are underway, as well as experiments are performed on propagation and cultivation, and qualitative assessment of fruits (Pluta 2018). For the purposes of rapid multiplication of selected clones, we have developed an effective method of micropropagation using meta-topolin (m-T), instead of benzylaminopurine (BAP) (Kucharska et al 2020). In the current work, we present the results of experiments carried out on the short-term storage of gooseberry culture at the multiplication stage in slow-growth conditions.…”

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confidence: 99%

Storage of proliferating gooseberry cultures under slow growth conditions

Kucharska¹,

Orlikowska²,

Maciorowski³

et al. 2021

Hort. Sci. (Prague)

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Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.

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“…In vitro cultures of gooseberry shoots were initiated and multiplied based on a method developed previously [11,12]. The shoots were multiplied on media containing salts and vitamins set by Murashige and Skoog (MS) [35]: 100 mg L −1 inositol, 85.45 mg L −1 MgSO 4 , 0.1 mg L −1 gibberellic acid (GA 3 ), 0.1 mg L −1 indole 3-acetic acid (IAA), 0.5 mg L −1 metatopolin (mT), sucrose 30 g L −1 , and Bacto agar 7.0 g L −1 with a pH of 5.8.…”

Section: Culture Medium and Growth Conditionsmentioning

confidence: 99%

“…In vitro propagation of this species is difficult due to the appearance of shoot necrosis during initiation and multiplication stages, lack of shoot elongation, leaf yellowing, and hyperhydration. For the rapid multiplication of selected gooseberry clones, an effective method of micropropagation using meta-topolin (m-T) instead of benzylaminopurine (BAP) was developed [12]. This method can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market.…”

Section: Introductionmentioning

confidence: 99%

Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

2020

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Gooseberry (Ribes grossularia L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market. The aim of presented study was to assess field performance and genetic stability of micropropagated plants (MPs) of four gooseberry cultivars, “Captivator”, “Hinnonmaki Rot”, “Invicta”, and “Resika”. The growth vigor and yield of MPs and plants propagated by standard methods from softwood cuttings (ST) were evaluated in a field experiment. Microscopic observations of the number and length of the stomata of MP and ST plants were carried out. Two DNA-based techniques, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), were used to assess genetic stability of MP plants. For analysis of genetic stability of ST plants, the ISSR technique was applied. For three cultivars, Captivator, Hinnonmaki Rot, and Invicta, the plants’ growth vigor and fruit yield were greater in MP plants than in ST plants. In the case of Resika, most of these parameters were higher in ST plants. Microscopic observations of the stomata indicated a lack of differences in the length between MP and ST plants, while the stomata frequency on leaves of MP plants was higher than that of ST plants. The genetic variability of MP plants, assessed by AFLP, ranged from 0.35% for Hinnonmaki Rot to 2.12% for Resika. The results of ISSR analysis of MP plants showed variability from 0% in the case of Hinnonmaki Rot and Resika to 4% and 8.69% for Captivator and Invicta, respectively. No polymorphism was detected among ST plants of all analyzed gooseberry cultivars.

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Application of meta-Topolin for improving micropropagation of gooseberry (Ribes grossularia)

Cited by 30 publications

References 21 publications

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Storage of proliferating gooseberry cultures under slow growth conditions

Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

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