Application of Lipase from Marine BacteriaBacillus sonorensisas an Additive in Detergent Formulation

Abstract

The efficacy of lipase (triacylglycerol acylhydrolases EC 3.1.1.3) as a detergent additive from a newly isolated marine halophilic bacteria Bacillus sonorensis from marine clams Paphia malabarica collected in the Kalbadevi Estuary, Mumbai, has been assessed and reported. In terms of activity and stability, the lipase exhibited maximum activity in alkaline conditions and was observed to be stable over a temperature range from room temperature to 60°C. The activity of the lipase increased in the presence of surfactants and detergents. Due to these properties of the lipase from marine bacteria, it was used as an additive in detergents to study its efficiency at corn oil removal from fabrics. The washing studies indicated that the efficiency of corn oil removal from the cotton fabrics increased by 20 % when lipase was incorporated in the detergent as compared to the treatment with detergent alone. The lipase was also capable of removing corn oil from natural as well as synthetic fabrics dyed with a respective, preferred class of dyes.Ravindra Adivarekar is currently a professor and the head of the Department of Fibres and Textiles Processing Technology at the Institute of Chemical Technology (ICT), Mumbai, India. His research areas of interest are microbial enzymes for textile processing, detergent formulations, natural dyes and mordants, dyeing and printing of textiles, medical textiles, fiber modification, and energy conservation.J Surfact Deterg (2013) 16:435-443 443

Classification of lipolytic enzymes and their biotechnological applications in the pulping industry

“…Microbial lipases specifically have unlimited potential in commercial applications, such as additives in fine chemicals, wastewater treatment, food processing, cosmetics, detergents, pharmaceuticals, degreasing formulations, paper manufacture, and accelerated degradation of fatty wastes and polyurethane (Rodriques et al 2016;Shu et al 2016;Speranza et al 2016;Fulton et al 2015;Adulkar and Rathod 2014;Gerits et al 2014;Li et al 2014;Saranya et al 2014;Brabcova et al 2013;Lailaja and Chandrasekaran 2013;Nerurkar et al 2013;Whangsuk et al 2013;Zhang et al 2013;Liu et al 2012;Kamini et al 2000). Even though a large number of lipases have been described in the literature, only for a limited number of enzymes belonging to a few species has it been proven that their stability and biosynthetic activity is amenable for use in organic solvents, and thus for consideration as industrially applicable enzymes (Cardenas et al 2001;Jaeger et al 1999).…”

Effect of ethyleneoxide groups of anionic surfactants on lipase activity

“…The demand for “green” detergents, environmentally friendly, tends to decrease the amount of undesired chemicals in the formulation, replacing them by chemicals with less‐harmful effects on the ecosystem. Enzyme‐based detergents fit all these requirements . The enzymes are expected to act synergistically with surfactants, boosting the cleaning efficiency, and avoiding the use of high concentrations of caustic compounds.…”

“…An efficient enzyme‐based formulation needs a crucial selection of the catalytic protein based on the substrate specificity and stability within the optimal detergent working conditions (e.g., pH and temperature range). Additionally, the interactions between the lipases and other molecules typically present in formulations should be evaluated to highlight possible synergisms and/or compatibility problems . The association with surfactants is expected to occur via the hydrophobic association between the hydrophobic segments of the protein and the hydrophobic tail of the surfactants.…”

Achromobacter sp. and Virgibacillus pantothenticus as models of thermo-tolerant lipase-producing marine bacteria from North Delta sediments (Egypt)

“…Microbial lipases are currently receiving much attention because of their biotechnological potential applications (Aly et al, 2012;Wang et al, 2014). They are the major industrial enzymes extensively used in pharmaceuticals, textiles, food, medical, detergent manufacturing and other chemical industries (Mohan et al, 2008;Sangeetha et al, 2011;Aly et al, 2012;Nerurkar et al, 2013;Chatterjee et al, 2014).…”