Application of LC–ESI–MS–MS for detection of synthetic adulterants in herbal remedies

Bogusz, M; Hassan, Huda; Al-Enazi, Eid; Ibrahim, Zuhour; Al‐Tufail, Mohammed

doi:10.1016/j.jpba.2005.12.015

Cited by 166 publications

(83 citation statements)

References 22 publications

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“…Extraction recovery was also investigated by analyzing one of the commercial products that had been spiked with the standard analytes. The resultant samples were analyzed using the proposed method (Bogusz et al, 2006). Increased chromatographic areas of the analytes in spiked dietary supplement were compared with the areas of the pure standards in methanol (Table 3).…”

Section: Validationmentioning

confidence: 99%

Simultaneous determination of anti‐diabetes/anti‐obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS

Kim

Lee

Yoon

et al. 2009

Biomedical Chromatography

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The safety of dietary supplements is questionable as there have been occasional reports of products contaminated with illegal adulterants. The present study was carried out to develop trustworthy methodologies to screen for six antidiabetic drugs (phenformin, rosiglitazone, glipizide, glimepiride, glybenclamide and gliclazide) and six anti-obesity drugs (ephedrine, fenfluramine, T3, T4, fluoxetine and sibutramine) in dietary supplements. A simultaneous determination method of the 12 drugs by liquid chromatography coupled with a photodiode array (LC/PDA) was established and was validated for linearity (r 2 > 0.99), precision (RSD < < < <13.3%), recoveries (88.8-115.9%) and reproducibility. Sibutramine and its analogs, N-desmethylsibutramine, were subject to further investigation by LC/MS/MS because they were one of the major illegal adulterants. Our proposed method to monitor illegal drug adulterations in dietary supplements using LC/PDA is a simple and reliable, and therefore applicable to routine drug-adulteration screening.

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Section: Validationmentioning

confidence: 99%

Simultaneous determination of anti‐diabetes/anti‐obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS

Kim

Lee

Yoon

et al. 2009

Biomedical Chromatography

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“…Reported methods for screening illegal adulterations in HM or DS are mainly HPLC-DAD, GC-MS and LC/MS/MS (Liu et al 2001;Lau et al 2003;Bogusz et al 2006; Liang et The analytical methods reported concerning more specifically the adulteration of herbal formulations marketed for weight loss are chromatographic methods coupled with various detectors (Tseng et al 2000;Jung et al 2006;Yuen et al 2007;Zou et al 2007;Date et al 2008;Huang et al 2008;Wang et al 2008), capillary electrophoresis (CE) (Cianchino et al 2008) and IR-LSLS (Lu et al 2007). None of these methods is ideal as HM often contain mixture of herbs and other natural products, and the formulas of those products are very complex.…”

mentioning

confidence: 99%

Analysis of adulterated herbal medicines and dietary supplements marketed for weight loss by DOSY¹H-NMR

Vaysse

Balayssac

Gilard

et al. 2010

Food Additives & Contaminants: Part A

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“…Autosampler and quaternary LC pump; this method that detected 20 weight reducing adulterants in THM's; however, Sibutramine was not detected using this method as well (Bogusz et al, 2006).…”

Section: In 2006 Bogusz and Coworkers Performed An Lc-esi-ms-ms Techmentioning

confidence: 92%

“…Figure 2: Chemical structures of (a) sibutramine, (b) N-desmethyl-sibutramine, and (c) N-bisdesmethylsibutramine (Yuen et al, 2007) Although sibutramine has promising results in weight loss, many side effects of the use of sibutramine have been observed such as headaches, insomnia, anorexia, dry mouth, confusion, dizziness, shivering, panic attacks, mood swings, hypertension, tachycardia and arrhythmias; therefore, in 2010, sibutramine was withdrawn and banned from markets due to its serious cardiovascular risks (Deconinck et al, 2014;Yu et al, 2010;Bogusz et al, 2006). Despite this, sibutramine is still detected as an adulterant in THMs and therefore, it is essential to detect its presence.…”

Section: Introductionmentioning

confidence: 99%

The Use of Analytical Techniques to Detect Toxic Synthetic Drug, Sibutramine, Adulterated in Traditional Herbal Medicines

Gadit¹,

Kandiah²

2017

International Conference on Bioscience and Biotechnology

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Traditional herbal medicines (THM's) are still being used worldwide in at least 80% of the population, mainly in developing countries, due to the assumption that they are safer and cheaper. However, these medicines are now being adulterated with synthetic drugs causing toxic effects. One such drug is Sibutramine, a drug used for weight loss. Although Sibutramine has several promising weight loss effects, it causes several toxic side effects; this resulted in its banning and withdrawal from the market. Despite this, it is still being adulterated in herbal medicines and needs to be detected. It is detected using several analytical techniques such as TLC, GC, LC-MS, LC-MS/MS, QTOF-LC/MS and more recently, spectroscopic techniques. Out of all the techniques, QTOF-LC/MS detected concentrations of Sibutramine adulterated in 9.4 mg (not clear what this means) and had LOD and LOQ values of 0.4-2.0 µg kg-1 and 1.3-6.0 µg kg-1 respectively; therefore, QTOF-LC/MS is the best technique developed up to date when compared to all the other techniques. However, research is currently ongoing to combine spectroscopic techniques other than MS, to overcome disadvantages such as lack of global information, false-positive results, time and labor consumption to detect Sibutramine adulteration in the future.

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Application of LC–ESI–MS–MS for detection of synthetic adulterants in herbal remedies

Cited by 166 publications

References 22 publications

Simultaneous determination of anti‐diabetes/anti‐obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS

Simultaneous determination of anti‐diabetes/anti‐obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS

Analysis of adulterated herbal medicines and dietary supplements marketed for weight loss by DOSY¹H-NMR

The Use of Analytical Techniques to Detect Toxic Synthetic Drug, Sibutramine, Adulterated in Traditional Herbal Medicines

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Application of LC–ESI–MS–MS for detection of synthetic adulterants in herbal remedies

Cited by 166 publications

References 22 publications

Simultaneous determination of anti‐diabetes/anti‐obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS

Simultaneous determination of anti‐diabetes/anti‐obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS

Analysis of adulterated herbal medicines and dietary supplements marketed for weight loss by DOSY1H-NMR

The Use of Analytical Techniques to Detect Toxic Synthetic Drug, Sibutramine, Adulterated in Traditional Herbal Medicines

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Analysis of adulterated herbal medicines and dietary supplements marketed for weight loss by DOSY¹H-NMR