Application of Klebsiella oxytoca Biomass in the Biosorptive Treatment of PAH-Bearing Wastewater: Effect of PAH Hydrophobicity and Implications for Prediction

Abstract

Biosorption has been widely recognized as a promising method to treat wastewater. However, few studies have investigated the impact of pollutants' properties on wastewater treatment, as well as the underlying mechanisms and future predictions. In this study, the effects of pollutants' hydrophobicity on the biosorptive removal of polycyclic aromatic hydrocarbons (PAHs) were evaluated. The results showed that the inactive biomass of Klebsiella oxytoca effectively removes PAHs from aqueous solutions with a high biosorption capacity, high biosorption affinity, and short equilibrium time. The biosorption of seven PAHs achieved equilibrium rapidly (less than 2 h) and fitted well to the pseudo-second-order kinetic model. Sorption occurred with a predominantly linear partition process to the biomaterial with K d values of 363.11, 1719.5, 2515.5, 7343.3, 6353.4, 22,806, and 19,541 L·kg −1 for naphthalene, acenaphthene, fluorene, phenanthrene, anthracene, pyrene, and fluoranthene, respectively. An increase in temperature led to a decrease in the biosorption affinity, and the bacterial biosorption of PAHs was spontaneous and exothermic. Furthermore, a positive correlation was observed between the sorption affinity and the octanol partition coefficient (K ow ) (logK d = 1.011logK ow − 0.7369), indicating that hydrophobicity is the main factor influencing the biosorption efficiency. These results suggest that biosorption is an efficient and predictable treatment for micropollutant-bearing wastewater.

The simultaneous and synergistic sorption of phenanthrene and cadmium using biomass derived from rice roots

“…The excitation and emission wavelengths for PHE were 244 and 360 nm, respectively. The limit of detection (LOT) and relative standard deviation (RSD) for PHE were 3.39 μg/L and 0.23%, respectively, as reported in a previous study by the authors (Zhang et al 2018). The concentration of cadmium in the aqueous phase was determined using inductively coupled plasma-optical emission spectrometry (ICP-OES) (Thermo iCAP6300).…”

Synergistic and simultaneous biosorption of phenanthrene and iodine from aqueous solutions by soil indigenous bacterial biomass as a low-cost biosorbent

“…The surface properties of SIBB material were measured according to our previous work. 34 The specific surface of the biosorbent was determined by a gas sorption analyzer (NOVA-1200, Quantachrome Corp., USA). The biosorbent was further characterized using Fourier transform infrared spectroscopy (FTIR, Shimadzu 8400S, Kyoto, Japan).…”

“…The biosorbent was obtained from physically and thermally inactivated soil indigenous bacterial biomass as described in previous works. 31,34 The indigenous bacteria were isolated from soil in an agricultural eld of Zhejiang, China, near the Qinshan Nuclear Power Plant and then cultured in Lysogeny Broth. Aer harvest, bacteria were washed three times with deionized water to remove any remaining culture media.…”

“…The biosorption of HOCs such as phenanthrene onto biomass mainly via hydrophobic interaction due to the presence of aliphatic carbons and aromatic structures, which have been extensively studied. 34,41,42 Choung et al proposed a possible mechanism for the sorption iodine species onto natural organic matters based on XANES spectra. 8 The uptake of iodine associated with the formation of intermediate iodine species through oxidation by quinones and ketones of the black carbon.…”

Optimización de las condiciones de crecimiento de la bacteria endófita Klebsiella oxytoca en matraz y en biorreactor

“…Klebsiella oxytoca ha sido utilizada con fines biotecnológicos en diversas áreas, presenta potencial biorremediador, pues degrada hidrocarburos [11], compuestos orgánicos hidrofóbicos [12], sustancias tóxicas como el cianuro [13], así como insecticidas organoclorados [14], nitrilos [15], ácidos benzoicos [16], colorantes azoicos [17] y el fármaco diclofenaco [18]. Además, se ha reportado la producción de bioelectricidad en conjunto con la degradación de ácidos grasos [19], ha sido capaz de llevar a cabo la desulfuración termofílica de dibenzotiofenos [20], entre otras muchas aplicaciones [21] [22] [23].…”