No abstract

The influence of biochar amendments on the physical quality of a clayey soil (Vertisol) was evaluated by aggregate‐size distribution and stability, water retention, and pore‐space structure of biochar‐amended soils. Clayey soil was treated with three kinds of biochars (straw biochar, woodchips biochar, and wastewater‐sludge biochar) at the rate of 0, 20, 40, and 60 g biochar (kg soil)–1 and incubated for 180 d in glasshouse. The application of straw biochar (SB) and wastewater‐sludge biochar (WSB) significantly enhanced the formation of 5–2 and 0.25–0.5 mm macroaggregates in the clayey soil relative to the control treatment, while the < 0.25‐cm microaggregate decreased with biochar additions. However, woodchips biochar (WCB) had no obvious effect on the formation of macroaggregate. The application of SB and WSB increased the mean weight diameter (MWD) and geometric mean diameter (GMD) of clayey soil, implying that biochar increased the aggregate stability. They improved the aggregate stability through an enhanced resistance to slaking and increased interparticular cohesion. The SB‐amended soils exhibited significant increases in the available water contents of soils. The application of SB significantly increased pore volume in the macropore (> 75 μm) and mesopore (30–75 μm) ranges, which may be the result of the reorganization of pore‐size distribution and aggregation processes induced by the addition of biochar. Results indicated that biochar had the potential to improve the physical quality and pore‐space status of clayey soil. It is suggested that biochar may be considered as a soil amendment for improving poor physical characteristics of clayey soil.

No abstract

Prompt reperfusion after cerebral ischemia is critical for neuronal survival. Any strategies that extend the limited reperfusion window will be of great importance. Acidic postconditioning (APC) is a mild acidosis treatment that involves inhaling CO2 during reperfusion following ischemia. APC attenuates ischemic brain injury although the underlying mechanisms have not been elucidated. Here we report that APC reinforces ischemia-reperfusion-induced mitophagy in middle cortical artery occlusion (MCAO)-treated mice, and in oxygen-glucose deprivation (OGD)-treated brain slices and neurons. Inhibition of mitophagy compromises neuroprotection conferred by APC. Furthermore, mitophagy and neuroprotection are abolished in Park2 knockout mice, indicating that APC-induced mitophagy is facilitated by the recruitment of PARK2 to mitochondria. Importantly, in MCAO mice, APC treatment extended the effective reperfusion window from 2 to 4 h, and this window was further extended to 6 h by exogenously expressing PARK2. Taken together, we found that PARK2-dependent APC-induced mitophagy renders the brain resistant to ischemic injury. APC treatment could be a favorable strategy to extend the thrombolytic time window for stroke therapy.

Berberine (BBR), a hypoglycemic agent, has shown beneficial metabolic effects for anti-diabetes, but its precise mechanism was unclear. Glucagon-like peptide-1 (GLP-1) is considered to be an important incretin that can decrease hyperglycemia in the gastrointestinal tract after meals. The aim of this study was to investigate whether BBR exerts its anti-diabetic effects via modulating GCG secretion. Diabeteslike rats induced by streptozotocin received BBR (120 mg/kg per day, i.g) for 5 weeks. Two hours following the last dose, the rats were anaesthetized and received 2 . 5 g/kg glucose by gavage. At 15-minute and 30-minute after glucose load, blood samples, pancreas, and intestines were obtained to measure insulin and GCG using ELISA kit. The number of L cells in the ileum and b-cells in the pancreas were identified using immunohistology. The expression of proglucagon mRNA in the ileum was measured by RT-PCR. The results indicated that BBR treatment significantly increased GCG levels in plasma and intestine (P!0 . 05) accompanied with the increase of proglucagon mRNA expression and the number of L-cell compared with the controls (P!0 . 05).Furthermore, BBR increased insulin levels in plasma and pancreas as well as b-cell number in pancreas. The data support the hypothesis that the anti-diabetic effects of BBR may partly result from enhancing GCG secretion.