Application of isotope dilution mass spectrometry: determination of ochratoxin A in the Canadian Total Diet Study

Tam, James P.; Pantazopoulos, Peter; Scott, Peter; Moisey, John; Dabeka, Robert; Richard, I.D.K.

doi:10.1080/19440049.2010.504750

Cited by 26 publications

(14 citation statements)

References 24 publications

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“…With an average content of 2.3 ng OTA per gram, raisins was found to be the most contaminated food item in the Canadian Total Diet Study (Tam et al 2011). In our study, reported intake of raisins was particularly associated with higher OTA exposure.…”

Section: Discussionmentioning

confidence: 43%

Mycotoxins in blood and urine of Swedish adolescents—possible associations to food intake and other background characteristics

et al. 2019

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The exposure to mycotoxins of Swedish adolescents is currently unknown. The aim of the present study was to investigate the exposure to mycotoxins and their association with food intake, and background characteristics in adolescents of a national dietary survey. About 3000 school students (1000 from the 5th, 8th and 11th school years) were recruited for the survey. The participants completed Web-based questionnaires on food propensity, sociodemography and health, and a Web-based dietary recall. Spot urine and blood samples were collected from 1105 of the participants for mycotoxin biomarker analysis. Mycotoxins were analysed with multibiomarker methods in urine (HPLC-MS/MS) and serum (HPLC-MS/MS). Of the 35 different analytes in urine, the frequency of positive samples were the following: deoxynivalenol (DON, 4.8%), DON-15-β-DO -glucuronide (DON-15GlcA, 9.1%), dihydro-citrinone (DH-CIT, 0.5%), HT-2-glucuronide (HT-2-3-GlcA, 0.1%) and ochratoxin A (OTA, 0.1%). Of the 27 different analytes in serum, OTA was detected in all samples, while 2'R-ochratoxin A (2'R-OTA) was found in 8.3% and enniatin B (EnB) in 99.2% of the samples. Exposure assessment calculations were performed on OTA from the serum concentration and on DON equivalents (DON eqv) from the urine concentration. All probable daily intake (PDI) estimates were below tolerable daily intakes, except for 1.6% of the participants for DON. The maximum PDI was 4.3 μg DON eqv/kg body weight and day. Consumption of cereal grain commodities was associated with levels of DON, EnB or OTA in biofluids. Serum OTAwas also associated with intakes of raisins and coffee. Furthermore, coffee consumption correlated well with 2'R-OTA concentration in serum. In conclusion, exposure to mycotoxins in Swedish adolescents is common, but fortunately, high exposure was rare.

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Section: Discussionmentioning

confidence: 43%

Mycotoxins in blood and urine of Swedish adolescents—possible associations to food intake and other background characteristics

et al. 2019

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“…In the Canadian Total Diet Study from 2008–2009 [ 69 ], samples of fresh pork meat, cured pork meat, canned luncheon meat, poultry meat, lamb meat, sausages, eggs and cheese were analyzed by LC-MS/MS. OTA was found only in the samples of fresh pork meat (OTA concentrations 0.03 and 0.23 ng/g), cured pork meat (OTA concentrations 0.06 and 0.20 ng/g) and sausages (OTA levels 0.12 and 0.06 ng/g).…”

Section: Discussionmentioning

confidence: 99%

Ochratoxin A Dietary Exposure of Ten Population Groups in the Czech Republic: Comparison with Data over the World

Ostrý

Malíř

Dofková

et al. 2015

Toxins

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Ochratoxin A is a nephrotoxic and renal carcinogenic mycotoxin and is a common contaminant of various food commodities. Eighty six kinds of foodstuffs (1032 food samples) were collected in 2011–2013. High-performance liquid chromatography with fluorescence detection was used for ochratoxin A determination. Limit of quantification of the method varied between 0.01–0.2 μg/kg depending on the food matrices. The most exposed population is children aged 4–6 years old. Globally for this group, the maximum ochratoxin A dietary exposure for “average consumer” was estimated at 3.3 ng/kg bw/day (lower bound, considering the analytical values below the limit of quantification as 0) and 3.9 ng/kg bw/day (middle bound, considering the analytical values below the limit of quantification as 1/2 limit of quantification). Important sources of exposure for this latter group include grain-based products, confectionery, meat products and fruit juice. The dietary intake for “high consumers” in the group 4–6 years old was estimated from grains and grain-based products at 19.8 ng/kg bw/day (middle bound), from tea at 12.0 ng/kg bw/day (middle bound) and from confectionery at 6.5 ng/kg bw/day (middle bound). For men aged 18–59 years old beer was the main contributor with an intake of 2.60 ng/kg bw/day (“high consumers”, middle bound). Tea and grain-based products were identified to be the main contributors for dietary exposure in women aged 18–59 years old. Coffee and wine were identified as a higher contributor of the OTA intake in the population group of women aged 18–59 years old compared to the other population groups.

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“…At present, RP HPLC is a method of choice for OTA determination in complex matrixes; nevertheless, many analysts prefer to store high concentration stock standard solutions in acidified non-polar solvents (benzene, toluene) matched by UV-spectrometry (ε 333 = 5440 [toluene-acetic acid] and ε 333 = 5600 [benzene-acetic acid] L*mol −1 *cm −1 ). To prepare calibrant solutions for RP-HPLC, the aliquot of stock standard solution is evaporated to dryness under stream of nitrogen at room temperature and redissolved in RP-HPLC suitable solvent [57][58][59][60].…”

Section: Ochratoxin Amentioning

confidence: 99%

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Kiseleva¹,

Chalyy²,

Седова³

et al. 2020

Toxins

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Standard solutions of mycotoxins prepared in RP HPLC solvents from neat standards are usually used for analytical method development. Multi-mycotoxin HPLC-MS/MS methods necessitate stability estimation for the wide spectrum of fungal metabolites. The stability of individual diluted stock standard solutions of mycotoxins in RP-HPLC solvents and multi-analyte HPLC-MS/MS calibrants was evaluated under standard storage and analysis conditions. Individual stock standard solutions of aflatoxins, sterigmatocystin, A-and B-trichothecenes, zearalenone and its analogues, ochratoxin A, fumonisins, Alternaria toxins, enniatins and beauvericin, moniliformin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin were prepared in RP-HPLC solvents and stored at −18 • C for 14 months. UV-spectroscopy was utilized to monitor the stability of analytes, excluding fumonisins. The gradual degradation of α-, β-zearalenol and α-, β-zearalanol in acetonitrile was detected. Aflatoxins and sterigmatocystin, zearalenone, Alternaria toxins, enniatins and beauvericin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin can be referred to as stable. The concentration of the majority of trichothecenes should be monitored. Diluted multi-mycotoxin standard in water/methanol (50/50 v/v) solutions acidified with 0.1% formic acid proved to be stable in silanized glass at 23 • C exposed to light for at least 75 h (CV ≤ 10%). An unexpected manifestation of MS/MS signal suppression/enhancement was discovered in the course of multi-mycotoxin standard solution stability evaluation. Key Contribution:The stability of the wide spectrum of mycotoxins in individual standard solutions in RP-HPLC solvents and multi-analyte HPLC-MS/MS calibrants under standard storage and analysis conditions is discussed basing on experimental and literature data.

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Application of isotope dilution mass spectrometry: determination of ochratoxin A in the Canadian Total Diet Study

Cited by 26 publications

References 24 publications

Mycotoxins in blood and urine of Swedish adolescents—possible associations to food intake and other background characteristics

Mycotoxins in blood and urine of Swedish adolescents—possible associations to food intake and other background characteristics

Ochratoxin A Dietary Exposure of Ten Population Groups in the Czech Republic: Comparison with Data over the World

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

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