Application of hyphenated analytical techniques in the investigation of selenium speciation of different plants

Abstract

The applicant met the requirements of the PhD regulations of the Corvinus University of Budapest and the thesis is accepted for the defence process.

Selenium Accumulation, Speciation and Localization in Brazil Nuts (Bertholletia excelsa H.B.K.)

“…The SeMet form, could either be present as a free amino acid or incorporated into proteins, which is also indistinguishable by μXANES. Studies [49,50,51], that used combinations of liquid chromatography and mass spectrometry, found the main form of Se in Brazil nuts to be SeMet. These studies detected this form after proteinase K treatment, suggesting that SeMet was incorporated into proteins.…”

“…Least-square linear combination fitting of the μXANES data was performed in the range of 12,630 to 12,850 eV using a library of 52 standard selenocompounds and procedures described by Fakra [54]. Additionally, a selenium valence scatter plot where kappa and mu represent the normalized absorption values at 12,664.25 and 12,667.8 eV respectively, was extracted from the Brazil nut μXANES data, using procedures described by Németh [51]. All data were recorded in fluorescence mode using a 7-elements Ge solid state detector (Canberra, ON) and processed using custom LabVIEW programs available at the beamline.…”