Application of Hydrolytic Enzymes in Biorefinery and Its Future Prospects

Kumar, Brijesh; Verma, Pradeep

doi:10.1007/978-981-15-7190-9_3

Cited by 22 publications

(2 citation statements)

References 109 publications

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“…Cellulase, amylase, glucoamylase, pectinase, and xylanase are key enzymes in biotechnology, each performing specific functions in the hydrolysis of biomass [18]. Cellulose is the most abundant biomaterial on Earth.…”

Section: Enzymesmentioning

confidence: 99%

Hydrogen Production from Enzymatic Pretreated Organic Waste with Thermotoga neapolitana

Tix,

Moll,

Krafft

et al. 2024

Energies

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Biomass from various types of organic waste was tested for possible use in hydrogen production. The composition consisted of lignified samples, green waste, and kitchen scraps such as fruit and vegetable peels and leftover food. For this purpose, the enzymatic pretreatment of organic waste with a combination of five different hydrolytic enzymes (cellulase, amylase, glucoamylase, pectinase and xylase) was investigated to determine its ability to produce hydrogen (H2) with the hydrolyzate produced here. In course, the anaerobic rod-shaped bacterium T. neapolitana was used for H2 production. First, the enzymes were investigated using different substrates in preliminary experiments. Subsequently, hydrolyses were carried out using different types of organic waste. In the hydrolysis carried out here for 48 h, an increase in glucose concentration of 481% was measured for waste loads containing starch, corresponding to a glucose concentration at the end of hydrolysis of 7.5 g·L−1. In the subsequent set fermentation in serum bottles, a H2 yield of 1.26 mmol H2 was obtained in the overhead space when Terrific Broth Medium with glucose and yeast extract (TBGY medium) was used. When hydrolyzed organic waste was used, even a H2 yield of 1.37 mmol could be achieved in the overhead space. In addition, a dedicated reactor system for the anaerobic fermentation of T. neapolitana to produce H2 was developed. The bioreactor developed here can ferment anaerobically with a very low loss of produced gas. Here, after 24 h, a hydrogen concentration of 83% could be measured in the overhead space.

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Section: Enzymesmentioning

confidence: 99%

Hydrogen Production from Enzymatic Pretreated Organic Waste with Thermotoga neapolitana

Tix,

Moll,

Krafft

et al. 2024

Energies

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“…Attention toward cellulase and other cellulolytic enzymes has considerably increased due to their importance in the hydrolysis of cellulosic materials to fermentable sugars to produce value-added products such as biofuel (Soares-Silva 2016 ; Kumar and Verma 2020b ). Some recent developments in the applications of cellulases are described below.…”

Section: The Industrial Perspective Of Cellulolytic Enzymesmentioning

confidence: 99%

Current perspective on production and applications of microbial cellulases: a review

Bhardwaj

Kumar

Agrawal

et al. 2021

Bioresour. Bioprocess.

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The potential of cellulolytic enzymes has been widely studied and explored for bioconversion processes and plays a key role in various industrial applications. Cellulase, a key enzyme for cellulose-rich waste feedstock-based biorefinery, has increasing demand in various industries, e.g., paper and pulp, juice clarification, etc. Also, there has been constant progress in developing new strategies to enhance its production, such as the application of waste feedstock as the substrate for the production of individual or enzyme cocktails, process parameters control, and genetic manipulations for enzyme production with enhanced yield, efficiency, and specificity. Further, an insight into immobilization techniques has also been presented for improved reusability of cellulase, a critical factor that controls the cost of the enzyme at an industrial scale. In addition, the review also gives an insight into the status of the significant application of cellulase in the industrial sector, with its techno-economic analysis for future applications. The present review gives a complete overview of current perspectives on the production of microbial cellulases as a promising tool to develop a sustainable and greener concept for industrial applications.

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Microbial Xylanases: A Helping Module for the Enzyme Biorefinery Platform

Bhardwaj

Verma

2021

Bioenergy Research

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Application of Hydrolytic Enzymes in Biorefinery and Its Future Prospects

Cited by 22 publications

References 109 publications

Hydrogen Production from Enzymatic Pretreated Organic Waste with Thermotoga neapolitana

Hydrogen Production from Enzymatic Pretreated Organic Waste with Thermotoga neapolitana

Current perspective on production and applications of microbial cellulases: a review

Microbial Xylanases: A Helping Module for the Enzyme Biorefinery Platform

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