Application of Hydrolase Catalytic Promiscuity in Organic Synthesis

Abstract

This review introduces the mechanism of hydrolase promiscuity. Several applications of hydrolase catalytic promiscuity in unit reaction, two-step reaction and three-component reaction are summarized, especially for addition, aldol reaction, and Knoevenagel reaction.

The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis

“…Further examples of non-natural conversions catalysed by promiscuous hydrolases can be found in a number of reviews. [240][241][242]…”

Synthesis of 2-Aminothiophene Derivatives Catalyzed by Amano Lipase M from Mucor javanicus

“…生物催化, 即以酶 [13] 或生物有机体 [14] 为催 化剂进行的化学转化过程, 酶作为绿色、高效、专一和 可生物降解的催化剂, 已广泛用于药物、农用化学品和 药物中间体 [15] 的合成中. 而研究者近来又发现, 酶不仅 有专一性 [16] , 还具有非专一性 [17] , 即酶可以催化传统反 应 外 多 种 其 它 的 有 机 反 应 , 如 脂 肪 酶 [18] 可 以 催 化 Mannich 反应 [19] 、Aldol 反应 [20] 和 Knoevenagel 反应 [21] , 蛋白酶可以催化 Michael 加成反应 [22] 、Henry 反应 [23] 和 Friedländer 反应 [24] 等. 出于对酶非专一性研究的持续关 注, 本文以活性腈和 2,5-二羟基-1,4-二噻烷为原料, 通 过爪哇毛霉脂肪酶催化的 Gewald 反应, 合成了一系列 2-氨基噻吩类化合物(Eq.…”

Synthesis and Antibacterial Activity of 2‐substitued‐(3‐pyridyl)‐quinazolinone Derivatives

“…It is an important intermediate in organic synthesis and drug molecular design . Pyridine compounds possess a variety of biological activities such as antibacterial , insecticidal , herbicidal , anticancer , anti‐neuropathic activities .…”