Application of High-Performance Liquid Chromatography to the Determination of Ascorbic Acid in Marine Plankton

Hapette, A. M.; Poulet, S. A.

doi:10.1080/01483919008049549

Cited by 18 publications

(8 citation statements)

References 20 publications

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“…lactis (L. leichmannii) ATCC 7830 (AOAC 2000). The content of vitamin C was determined by Shimadzu LC-6A HPLC system using a Develosil NH À5 2 Column (6×250 mm, Nomura Chemical ) and a RF550 spectrophotometer (Shimadzu) set for an excitation maximum at 355 nm and an emission maximum at 425 nm (Brown and Miller 1992) with samples extracted in a solution of 3% metaphosphoric acid and 8% acetic acid (MPA) at 4°C (Hapette and Poulet 1990).…”

Section: Methodsmentioning

confidence: 99%

Assessment of the nutritional value of Capsosiphon fulvescens (Chlorophyta): developing a new species of marine macroalgae for cultivation in Korea

2007

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The nutritive value of Capsosiphon fulvescens (C. Agardh) Setchell et Gardner, a new developing species for cultivation of marine macroalgae in Korea, was assessed by comparison with common edible green seaweed Ulva prolifera (Oeder) J. Agardh collected from Korea and Japan, based on analysis of its chemical composition. The contents of protein and of total, essential, and free amino acids of C. fulvescens were significantly higher than those of U. prolifera, whereas those of lipid, carbohydrate, and total dietary fibres were significantly lower. There were no significant differences in the moisture content between Capsosiphon and Ulva species. The main minerals of Capsosiphon and Ulva are Na, Mg, K, Ca, and Al, making up approximately 97-99 % of the total minerals. The contents of Na and V of Capsosiphon were significantly greater than those of Ulva, while those of Mn and Sr were significantly lower in Capsosiphon. The contents of retinal (27.8 μg g −1 dry wt) and ascorbic acid (0.28 mg g −1 dry wt) of Capsosiphon were significantly higher than those of Ulva, but the contents of cobalamin were lower. These results suggest that C. fulvescens has greater potential to be used as human food and as an ingredient in formulated food.

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Section: Methodsmentioning

confidence: 99%

Assessment of the nutritional value of Capsosiphon fulvescens (Chlorophyta): developing a new species of marine macroalgae for cultivation in Korea

2007

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“…At the end of the exponential phase of growth, each culture was harvested and triplicate samples of known volume (20-300 ml) were filtered on pre-ashed (45O"C, 8 h) GF/F filters and stored at -30°C or in liquid nitrogen (-172°C) for vitamin analysis. For each culture, samples were analyzed for Chl a (Lorenzen 1966), particulate organic C and N (with a Carlo Erba 1106 instrument standardized with cystine), total proteins (Bradford 1976), free amino acids (Roy and Poulet 1990), vitamin C (ascorbic and dehydroascorbic acids; Hapette and Poulet 1990;Poulet et al 1989), and fatty acids (Kattner and Fricke 1986).…”

Section: Methodsmentioning

confidence: 99%

Egg viability in the copepod Temora stylifera

Ianora

Poulet

1993

Limnology & Oceanography

133

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Estimates of production rate and viability of eggs are reported for Remora styIzj2ra collected in the Bay of Naples (Italy). Complementary observations on rates of fecal pellet and spcrmatophore production and female longevity were also conducted during the incubations. In situ egg viability was highly variable during the 2 yr of observation, with mean monthly values of ~80% of total egg production. Under experimental food-saturated conditions, the nature and hatching success of eggs were modified depending on type of food. With a dinoflagellate diet (Prorocentrum minimum), the responses of copepods were characterized by maximum production of viable eggs, high female longevity in the presence or absence of males, and low rates of fecal pellet production. The opposite was true with a diatom diet (Thalassiosira rot&z), except fecal pellets were produced at a higher rate, showing indirectly that this algae was neither properly assimilated nor transformed into production. Total and mean daily egg production were more or less the same with the two diets, indicating that under poorer food conditions copepods can maximize total egg production but not viability.

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“…Ecological transfer of ascorbate in the phytoplankton-zooplankton-fish food chain has been only fragmentarily examined (Dabrowski, 1991;Poulet et af., 1989). Phytoplankton is a rich source of ascorbate (Shigeoka et al, 1987) for herbivorous zooplankton, however, carnivorous zooplankters are already depleted in this vitamin due to transfer in the elongated food chain (Hapette & Poulet, 1990). Systematic studies into the presence of the enzyme that catalyses the last step of ascorbic acid synthesis, gulonolactone oxidase, are missing in aquatic animals.…”

Section: Introductionmentioning

confidence: 99%

Ascorbate concentration in fish ontogeny

Dąbrowski

1992

Journal of Fish Biology

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The ontogenetic trend of ascorbate has been quantified in three freshwater fishes: roach (Rurilus rufilus), whitefish (Coregonus lavarefus) and Arctic charr (Salvelinus ulpinus). Total ascorbate (reduced and oxidized) declined from 150 to 5 pg g-' as newly hatched larvae grew to become several-months-old juveniles. Declining total ascorbate with increasing size of metamorphosing fish could not be reversed by feeding on brine shrimp, Arfemiu salina nauplii, zooplanktonic food containing > 74 pg g I total ascorbate. The proportion of reduced ascorbate in total ascorbate increases with fish size/age. The physiological mechanism of the changes in transferable ascorbate forms remains unknown, but high dehydroascorbate concentrations suggest high vulnerability of larval fish to oxidation stress. This is the first report on quantity ofvitamin C retained inactivelyfeeding larval and juvenile fish. The efficiency of ascorbate transfer from zooplankters to larval fish amounted to 5-20%. The ecological significance of larval fish feeding on various zooplankters and/or phytoplankton may reflect a trend toward maximum transfer of this vitamin in freshwater food webs.

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Application of High-Performance Liquid Chromatography to the Determination of Ascorbic Acid in Marine Plankton

Cited by 18 publications

References 20 publications

Assessment of the nutritional value of Capsosiphon fulvescens (Chlorophyta): developing a new species of marine macroalgae for cultivation in Korea

Assessment of the nutritional value of Capsosiphon fulvescens (Chlorophyta): developing a new species of marine macroalgae for cultivation in Korea

Egg viability in the copepod Temora stylifera

Ascorbate concentration in fish ontogeny

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