Application of h-point standard addition method for simultaneous spectrophotometric determination of hydrochlorothiazide and propranolol

Abstract

A aplicação do método de adição padrão no ponto H para solucionar os espectros sobrepostos de hidroclorotiazida e propranolol foi avaliado. Os resultados mostram que o método HPSAM pode ser aplicado para a determinação simultânea dos compostos, em meio aquoso. Os resultados da aplicação do HPSAM mostraram que as duas drogas podem ser determinadas simultaneamente, numa razão de concentração hidroclorotiazida:propranolol variando entre 1:20 e 10:1 numa amostra sintética. O método foi aplicado com sucesso na determinação da hidroclorotiazida na presença de propranolol em algumas amostras sintéticas. Ainda, a aplicabilidade do método foi demonstrada pela determinação de ambos em alguns fluídos biológicos.The applicability of H-point standard addition method (HPSAM) to resolving overlapping spectra corresponding to the hydrochlorothiazide and propranolol was verified. The results show that the HPSAM is suitable for the simultaneous determination of hydrochlorothiazide and propranolol in aqueous media. The results of applying the HPSAM showed that the two drugs could be determined simultaneously with the concentration ratios hydrochlorothiazide:propranolol varying from 1:20 to 10:1 in a mixed sample. The proposed method has been successfully applied to the simultaneous determination of hydrochlorothiazide in the presence of propranolol in some synthetic samples. Moreover, the applicability of the method was demonstrated by their determination in some biological fluids.

The Spectrophotometric Multicomponent Analysis of a Ternary Mixture of Ibuprofen, Caffeine and Paracetamol by the Combination of Double Divisor‐Ratio Spectra Derivative and H‐Point Standard Addition Method

“…Where -CH is the unknown analyte concentration and S H is the analytical signal due to the interferent species. This method has been frequently applied to improve results in different analytical techniques, including spectrophotometry, [24][25][26][27][28][29] spectrofluorometry, 30 kinetic spectrophotometry [31][32][33] and chromatographic techniques. 34 However, there is no report on the application of HPSAM for analysis one component in ternary mixtures.…”

H-point Standard Addition Method for the Simultaneous Spectrophotometric Determination of Captopril and Hydrochlorothiazide in Pharmaceutical Formulations

“…A number of assay methods have been developed like voltammetric [12] , amperometric [13] and high sensitive nanomaterial-based voltammetric sensor [14] . Several H-point standard addition methods (HPSAMs) for the simultaneous spectrophotometric determination of hydrochlorothiazide and furosemide [15] , hydrochlorothiazide and propranolol [16] ,valsartan and hydrochlorothiazide [17] have been developed. Since UV-visible spectrophotometry is a fast, sensitive and low-cost analytical method, which is appropriate to monitor the dosage of pharmaceutical preparations.…”

Simultaneous spectrophotometric determination of phenolic antioxidant (BHA and BHT) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method

“…HPSAM transforms the incorrigible error to a constant systematic error, which can then be estimated and corrected. Previous studies highlight the high versatility and applicability of the method [26][27][28][29][30][31][32][33][34]. In this study, a detailed attempt has been made to simultaneously determine BHA and BHT using HPSAM in a simple, sensitive, and highly selective manner.…”