Application of Glutaraldehyde-Crosslinked Chitosan as a Scaffold for Hepatocyte Attachment.

Crosslinked chitosan—preparation and characterization

“…Such crosslinked chitosans might possibly exhibit specific biofunctionalities. 22,23 Finally, it should be noted that the crosslinking reaction catalyzed by potassium persulfate could possibly induce a conformational change in chitosan as observed by IR, X-ray diffraction and NMR studies. However, the influence of such a change on the properties of chitosan is not yet clearly understood and requires further investigation.…”

Releasing growth factors from activated human platelets after chitosan stimulation: a possible bio‐material for platelet‐rich plasma preparation

“…Chitosan has been suggested to be an excellent biomaterial (Rao & Sharma 1997;Muzzarelli et al 1999;Singla & Chawla 2001) and is used to promote cell attachment and growth (Klokkevold et al 1996;Anitua 1999;Lee et al 2000;Ishihara et al 2001;Zhao et al 2002;Mizuno et al 2003), and also as a carrier for the delivery of drugs or growth factors required to promote wound healing (Lee et al 2000). The positive attachment and growing abilities of the cells on chitosan has enabled the use of chitosan as a skeletal structure in tissue engineering for culturing hepatocytes, fibroblasts and cartilage cells (Guo et al 1989;Kawase et al 1997;Koyano et al 1998). Clot formation has been proposed to be one aspect of enhanced wound healing of chitosan based on the findings of a better neo-endothelial healing and a thin clot layer completely covering the implanted chitosan-impregnated vascular prostheses (Van der Lei & Wildevuur 1989;Van der Lei et al 1991).…”

Nanofiber-based delivery of bioactive agents and stem cells to bone sites

“…Microspheres are injectable and have been used as carriers to deliver cells for bone [101], cartilage [102], dermal [103], hepatic [104], and adipose [105] tissue regeneration. However, traditional microsphere fabrication techniques, such as emulsification [106] and emulsion polymerization [107], often lack control over the exterior and interior morphologies of the microspheres.…”