Releasing growth factors from activated human platelets after chitosan stimulation: a possible bio‐material for platelet‐rich plasma preparation

Shen, E-Chin; Chou, Tz-Chong; Gau, Ching-Hwa; Tu, Hsiao-Pei; Chen, Yen-Teen; Fu, Earl

doi:10.1111/j.1600-0501.2004.01241.x

Cited by 83 publications

(71 citation statements)

References 54 publications

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“…Platelet-derived growth factor concentrations have also been investigated by several other laboratories (Christgau et al, 2006;Cognasse et al, 2006;Doucet et al, 2005;Frechette et al, 2005;Gruber et al, 2002;Kaps et al, 2002;lacoste et al, 2003;Ogino et al, 2006;Schallmoser et al, 2007;Shen et al, 2006;Weibrich et al, 2002;Zimmermann et al, 2003). the results of these analyses are not comparable with our results, because of different preparation methods: All except el Backly et al and Gruber et al (el Backly et al, 2011;Gruber et al, 2002) stored platelets in serum, which also contains growth factors, and/or in platelet additive solution.…”

Section: Stimulation Of Erk1/2 Map Kinasescontrasting

confidence: 55%

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Rauch

2011

ALTEX

175

149

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Section: Stimulation Of Erk1/2 Map Kinasescontrasting

confidence: 55%

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Rauch

2011

ALTEX

175

149

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“…21 Cunha-Reis et al showed cell adhesion consistent with the nature of the HDD material used in this study. 22 In our study, sites receiving the HDD had improved postoperative healing with minimal complications when compared to the control site.…”

Section: Discussionsupporting

confidence: 86%

Effectiveness of Hemcon Dental Dressing Versus Conventional Method of Haemostasis in 40 Patients on Oral Antiplatelet Drugs

Kale¹,

Singh²,

Kotrashetti³

et al. 2012

SQUMJ

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abstract:Objectives: The purpose of the study was to evaluate the effectiveness of the HemCon Dental Dressing (HDD) in controlling post extraction bleeding and to ascertain its role in healing of extraction wounds, as compared to control. Methods: The 40 participants in the study were all receiving oral antiplatelet therapy (OAT). A total of 80 extractions were conducted without altering the patients' drug therapy. The extraction sites were divided into 2 groups: one group received a HDD, and the control group where the conventional method of pressure pack with sterile gauze under biting pressure (followed by suturing if required) was used to achieve haemostasis. Results: All HemCon treated sites achieved haemostasis sooner (mean = 53 seconds) than the control sites (mean = 918 seconds) which was statistically significant (P <0.001). Postoperative pain in the HDD group (1.74) was also significantly lower than in the control group (5.26) (P <0.001). Approximately 72.5% of HDD-treated sites showed significantly better postoperative healing when compared to the control site (P <0.001). Conclusion: HDD proved to be an excellent haemostatic agent that significantly shortened the bleeding time following dental extraction in patients on OAT. Additionally, HDD offered significantly improved post-operative healing of the extraction socket and less postoperative pain. Keywords

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“…The early hemostatic action of Axiostat Dental Dressing is by providing stable scaffold for RBCs to bind and aggregate under electrostatic attraction, and also to its adaptation within the socket providing frictional lock,under finger pressure. 10 The ADD is a chitosan-based material, which has got cell adhesion potential and is consistent according to Cunha-Reis et al 17 Shen et al 19 in their study stated that growth factors are released from human platelets stimulated by chitosan exposure, which can explain better healing observed with Axiostat Dressing. 20 Klokkevold et al 21 in their study stated that chitosan potentiates the differentiation of osteoprogenitor cells and may facilitate the formation of bone and help in healing, which can be attributed to superior healing in the study group.…”

Section: Discussionmentioning

confidence: 88%

Evaluating Effectiveness of Axiostat Hemostatic Material in achieving Hemostasis and Healing of Extraction Wounds in Patients on Oral Antiplatelet Drugs

Sharma¹,

Kale²,

Balihallimath³

et al. 2017

The Journal of Contemporary Dental Practice

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Aim:The aim of this study was to evaluate the efficacy of Axiostat Hemostatic Dental dressing in achieving hemostasis postextraction and determining its effect on pain and healing of the extraction wound, compared with control, i.e., conventional method of extraction in patients on oral antiplatelet therapy. Materials and methods:Totally, 40 patients on oral antiplatelet drugs were included in the study and overall 80 extractions were done applying split mouth study design, without altering patient's drug regime. Extraction sites were divided into two groups: Group I received Axiostat Hemostatic Dental Dressing (study site), and group II received conventional method; pressure pack with sterile gauze under biting pressure followed by suturing if required (control site) was used to attain hemostasis. Results: Extraction sites treated with Axiostat HemostaticDressing achieved hemostasis earlier (mean 1 minute 13 seconds) compared with control sites (mean = 14 minutes 1 second), which was also statistically significant (p < 0.001). Postoperative pain was considerably lower and significantly better healing was seen in the study group (p < 0.001) compared with the control. Conclusion:Axiostat demonstrated to be an effective hemostatic agent that considerably lessens the bleeding time in patients on oral antiplatelet drugs postextraction. In addition, it even offered minimal postoperative pain and improved healing of the extraction wound. On comparing the results of this study with our study on HemCon Dental Dressing, Axiostat Dental Dressing (ADD) is found to be as effective and at par in achieving hemostasis in patients on oral antiplatelet therapy. Clinical significance:The past few decades have seen an upsurge in use of low-dose aspirin either alone or in combination with other drugs. When these patients require dental/ maxillofacial treatment, earlier concept of stopping these medications is associated with increased risk of thromboembolic event. The present study highlights an alternative approach using ADD which aids in quick hemostasis, accentuates healing, and reduce postoperative pain.

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Releasing growth factors from activated human platelets after chitosan stimulation: a possible bio‐material for platelet‐rich plasma preparation

Abstract: Because of the observations of growth factors releasing from activated human platelets after chitosan stimulation, we suggest that chitosan may be an appropriate substitute for thrombin in PRP preparation.

Cited by 83 publications

References 54 publications

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Effectiveness of Hemcon Dental Dressing Versus Conventional Method of Haemostasis in 40 Patients on Oral Antiplatelet Drugs

Evaluating Effectiveness of Axiostat Hemostatic Material in achieving Hemostasis and Healing of Extraction Wounds in Patients on Oral Antiplatelet Drugs

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