Application of FTIR Method for the Assessment of Immobilization of Active Substances in the Matrix of Biomedical Materials

Kowalczuk, Dorota; Pitucha, Monika

doi:10.3390/ma12182972

Cited by 126 publications

(79 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ATR-FTIR spectroscopy has many uses in biopharmaceutical processing, some examples being the effective characterisation of biomaterials [64], the monitoring of monoclonal antibody purification [3], and biopharmaceutical bioactivity under stress conditions [65]. Other uses include the investigation of structural stability of biopharmaceuticals such as Bevacizumab® [65], Humatrope® [66], and Humalog® [67].…”

Section: In-linementioning

confidence: 99%

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

Tiernan

Byrne

Kazarian

2020

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

140

View full text Add to dashboard Cite

Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a label-free, non-destructive technique that can be applied to a vast range of biological applications, from imaging cancer tissues and live cells, to determining protein content and protein secondary structure composition. This review summarises the recent advances in applications of ATR-FTIR spectroscopy to biopharmaceuticals, the application of this technique to biosimilars, and the current uses of FTIR spectroscopy in biopharmaceutical production. We discuss the use of ATR-FTIR spectroscopic imaging to investigate biopharmaceuticals, and finally, give an outlook on the possible future developments and applications of ATR-FTIR spectroscopy and spectroscopic imaging to this field. Throughout the review comparisons will be made between FTIR spectroscopy and alternative analytical techniques, and areas will be identified where FTIR spectroscopy could perhaps offer a better alternative in future studies. This review focuses on the most recent advances in the field of using ATR-FTIR spectroscopy and spectroscopic imaging to characterise and evaluate biopharmaceuticals, both in industrial and academic research based environments.

show abstract

Section: In-linementioning

confidence: 99%

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

Tiernan

Byrne

Kazarian

2020

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

140

View full text Add to dashboard Cite

show abstract

“…To monitor changes in conformation, several methods have been implemented, including spectroscopy, as well as thermal and microscopy techniques [ 30 ]. For instance, through Fourier Transform Infrared (FTIR) spectroscopy, the conformational stability of immobilized enzymes has been estimated by deconvoluting the spectra of the immobilizates with the aid of the second derivative [ 31 , 32 ]. With this approach, it is possible to identify secondary structural changes induced by unfolding processes [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Congo Red Decolorization Using Textile Filters and Laccase-Based Nanocomposites in Continuous Flow Bioreactors

et al. 2020

View full text Add to dashboard Cite

Removal of azo and diazo dye content from textile industry wastewaters is crucial due to their environmental impact. Here, we report on the use of the fungal laccase from Pycnoporus sanguineus CS43 immobilized on silica nanoparticles and entrapped in textile-based filters for the degradation of Congo Red. Laccase immobilization and synthesis of the nanocomposites were carried out by two different methods, one in the presence of acetone and the second using water as solvent. This led to a change in the hydrophobicity of the obtained biofilters. Successful preparation of the nanocomposites was confirmed via FTIR spectroscopy. Changes in the secondary structure of the enzyme were inspected through the second derivative of the FTIR spectra. Six different types of filter were fabricated and tested in a continuous flow bioreactor in terms of their decolorization capabilities of Congo Red. The results indicate removal efficiencies that approached 40% for enzymes immobilized on the more hydrophobic supports. Backscattered electron (BSE) images of the different filters were obtained before and after the decolorization process. Percentage of decolorization and activity loss were determined as a function of time until a plateau in decolorization activity was reached. Experimental data was used to recreate the decolorization process in COMSOL Multiphysics® (Stockholm, Sweden). These simulations were used to determine the proper combination of parameters to maximize decolorization. Our findings suggest that the treatment of textile-based filters with immobilized laccase in conjunction with hydrophobic nanocomposites provides a suitable avenue to achieve more efficient laccase dye decolorization (39%) than that obtained with similar filters treated only with free laccase (8%). Filters treated with silica-based nanocomposites and immobilized laccases showed an increase in their decolorization capability, probably due to changes in their wetting phenomena.

show abstract

“…The use of FTIR technique has been proven quite significant to establish the efficient immobilisation of fungi in the corn cob. Studies on FTIR (Charumathi & Das, 2012;Kowalczuk & Pitucha, 2019) have been a guiding line and provide future prospects of studying site specific reactions can further support the study more efficiently. The simplicity of sample preparation, avoidance of chemical (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Confirmation of immobilization of fungal biomass on corn cobs by Fourier-Transform Infrared (FTIR) spectroscopy (Kowalczuk, 2019) Dried composite of fungal mycelium and mycelium induced corn cob, were macerated using sterilized mortar and pestle. Samples of 1 mg were mixed with 100 mg of spectroscopic grade KBr, HiMedia and analyzed against KBr background.…”

Section: Ementioning

confidence: 99%

Development, Optimization & Application of Corn Cobs as Cost Effective Matrix for Immobilization of Fungal Strains Paecilomyces sinensis and Geotrichum pseudocandidum

Gokhale¹,

Thakur²,

Deshpande³

2021

JSR

View full text Add to dashboard Cite

Fungal strains Paecilomyces sinensis and Geotrichum pseudocandidum were successfully immobilized on cheap yet effective support matrix, corn cobs. Immobilization was analysed by obtaining growth of the immobilized fungi from induced corn cob on solid media and further confirmed by FTIR analysis. Immobilization was successfully optimized on various temperatures and for different time duration. Activities of economic importance, performed by selected fungi were heavy metal absorption (in water and on solid medium), synthetic dye removal, enzyme production, were also performed efficiently by them in their immobilized form. Proposed work is an inclination towards immobilization of fungi via waste material appropriate for their growth and activity. Other fungi of economic importance may be immobilized using other matrices. The work suggests a cost-effective technique in order to increase industrial production.

show abstract

Application of FTIR Method for the Assessment of Immobilization of Active Substances in the Matrix of Biomedical Materials

Cited by 126 publications

References 59 publications

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

Congo Red Decolorization Using Textile Filters and Laccase-Based Nanocomposites in Continuous Flow Bioreactors

Development, Optimization & Application of Corn Cobs as Cost Effective Matrix for Immobilization of Fungal Strains Paecilomyces sinensis and Geotrichum pseudocandidum

Contact Info

Product

Resources

About