Application of first-derivative UV-spectrophotometry, TLC-densitometry and liquid chromatography for the simultaneous determination of mebeverine hydrochloride and sulpiride

Walily, Abdel Fattah M. El; Gindy, Alaa El; Bedair, Mohamed

doi:10.1016/s0731-7085(99)00176-4

Cited by 37 publications

(24 citation statements)

References 23 publications

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“…Several analytical methods have been used for SU determination including high performance liquid chromatography (HPLC) [2][3][4][5][6][7][8][9][10][11][12], thin layer chromatography (TLC) [13], capillary electrophoresis [14,15], flow injection, chemiluminescence [16], oscillopolarography [17], voltammetry [18], UV spectrophotometry [19][20][21], and fluorometry [22,23]. Two charge transfer spectrophotometric methods have been reported.…”

Section: Introductionmentioning

confidence: 99%

Two charge-transfer complex spectrophotometric methods for the determination of sulpiride in pharmaceutical formulations

Zayed

2009

Open Chemistry

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Two simple, rapid, accurate and sensitive spectrophotometric methods are described for the determination of sulpiride. They are based on charge transfer complexation between the drug as n-electron donor and p-chloranilic acid as π acceptor or iodine as σ-acceptor. These give highly coloured complexes with absorption maxima at 518 and 363, 294 nm, respectively. Beer's law linear ranges were 13.7 -341.4 and 1.7 -20.5 μg mL -1 for the p-chloranilic acid and iodine methods. The methods were successfully applied to the determination of the drug in Dogmatil ® Fort tablets and the results compared with the official method. The complex association constants and standard free energy changes were calculated using Benesi-Hildebrand plots. Warsaw and Springer-Verlag Berlin Heidelberg. © Versita

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Section: Introductionmentioning

confidence: 99%

Two charge-transfer complex spectrophotometric methods for the determination of sulpiride in pharmaceutical formulations

Zayed

2009

Open Chemistry

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“…A series of highly sensitive analytical methods have been developed for the determination of sulpiride, including gas chromatography (GC) with mass spectrometric 5,6 or oscillopolarographic detection, 7 high-performance liquid chromatography (HPLC) with ultraviolet, [8][9][10] fluorescence 11 or mass spectrometric detection, 12 capillary electrophoresis with electrochemiluminescence (CE-ECL), 2 cyclodextrin additives 3 or ultraviolet. 13 Unfortunately, all of these measurement techniques are time-consuming and may be complicated by the need for tedious pretreatment procedures, i.e., sample extraction in the routinely used HPLC methods [8][9][10][11][12] or CE-ECL analysis.…”

Section: Introductionmentioning

confidence: 99%

“…13 Unfortunately, all of these measurement techniques are time-consuming and may be complicated by the need for tedious pretreatment procedures, i.e., sample extraction in the routinely used HPLC methods [8][9][10][11][12] or CE-ECL analysis. 2 It is well recognized that spectrofluorometric methods can produce high sensitivity and clean spectrum acquisition, and are suitable for the analysis of drugs that possess natural fluorescence, such as sulpiride.…”

Section: Introductionmentioning

confidence: 99%

Determination of Sulpiride in Human Urine Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration

et al. 2007

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“…11 A literature survey also reveals several methods for assaying SU, involving HPLC, 4,[12][13][14][15] TLC, 16,17 fluorometric, 18 spectrophotometric, 19,20 flow injection chemiluminometric, 21 capillary electrophoresis, 22 oscillopolarographic, 23 and adsorptive stripping voltammetric 24 methods. El-Walily et al 25 first used derivative UV spectrophotometry, TLC-densitometry and HPLC for the simultaneous determination of both of these two compounds in pharmaceutical tablets. In this study, ratio spectra first derivative spectrophotometry and classical least squares method are proposed for simultaneous determination of MB and SU in their mixtures and their pharmaceutical preparation, Colona tablets.…”

Section: Introductionmentioning

confidence: 99%

“…Several analytical methods have been used for the determination of MB, including high performance liquid chromatography (HPLC), 2-4 thin layer chromatography (TLC), 5,6 spectrophotometric methods 25 first used derivative UV spectrophotometry, TLC-densitometry and HPLC for the simultaneous determination of both of these two compounds in pharmaceutical tablets. In this study, ratio spectra first derivative spectrophotometry and classical least squares method are proposed for simultaneous determination of MB and SU in their mixtures and their pharmaceutical preparation, Colona tablets.…”

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confidence: 99%

Simultaneous Determination of Mebeverine Hydrochloride and Sulpiride Using the First Derivatives of Ratio Spectra and Chemometric Methods

Zayed

2005

ANAL. SCI.

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Mebeverine hydrochloride (MB), 4-[ethyl(4-methoxy-α-methylphenethyl)amino]butyl veratrate hydrochloride [2753-45-9], is a potent direct antispasmodic acting mainly on the smooth muscles of the gastrointestinal tract; it is particularly effective against colonic spasms. 1 Sulpiride (SU), N-(1-ethylpyrrolidin-2-ylmethyl)-2-methoxy-5-sulfamoyl benzamide [15676-16-1], is a substituted benzamide antipsychotic which is reported to be a selective antagonist of central dopamine (D2, D3 and D4) receptors. It is also claimed to have mood-elevating properties. 1The binary mixture of the two drugs is used in the treatment of gastointestinal and colic spasms, specially in the presence of psychasomatic manifestations of nervous tension, mental stress or anxiety. Several analytical methods have been used for the determination of MB, including high performance liquid chromatography (HPLC), 2-4 thin layer chromatography (TLC), 5,6 spectrophotometric methods 25 first used derivative UV spectrophotometry, TLC-densitometry and HPLC for the simultaneous determination of both of these two compounds in pharmaceutical tablets. In this study, ratio spectra first derivative spectrophotometry and classical least squares method are proposed for simultaneous determination of MB and SU in their mixtures and their pharmaceutical preparation, Colona tablets. The proposed ratio spectra first derivative method was found to be more sensitive than the published UV first derivative method 25 (Beer's law linear range was 4.0 -40.0 and 1.0 -10.0 µg/ml for MB and SU, respectively while the linear range was 10.0 -30.0 and 2.0 -8.0 µg/ml in the first derivative published method). The proposed chemometric method was more rapid, simple than the published methods. 25 Also, the proposed methods can be used as alternative methods to those reported for the simultaneous determination of MB and SU. Experimental ApparatusA Shimadzu 1601 double beam UV-Vis spectrophotometer with a fixed slit width (2 nm) connected to an IBM compatible computer and a HP 600 inkjet printer was used. The bundled software was UV PC personal spectroscopy version 3.91 (Shimadzu). MaterialsMB, SU and Colona tablets containing 100 mg MB and 25 mg SU per tablet, were kindly supplied by Rameda (6 October City, Egypt). Analytical grade methanol and hydrochloric acid were used throughout these experiments. Standard solutionsStock solutions of MB and of SU were prepared separately by dissolving 100 mg and 10 mg of each drug, respectively, in 100 ml methanol. The standard solutions were prepared by dilution of the stock solutions with 0.1 M HCl to reach a concentration range of 4 -20 and 1 -10 µg/ml for MB and SU, respectively. Faculty of Industrial Education, Beni Suef, EgyptTwo methods are described for the simultaneous determination of mebeverine hydrochloride (MB) and sulpiride (SU) in their combinations. The first method depends on the first derivative of the ratio spectra by measurement of the amplitudes at 263.7 and 234.9 nm for MB and SU, respectively. The linear ranges and detection...

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Application of first-derivative UV-spectrophotometry, TLC-densitometry and liquid chromatography for the simultaneous determination of mebeverine hydrochloride and sulpiride

Cited by 37 publications

References 23 publications

Two charge-transfer complex spectrophotometric methods for the determination of sulpiride in pharmaceutical formulations

Two charge-transfer complex spectrophotometric methods for the determination of sulpiride in pharmaceutical formulations

Determination of Sulpiride in Human Urine Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration

Simultaneous Determination of Mebeverine Hydrochloride and Sulpiride Using the First Derivatives of Ratio Spectra and Chemometric Methods

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