Application of Filter Paper Partition Chromatography to Qualitative Analysis of Volatile and Non-Volatile Organic Acids.

Fink, Kay; Fink, Robert M.

doi:10.3181/00379727-70-17024

Cited by 94 publications

(21 citation statements)

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“…A control incubation and one with the addition of 0 5 % urea was carried out in each case. Volatile organic acids in the products of incubation were estimated semi-quantitatively by the method of Fink and Fink (1949). Table I gives the results of the control experiment on six rabbits.…”

Section: Methodsmentioning

confidence: 99%

Caecal pH and ammonia in experimental uraemia.

Bourke¹,

Milne²,

Stokes³

1966

Gut

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EDITORIAL SYNOPSIS This paper describes the effect on the pH, ammonia, and urea content of the caecum in rabbits made uraemic by bilateral nephrectomy. The considerable quantities of free ammonia present are considered in relation to uraemic colitis in man.Urea is freely diffusible from the blood into the lumen of the alimentary tract, where it is converted by the enzyme urease into ammonia and CO2. Tracer studies indicate that at least 25 % of the urea in the body undergoes hydrolysis within the gut (Walser and Bodenlos, 1959), but a proportion of the ammonia produced is absorbed and re-synthesized to urea in the liver. The practical importance of this metabolic cycle, therefore, lies in the concentration of ammonia within the gut and its effect on the pH of the gastric and intestinal fluids.

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Section: Methodsmentioning

confidence: 99%

Caecal pH and ammonia in experimental uraemia.

Bourke¹,

Milne²,

Stokes³

1966

Gut

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“…This conclusion was supported by 13 C NMR and DEPT spectra. The compound was proved to be phenol from its positive phosphomolybedic acid test (Seher and Seifen, 1959), ferric chloride test (Fink and Fink, 1949). It was recognized to be flavone as it responded to Shinoda test (Grayer, 1989).…”

Section: Resultsmentioning

confidence: 98%

Constituents of Pterocarpus marsupium: an ayurvedic crude drug

et al. 2004

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“…TLC was carried out using Merck precoated silica gel plates. Hydroxamic acids were located on TLC plates by spraying 1.25% FeCl 3 in 1 N HCl (8). Thioesters were visualized by spraying p-anisaldehyde-acetic acid-ethanol-concentrated H 2 SO 4 (9.3:3.8:340:12.5, vol/vol) and heating at 100°C for 10 min (27).…”

Section: Methodsmentioning

confidence: 99%

Novel Pathway for Utilization of Cyclopropanecarboxylate by Rhodococcus rhodochrous

Toraya

Oka

Ando

et al. 2004

Appl Environ Microbiol

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A new strain isolated from soil utilizes cyclopropanecarboxylate as the sole source of carbon and energy and was identified as Rhodococcus rhodochrous (H. Nishihara, Y. Ochi, H. Nakano, M. Ando, and T. Toraya, J. Ferment. Bioeng. 80:400-402, 1995). A novel pathway for the utilization of cyclopropanecarboxylate, a highly strained compound, by this bacterium was investigated. Cyclopropanecarboxylate-dependent reduction of NAD ؉ in cell extracts of cyclopropanecarboxylate-grown cells was observed. When intermediates accumulated in vitro in the absence of NAD ؉ were trapped as hydroxamic acids by reaction with hydroxylamine, cyclopropanecarboxohydroxamic acid and 3-hydroxybutyrohydroxamic acid were formed. Cyclopropanecarboxyl-coenzyme A (CoA), 3-hydroxybutyryl-CoA, and crotonyl-CoA were oxidized with NAD ؉ in cell extracts, whereas methacrylyl-CoA and 3-hydroxyisobutyryl-CoA were not. When both CoA and ATP were added, organic acids corresponding to the former three CoA thioesters were also oxidized in vitro by NAD ؉ , while methacrylate, 3-hydroxyisobutyrate, and 2-hydroxybutyrate were not. Therefore, it was concluded that cyclopropanecarboxylate undergoes oxidative degradation through cyclopropanecarboxyl-CoA and 3-hydroxybutyryl-CoA. The enzymes catalyzing formation and ring opening of cyclopropanecarboxyl-CoA were shown to be inducible, while other enzymes involved in the degradation were constitutive.

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Application of Filter Paper Partition Chromatography to Qualitative Analysis of Volatile and Non-Volatile Organic Acids.

Cited by 94 publications

References 0 publications

Caecal pH and ammonia in experimental uraemia.

Caecal pH and ammonia in experimental uraemia.

Constituents of Pterocarpus marsupium: an ayurvedic crude drug

Novel Pathway for Utilization of Cyclopropanecarboxylate by Rhodococcus rhodochrous

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