Novel Pathway for Utilization of Cyclopropanecarboxylate by
            <i>Rhodococcus rhodochrous</i>

Toraya, Tetsuo; Oka, Takayuki; Ando, Masayoshi; Yamanishi, Mamoru; Nishihara, Hiroshi

doi:10.1128/aem.70.1.224-228.2004

Cited by 9 publications

(4 citation statements)

References 30 publications

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“…and the inducible nature of these enzymes ( Blakley, 1978 ). Almost nothing is known about the CPCA metabolic intermediates, while a study on the use of cyclopropanecarboxylate (CPrCA) by R. rhodochrous indicated that CPrCA is initially converted into its CoA thioester which undergoes the ring-opening reaction in order to be further degraded through the β-oxidation pathway ( Toraya et al, 2004 ). In the present study, the chcpca cluster, coding for enzymes involved in β-oxidation reactions, is transcriptionally induced in BCP1 cells utilizing CPCA and CHCA as the only carbon and energy source.…”

Section: Discussionmentioning

confidence: 99%

Aerobic Growth of Rhodococcus aetherivorans BCP1 Using Selected Naphthenic Acids as the Sole Carbon and Energy Sources

et al. 2018

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Naphthenic acids (NAs) are an important group of toxic organic compounds naturally occurring in hydrocarbon deposits. This work shows that Rhodococcus aetherivorans BCP1 cells not only utilize a mixture of eight different NAs (8XNAs) for growth but they are also capable of marked degradation of two model NAs, cyclohexanecarboxylic acid (CHCA) and cyclopentanecarboxylic acid (CPCA) when supplied at concentrations from 50 to 500 mgL-1. The growth curves of BCP1 on 8XNAs, CHCA, and CPCA showed an initial lag phase not present in growth on glucose, which presumably was related to the toxic effects of NAs on the cell membrane permeability. BCP1 cell adaptation responses that allowed survival on NAs included changes in cell morphology, production of intracellular bodies and changes in fatty acid composition. Transmission electron microscopy (TEM) analysis of BCP1 cells grown on CHCA or CPCA showed a slight reduction in the cell size, the production of EPS-like material and intracellular electron-transparent and electron-dense inclusion bodies. The electron-transparent inclusions increased in the amount and size in NA-grown BCP1 cells under nitrogen limiting conditions and contained storage lipids as suggested by cell staining with the lipophilic Nile Blue A dye. Lipidomic analyses revealed significant changes with increases of methyl-branched (MBFA) and polyunsaturated fatty acids (PUFA) examining the fatty acid composition of NAs-growing BCP1 cells. PUFA biosynthesis is not usual in bacteria and, together with MBFA, can influence structural and functional processes with resulting effects on cell vitality. Finally, through the use of RT (Reverse Transcription)-qPCR, a gene cluster (chcpca) was found to be transcriptionally induced during the growth on CHCA and CPCA. Based on the expression and bioinformatics results, the predicted products of the chcpca gene cluster are proposed to be involved in aerobic NA degradation in R. aetherivorans BCP1. This study provides first insights into the genetic and metabolic mechanisms allowing a Rhodococcus strain to aerobically degrade NAs.

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Section: Discussionmentioning

confidence: 99%

Aerobic Growth of Rhodococcus aetherivorans BCP1 Using Selected Naphthenic Acids as the Sole Carbon and Energy Sources

et al. 2018

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“…Furthermore, sesquiterpene pyridine alkaloids from Tripterygium were predicted to target several proteins and signaling pathways and may play an important role in curing Alzheimer’s disease, Chagas disease, and NAFL ( Long et al, 2021 ). The aminobenzoate degradation pathway could promote tryptophan metabolism and benzoate degradation ( Naidu and Ragsdale, 2001 ; McLeish et al, 2003 ; Toraya et al, 2004 ). The animal studies by Zhang et al showed that the main changes in fecal metabolites of high-fat fed mice included metabolites related to tryptophan metabolism ( Zhang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Revealing the Mechanism of Huazhi Rougan Granule in the Treatment of Nonalcoholic Fatty Liver Through Intestinal Flora Based on 16S rRNA, Metagenomic Sequencing and Network Pharmacology

et al. 2022

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Background: The incidence of Nonalcoholic Fatty Liver (NAFL) is increasing year by year, growing evidence suggests that the intestinal flora plays a causative role in NAFL. Huazhi Rougan Granule (HRG) is commonly used in the clinical treatment of NAFL. It is reported that it can reduce lipids and protect the liver, but no research has confirmed whether the drug’s effect is related to the intestinal flora. Therefore, we investigated whether the effect of HRG is related to the regulation of intestinal flora to further explore the mechanism of HRG in the treatment of NAFL through intestinal flora.Methods: In this study, C57BL/6J mice were fed a high-fat diet for 8 weeks, and the high-fat diet plus HRG or polyene phosphatidylcholine capsules were each administered by gavage for 4 weeks. High-throughput sequencing, network pharmacology, and molecular docking were used to explore the mechanism of HRG in the treatment of NAFL through intestinal flora.Results: HRG treatment can reduce body weight gain, lipid accumulation in liver and lipogenesis and reduce serum biochemical indexes in high-fat-fed mice. Analysis of intestinal flora showed that HRG changed the composition of intestinal flora, which was characterized by a decrease in the Firmicutes/Bacteroidetes ratio. Moreover, the species distribution was significantly correlated with AKP, HDL-C, and TG. Metagenetic analysis showed that HRG altered the functional composition and functional diversity of microorganisms, which was mainly characterized by an increase in the abundance of metabolic pathways. The network pharmacology results show that the mechanism of HRG in the treatment of NAFL through intestinal flora is mainly reflected in the biological process of gene function and related to infectious diseases, immune systems, and signal transduction pathways, such as cytokine-cytokine receptor interaction, Chagas disease, IL-17 signaling pathway and other signaling pathways.Conclusion: These results strongly suggest that HRG may alleviate NAFL by preventing IFD.

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“…KEGG analysis comparing OTA and OTA-Se groups revealed that Nano-Se could influence aminobenzoate and benzoate degradation by elevating 4-Hydroxybenzoic Acid, Protocatechuic Acid levels. This aminobenzoate degradation pathway may promote tryptophan metabolism and benzoate breakdown ( 94 ). Protocatechuic acid, a primary metabolite of complex polyphenols, exhibits numerous biological activities, encompassing antioxidant, anti-inflammatory, antibacterial, and antiapoptotic properties ( 95 – 97 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of nano-selenium on cecum microbial community and metabolomics in chickens challenged with Ochratoxin A

Fang,

Hu,

Liu

2023

Front. Vet. Sci.

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IntroductionOchratoxin A (OTA) is a widely distributed mycotoxin. Nano-selenium (Nano-Se) is an emerging form of selenium known for its superior bioavailability, remarkable catalytic efficiency, and robust adsorbing capacity. Despite these characteristics, its impact on the microbial community and metabolomics in the cecum of chickens exposed to OTA has been infrequently investigated. This research examined the microbiota and metabolomic alterations linked to OTA in chickens, with or without Nano-Se present.MethodsA cohort of 80 healthy chickens at the age of 1 day was randomly distributed into four groups of equal numbers, namely the Se cohort (1 mg/kg Nano-Se), the OTA cohort (50 μg/kg OTA), the OTA-Se cohort (50 μg/kg OTA + 1 mg/kg Nano-Se), and the control group. Each chicken group’s caecal microbiome and metabolome were characterized using 16S rRNA sequencing and Liquid chromatography coupled with mass spectrometry (LC–MS) analyses.Results and discussionOur results showed that the on day 21, the final body weight was significantly reduced in response to OTA treatments (p < 0.05), the average daily gain in the OTA group was found to be inferior to the other groups (p < 0.01). In addition, Nano-Se supplementation could reduce the jejunum and liver pathological injuries caused by OTA exposure. The 16S rRNA sequencing suggest that Nano-Se supplementation in OTA-exposed chickens mitigated gut microbiota imbalances by promoting beneficial microbiota and suppressing detrimental bacteria. Moreover, untargeted metabolomics revealed a significant difference in caecal metabolites by Nano-Se pretreatment. Collectively, the dataset outcomes highlighted that Nano-Se augmentation regulates intestinal microbiota and associated metabolite profiles, thus influencing critical metabolic pathways, and points to a possible food-additive product.

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Novel Pathway for Utilization of Cyclopropanecarboxylate by Rhodococcus rhodochrous

Cited by 9 publications

References 30 publications

Aerobic Growth of Rhodococcus aetherivorans BCP1 Using Selected Naphthenic Acids as the Sole Carbon and Energy Sources

Aerobic Growth of Rhodococcus aetherivorans BCP1 Using Selected Naphthenic Acids as the Sole Carbon and Energy Sources

Revealing the Mechanism of Huazhi Rougan Granule in the Treatment of Nonalcoholic Fatty Liver Through Intestinal Flora Based on 16S rRNA, Metagenomic Sequencing and Network Pharmacology

Effects of nano-selenium on cecum microbial community and metabolomics in chickens challenged with Ochratoxin A

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