Application of Evidence Investigator<sup>TM</sup>for the Simultaneous Measurement of Soluble Adhesion Molecules: L‐, P‐, E‐ Selectins, VCAM‐1, ICAM‐1 in a Biochip Platform

McAleer, Damien; McPhillips, Frances M.; Fitzgerald, S.; McConnell, R.I.; Rodriguez, Maria‐Luz

doi:10.1080/15321810600862298

Cited by 12 publications

(9 citation statements)

References 32 publications

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“…The reproducibility and long-term stability are essential for measurements in the multicomponent field using new technologies like biochip array The system turned out to be easy to handle and very stable over a long period of time with mean coefficients of variation of around 10% which is compa rable with those published earlier for the Cytokine and Growth Factors array (2) and recently for the Cell Adhesion Molecule array (8).…”

Section: Resultssupporting

confidence: 52%

Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Pelt¹,

Romijn²

2009

Journal of Medical Biochemistry

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Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence InvestigatorMulti component assays are a promising development in laboratory medicine. Reproducibility and standardization of the used technology is crucial for the quality of the results. In our laboratory several studies were carried out in a period of more than two years using the Randox Evidence Investigator. We applied the Cytokine & Growth Factors and Cell Adhesion Molecule Array from which reference values could be obtained. Cytokines are not useful in low grade inflammation or in normal persons as the levels are too low to detect. However growth factors and cell adhesion molecules could be determined in those studies. Outcomes with the arrays were compared with conventional assays such as ELISA. The correlations of IL-6 and IL-10 were very good and that of s-ICAM acceptable. Inter-assay coefficients of variation could be calculated by using the same control level material during those years. The system turned out to be easy to handle and very stable over a long period of time with CV's of about 8-12%. The results obtained are not dependent of the lot number of the arrays or apparatus as two different apparatus gave same outcomes.

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Section: Resultssupporting

confidence: 52%

Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Pelt¹,

Romijn²

2009

Journal of Medical Biochemistry

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“…Biochip array technology enables simultaneous detection of multiple cytokines and adhesion molecules in a single sample and provides valuable information relating to each tested analyte and possible associations between analytes in each sample 16,17 . We previously tested single cytokines and adhesion molecules in leukemia patients.…”

Section: Discussionmentioning

confidence: 99%

Multi-analytical evaluation of serum levels of cytokines and adhesion molecules in patients treated for acute myeloid leukemia using biochip array technology

Horáček¹,

Vašatová²,

Kupsa³

et al. 2013

BIOMED PAP

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Aims. Evaluation of serum levels of 17 cytokines and 5 adhesion molecules in patients treated for acute myeloid leukemia (AML) using biochip array technology. This approach allows multi-analytical determination from a single sample. Methods. A total of 15 AML patients were studied. Blood samples were taken at the diagnosis (active leukemia) and at circa 6 months after completion of last chemotherapy (durable complete remission in all patients).Results. Comparing cytokine and adhesion molecule levels in active leukemia and in durable complete remission, we found significant increase (P<0.01) in serum interleukin-7 (IL-7), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and significant decrease (P<0.01) in serum E-selectin. Discussion. Our results indicate that serum levels of specific cytokines and adhesion molecules (IL-7, EGF, VEGF, E-selectin) are significantly altered in patients treated for AML, reflecting activity of the disease. Further investigation is needed to establish if the changes observed in the levels of these molecules could be used as a prognostic indicator of AML.

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“…The light signal generated from each of the test regions on the biochip is detected using digital imaging technology and compared to that from a stored calibration curve. The concentration of analyte present in the sample is calculated from the calibration curve 17,18 .…”

Section: Multi-analytical Evaluation Using Biochip Array Technologymentioning

confidence: 99%

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

Horáček¹,

Kupsa²,

Vašatová³

et al. 2015

BIOMED PAP

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Aims.To compare serum levels of 17 cytokines and 5 adhesion molecules in patients with newly diagnosed acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) using biochip array technology. Methods. A total of 15 AML and 15 ALL patients were studied. Serum samples were taken prior to anticancer therapy and were analyzed by biochip based immunoassays on the Evidence Investigator analyzer. This approach allows simultaneous detection of multiple analytes from a single sample. T-tests were used for statistical analysis. Results. Comparing cytokine and adhesion molecules levels in newly diagnosed AML and ALL patients, we found significant increase in AML in serum IL-4 (P < 0.0001), IL-2 (P < 0.01), IL-3 (P < 0.05), and significant decrease (P < 0.05) in serum VEGF and VCAM-1. Discussion. Our results indicate that serum profile of cytokines and adhesion molecules differs in newly diagnosed AML and ALL patients. Further studies are needed to establish if these alterations could be used as a clinically relevant biomarker for acute leukemias.

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Application of Evidence Investigator^TMfor the Simultaneous Measurement of Soluble Adhesion Molecules: L‐, P‐, E‐ Selectins, VCAM‐1, ICAM‐1 in a Biochip Platform

Cited by 12 publications

References 32 publications

Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Multi-analytical evaluation of serum levels of cytokines and adhesion molecules in patients treated for acute myeloid leukemia using biochip array technology

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

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Application of Evidence InvestigatorTMfor the Simultaneous Measurement of Soluble Adhesion Molecules: L‐, P‐, E‐ Selectins, VCAM‐1, ICAM‐1 in a Biochip Platform

Cited by 12 publications

References 32 publications

Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Multi-analytical evaluation of serum levels of cytokines and adhesion molecules in patients treated for acute myeloid leukemia using biochip array technology

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

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Product

Resources

About

Application of Evidence Investigator^TMfor the Simultaneous Measurement of Soluble Adhesion Molecules: L‐, P‐, E‐ Selectins, VCAM‐1, ICAM‐1 in a Biochip Platform