Application of ELISA in determining the fate of tetracyclines in land-applied livestock wastes

Abstract

The potential use of a class-specific enzyme-linked immunosorbent assay (ELISA) in studying the occurrence and fate of tetracyclines in the environment was evaluated. Several manure samples collected from hog lagoons and cattle feedlots were screened for the presence of tetracycline residues using ELISA. The levels varied from less than the detection limit (0.5 parts per billion) to 200 parts per million. The degradation of tetracyclines in soil-applied manure was followed using ELISA to measure the decline in tetracycline concentrations. Low levels of tetracyclines remained detectable in soil for up to 28 days. The ELISA procedure also proved useful in determining the leaching potential of tetracyclines in undisturbed soil columns and in the analysis of total tetracyclines in manure, soil, and water. Based on the cross-reactivity of the antibodies employed, this ELISA method can be an important screening tool for the presence of other tetracycline compounds, such as chlortetracycline and oxytetracycline. The ELISA method also detects the epimers of tetracyclines and the corresponding dehydration by-products, anhydrotetracyclines. Analysis of selected manure extracts by liquid chromatography with mass spectrometry (LC-MS) showed lower concentrations of total tetracyclines compared to the values obtained by ELISA, indicating the presence of other structurally related compounds or transformation products of tetracyclines being detected by ELISA in the samples. Because analysis of manure and soil samples by LC-MS requires extensive clean-up procedures, ELISA provides an alternative method for conducting environmental fate and transport studies of antibiotics.

Development of Simultaneous Analytical Method of Veterinary Antibiotics in Manure using Liquid Chromatography Coupled with Tandem Mass Spectrometry

“…( · ) (HallingSorensen et al, 2002;Aga et al, 2003, Vaclavik et al, 2004. , · , , (Choi et al, 2008;Lim et al, 2009;Lee et al, 2010;Ok et al, 2011;Kim et al, 2012;Lim et al ., 2014).…”

Validation of the exposure assessment for veterinary medicinal products

“…Screening level models do not intend to represent reality accurately, but to provide rapid predictions of the potential environmental fate of a compound (Tarazona et al, 2003). To validate this claim, the available screening models are compared in Section 2 to field data published in literature for soil, surface water and groundwater (Hamscher et al, 2000, Hamscher et al, 2002, Winckler and Grafe, 2001b, Boxall et al, 2002, Schlüsener et al, 2003, Aga et al, 2003and De Liguoro et al, 2003.…”

Detection of Oxytetracycline in Broiler Chicken Meat Marketed in Several Cities in Java Island Using Enzyme-Linked Immunosorbent Assay (Elisa) Method

“…However, overuse and insufficient lengths of withdrawal of OTC antibiotic in animal husbandry may result in the presence of residues in animal products (Centikaya et al, 2012) that may endanger human health and even lead to its presence in the environment (Aga et al, 2003). For the purpose of monitoring and ensuring food safety of consumers to the dangers of residues in food products, CODEX (CAC, 2012) sets the maximum residue limits (MRL) for tetracycline (CTC, TC and OTC) as the sum of parent compounds and its metabolites in poultry products which are 200 ng/g for meat, 600 ng/g for liver and 400 ng/g for eggs.…”