Application of Electrochemical Techniques for Determining and Extracting Natural Product (EgCg) by the Synthesized Conductive Polymer Electrode (Ppy/Pan/rGO) Impregnated with Nano-Particles of TiO2

Abstract

The polypyrrole/polyaniline-based electrode (Ppy/Pan/TiO 2 /rGO) was fabricated via the electrophoretic deposition technique on fluorine-doped tin oxide (FTO)-coated glass. Physico-electrochemical adsorption/desorption of epigallocatechin gallate (EgCg) as an electroactive species was enhanced by the fabricated electrode compared to the electroless technique extraction using the same electrode. EgCg was electrochemically extracted using chronoamperometry by electrophoretically deposited Ppy/Pan/TiO 2 /rGO film. Isolated EgCg was qualified and quantified by the voltammetry and high-performance liquid chromatography (HPLC) techniques. It was found that the extracted EgCg values were 3.38 and 0.72 ppm from a 10 ppm prepared solution using the electrochemically and physically based techniques, respectively. Morphology/ elemental analysis and crystal structure of the prepared electrodes were characterized by field emission scanning electron microscopy/energy-dispersive X-ray (FESEM/EDX) and X-ray diffraction (XRD), respectively. The conductivity of the fabricated electrode was investigated by electrochemical impedance spectroscopy (EIS) and was calculated as 1.124 S/cm for the electrophoretically deposited electrodes (EPD).

Phytochemical Composition, Antioxidant Activity, and Enzyme Inhibitory Activities (α-Glucosidase, Xanthine Oxidase, and Acetylcholinesterase) of Musella lasiocarpa

“…Moreover, the epiatechin derivative is known as a bioactive molecule with anticancer, anti-oxidant, hepatoprotective, anti-inflammatory, and anti-microbial properties [ 29 ]. Overall, the compounds identified were mainly flavonoids, which may be responsible for the excellent antioxidant activities and α-glucosidase inhibitory activity of the EtOAc fraction.…”

Electrochemical Profiling of Plants

“…These non-conducting ingredients consume key parts of plant tissues, making it difficult to analyze the conducting counterpart ingredients. An embedding plant strategy has been designed to ensure selective and amplified responses using a matrix or polymers [25,110]. It is a relatively tedious job to summarize the protocols and resolve the varieties and species domains in the Plantae kingdom; hence, conventional systems are not applicable in real-world analysis.…”