Application of Electrochemical Biosensors in Clinical Diagnosis

Monošík, Rastislav; Stredansky, Matuš; Šturdı́k, Ernest

doi:10.1002/jcla.20500

Cited by 85 publications

(31 citation statements)

References 106 publications

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“…A typical electrochemical DNA sensor involves a solid electrode such as mercury, gold, and carbon electrodes which could generate corresponding electrochemical signals based on interactive binding between probe and hybridized or covalently tagged DNA element [33]. Carbon based nanomaterials such as carbon nanotube, pristine graphene or graphene oxide has been experimentally employed recently as the sensing platform [34].…”

Section: Carbon Based Materials As Dna Sensormentioning

confidence: 99%

Graphene Sculpturene Nanopores for DNA Nucleobase Sensing

et al. 2014

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To demonstrate the potential of nanopores in bilayer graphene for DNA sequencing, we computed the current-voltage characteristics of a bilayer graphene junction containing a nanopore and found that they change significantly when nucleobases are transported through the pore. To demonstrate the sensitivity and selectivity of example devices, we computed the probability distribution PX(β) of the quantity β representing the change in the logarithmic current through the pore due to the presence of a nucleobase X (X = adenine, thymine, guanine, or cytosine). We quantified the selectivity of the bilayer-graphene nanopores by showing that PX(β) exhibits distinct peaks for each base X. To demonstrate that such discriminating sensing is a general feature of bilayer nanopores, the well-separated positions of these peaks were shown to be present for different pores, with alternative examples of electrical contacts.

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Section: Carbon Based Materials As Dna Sensormentioning

confidence: 99%

Graphene Sculpturene Nanopores for DNA Nucleobase Sensing

et al. 2014

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“…Novel biosensing devices which aim for the high performance detection of different natural metabolites are of great relevance [1].…”

Section: Introductionmentioning

confidence: 99%

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Taurino

Reiss

Richter

et al. 2013

Electrochimica Acta

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Keywords:l-Lactate oxidases from Aerococcus viridans Engineered and commercial enzymes Multi-walled carbon nanotubes Enzyme biosensor a b s t r a c t A comparison between engineered and commercially available l-lactate oxidases from Aerococcus viridans was conducted for biosensing applications. Enzymes were adsorbed onto the surfaces of graphite electrodes modified with multi-walled carbon nanotubes. Thermostable l-lactate oxidases were cloned with a (i) N-, (ii) a C-terminal His-tag and (iii) a wild-type enzyme. Subsequently to the heterologous expression in Escherichia coli and purification, we determined the kinetic parameters of these enzymes in solution. The kinetics of the wild-type, of the N-terminally His-tagged enzyme and of the commercial l-lactate oxidase from A. viridans were studied with a classical Michaelis-Menten as well as with a substrate inhibition model, while the enzyme carrying a C-terminal His-tag showed no activity. The active enzymes were used to fabricate and comparatively investigate multi-walled carbon nanotubes-based biosensors. The enzyme kinetic results were compared with electrochemical studies. By using both spectrophotometric and amperometric techniques, the inhibition phenomenon fits better to the data especially those data related with Lox-His-N. The electrochemical data of the fabricated enzymatic biosensors showed that the N-terminally His-tagged l-lactate oxidase performed best on carboxyl-modified carbon nanotubes. The sensor based on this engineered enzyme showed the highest sensitivity and lowest detection limit in the range of l-lactate concentration 0-1 mM as well as long term stability over one month.

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“…First enzyme based glucosensor (Clark & Lyons, 1962) used glucose oxidase to measure glucose by utilizing oxygen electrode that is placed on the surface of semipermeable dialysis membrane to quantify changing redox state during the biochemical reaction. In fact, this strategy was modernized to develop various types of enzyme-based biosensor to monitor various substances such as cholesterol, lactate and urea in biological fluids (Abdelwahab, Won, & Shim, 2010;Alqasaimeh, Heng, & Ahmad, 2007;Alqasaimeh, Heng, Ahmad, Raj, & Ling, 2014;Gruhl, Rapp, & Lange, 2013;Monošȋk, Stred'anský, & Šturdik, 2012). The applications are even extended for toxicity analysis in environmental monitoring, food safety and quality control areas in combination with optics and microbial fuel cell (Amine, Mohammadi, Bourais, & Palleschi, 2006;Long, Zhu, & Shi, 2013;Sun et al, 2015).…”

Section: Electrochemistry and Biosensorsmentioning

confidence: 99%

Current technological trends in biosensors, nanoparticle devices and biolabels: Hi‐tech network sensing applications

Vigneshvar

Senthilkumaran

2018

Med Devices & Sens

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Biosensors are micro‐analytical devices usually coupled to a bio‐integrated transducer that emits signal to measure specific molecules as an analyte, either qualitatively or quantitatively for various downstream applications. Improved detection methods at quantum level in nanoscale became a reality due to the invention of integrated circuits involving nanoparticle devices and biolabels. Next level of discoveries leads to the identification of novel particulates to improve the specificity and sensitivity for single analyte detection. Although different kinds of biosensors show promise for multifaceted applications in science and technology, certain limitations do exist in terms of portability, selectivity and sensitivity as well as in multi‐analyte detection. Recent methodical advancements involving nanomaterials or nanoparticles/metals in combination with biomarkers or biolabels as sensors provide new insights to solve these limitations to prepare flawless detecting devices. These technical advances are evident in modern‐day sensors including hand‐held, smartphone‐based, wearable and flexible sensors developed for multifaceted applications. This concise review article describes the new technological trends in the development of biosensors integrated to nanoparticle devices and/or biolabels involving hi‐tech network system for better efficiency. Future perspectives on new technological developments for effective combination of several nanomaterials and biolabels with strategic engineering methodology were highlighted.

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Application of Electrochemical Biosensors in Clinical Diagnosis

Cited by 85 publications

References 106 publications

Graphene Sculpturene Nanopores for DNA Nucleobase Sensing

Graphene Sculpturene Nanopores for DNA Nucleobase Sensing

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Current technological trends in biosensors, nanoparticle devices and biolabels: Hi‐tech network sensing applications

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