Application of Electro-energizing Method to<scp>l</scp>-Glutamic Acid Fermentation

Hongo, Motoyoshi; Iwahara, M.

doi:10.1080/00021369.1979.10863776

Cited by 27 publications

(28 citation statements)

References 5 publications

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“…Among the developing Microbial Electrochemical Technologies (METs), the use of redox mediators to transport electrons to or from microorganisms has received considerable attention due to its potential to uncouple energy metabolism from growth (Clark et al, 2012) and to alter terminal metabolite production toward more valuable products during fermentation (Emde and Schink, 1990; Hongo and Iwahara, 1979; T. Kim and B. Kim, 1988; S.…”

Section: Introductionmentioning

confidence: 99%

“…M. Park et al, 2005; Peguin et al, 1994) in a process that has been called both “microbial electrosynthesis” (Patil et al, 2015; Schröder et al, 2015), and “electro-fermentation” (Rabaey and Rozendal, 2010). The delivery of electrons to fermenting cells for biocommodity production has been successfully implemented using several different mediators, including neutral red, benzyl viologen, methyl viologen, humic substances, and anthraquinone 2,6-disulfonate (Emde and Schink, 1990; Hongo and Iwahara, 1979; T. Kim and B. Kim, 1988; S.…”

Section: Introductionmentioning

confidence: 99%

“…M. Park et al, 2005; Peguin et al, 1994). Among these, neutral red has shown promise due to its low toxicity and low standard reduction potential (E o = −525 mV Ag/AgCl ) (Hongo and Iwahara, 1979; D. H. Park and Zeikus, 1999).…”

Section: Introductionmentioning

confidence: 99%

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The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction

Harrington

Tran

Mohamed

et al. 2015

Bioresource Technology

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The aim of this work was to elucidate the mechanism of mediated microbial electrosynthesis via neutral red from an electrode to fermenting Escherichia coli cultures in a bioelectrochemical system. Chemical reduction of NAD+ by reduced neutral red did not occur as predicted. Instead, neutral red was shown to reduce the menaquinone pool in the inner bacterial membrane. The reduced menaquinone pool altered fermentative metabolite production via the arcB redoxsensing cascade in the absence of terminal electron acceptors. When the acceptors DMSO, fumarate, or nitrate were provided, as many as 19% of the electrons trapped in the reduced acceptors were derived from the electrode. These results demonstrate the mechanism of neutral red-mediated microbial electrosynthesis during fermentation as well as how neutral red enables microbial electrosynthesis of reduced terminal electron acceptors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction

Harrington

Tran

Mohamed

et al. 2015

Bioresource Technology

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“…Among the mediators employed, which include benzyl and methyl viologen (Peguin and Soucaille, 1996; Rao and Mutharasan, 1986, 1987), anthraquinone disulfate (AQDS) (Clark et al, 2012; Emde and Schink, 1990; Sasaki et al, 2013), and neutral red (Girbal et al, 2006; Hongo and Iwahara, 1979; Park and Zeikus, 1999; Park et al, 2005), the latter has shown promise because it does not form a radical, undergoes two-electron reduction and oxidation (Azariah et al, 1998), binds to the cell membrane and is less toxic (Park and Zeikus, 1999), and has a standard reduction potential below that of the NADH/NAD + couple (−525 mV Ag / AgCl vs. −520 mV Ag / AgCl ) (McKinlay and Zeikus, 2004). It has been known for over 100 years that certain bacteria biochemically reduce neutral red anaerobically.…”

Section: Introductionmentioning

confidence: 99%

Neutral red-mediated microbial electrosynthesis by Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis

Harrington

Mohamed

Tran

et al. 2015

Bioresource Technology

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The aim of this work was to compare the effects of electrosynthesis on different bacterial species. The effects of neutral red-mediated electrosynthesis on the metabolite profiles of three microorganisms: Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis, were measured and compared and contrasted. A statistically comprehensive analysis of neutral red-mediated electrosynthesis is presented using the analysis of end-product profiles, current delivered, and changes in cellular protein expression. K. pneumoniae displayed the most dramatic response to electrosynthesis of the three bacteria, producing 93% more ethanol and 76% more lactate vs. control fermentation with no neutral red and no electron delivery. Z. mobilis showed no response to electrosynthesis except elevated acetate titers. Stoichiometric comparison showed that NAD+ reduction by neutral red could not account for changes in metabolites during electrosynthesis. Neutral red-mediated electrosynthesis was shown to have multifarious effects on the three species.

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“…In addition, considering an energy-efficient bioprocess in common, electrochemically driven fermentation could provide more opportunities to create different redox balancing, directing myriads of metabolic routes for generation of diverse product(s) (Hongo and Iwahara 1979;Rabaey and Rozendal 2010;Rabaey et al 2011). Regarding electron-transport-assisted bioprocesses, Rabaey and Rozendal (2010) first mentioned the term "electrofermentation. "…”

Section: Introductionmentioning

confidence: 99%

Electron transport phenomena of electroactive bacteria in microbial fuel cells: a review of Proteus hauseri

Hsueh

Chen

2017

Bioresour. Bioprocess.

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This review tended to decipher the expression of electron transfer capability (e.g., biofilm formation, electron shuttles, swarming motility, dye decolorization, bioelectricity generation) to microbial fuel cells (MFCs). As mixed culture were known to perform better than pure microbial cultures for optimal expression of electrochemically stable activities to pollutant degradation and bioenergy recycling, Proteus hauseri isolated as a "keystone species" to maintain such ecologically stable potential for power generation in MFCs was characterized. P. hauseri expressed outstanding performance of electron transfer (ET)-associated characteristics [e.g., reductive decolorization (RD) and bioelectricity generation (BG)] for electrochemically steered bioremediation even though it is not a nanowire-generating bacterium. This review tended to uncover taxonomic classification, genetic or genomic characteristics, enzymatic functions, and bioelectricity-generating capabilities of Proteus spp. with perspectives for electrochemical practicability. As a matter of fact, using MFCs as a tool to evaluate ET capabilities, dye decolorizer(s) could clearly express excellent performance of simultaneous bioelectricity generation and reductive decolorization (SBG and RD) due to feedback catalysis of residual decolorized metabolites (DMs) as electron shuttles (ESs). Moreover, the presence of reduced intermediates of nitroaromatics or DMs as ESs could synergistically augment efficiency of reductive decolorization and power generation. With swarming mobility, P. hauseri could own significant biofilm-forming capability to sustain ecologically stable consortia for RD and BG. This mini-review evidently provided lost episodes of great significance about bioenergysteered applications in myriads of fields (e.g., biodegradation, biorefinery, and electro-fermentation). Keywords

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Application of Electro-energizing Method tol-Glutamic Acid Fermentation

Cited by 27 publications

References 5 publications

The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction

The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction

Neutral red-mediated microbial electrosynthesis by Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis

Electron transport phenomena of electroactive bacteria in microbial fuel cells: a review of Proteus hauseri

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