Application of doubled haploid technology in breeding of Brassica napus

Burbulis, Natalija; Kott, L. S.

doi:10.1533/9781908818478.183

Cited by 3 publications

(1 citation statement)

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“…Likewise, high oleic acid-containing Brassica was also achieved (Turner and Facciotti 1990;Wong and Swanson 1991). Then, a similar approach was used to locate lower glucosinolate containing B. napus germplasm (Burbulis et al 2001). Afterward, cold tolerant, salt-tolerant, and sclerotia-resistant mutant lines have developed through this In vitro mutagenesis approach (Rahman et al 1995;Liu et al 2005;McClinchey and Kott 2008).…”

Section: Brassica Perspectivesmentioning

confidence: 99%

Anther Culture in Crop Plants: Progress and Perspectives

Islam

Arif

Hasan

et al. 2023

Plant Breed. Biotech.

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A resurrection has started in haploid and double haploid research in the twenty-first century. The haploid and double haploid could be achieved through in vivo and in vitro anther and microspore culture techniques. Fixing the homozygosity is the most striking benefit of androgenesis. Various factors like genotypic dependency, growth condition, developmental stage of the microspore, pre-treatment, culture media, regeneration media, growth hormones, and various chemicals have a direct effect. Wheat, rice, Brassica, and tobacco are the notable crops where anther and microspore culture has been utilized. These haploidy and double haploidy through anther culture served many purposes of basic and applied research. Especially, double haploid cultivars have been cultivating around the globe. In addition, for chromosome mapping, QTL mapping, marker-assisted selection, marker-assisted backcrossing, mutation breeding, genome-wide association study, genomic engineering, and genome editing, androgenesis based haploid and double haploid plants have been exploited due to the effectiveness. Recently, researchers are trying to explain albinism that happens during anther culture from an epigenetic perspective. Further prospects of haploid and doubled haploid research through anther culture have been described in this review.

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Section: Brassica Perspectivesmentioning

confidence: 99%

Anther Culture in Crop Plants: Progress and Perspectives

Islam

Arif

Hasan

et al. 2023

Plant Breed. Biotech.

View full text Add to dashboard Cite

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Combined Effect of Biochar and Plant Growth-Promoting Rhizbacteria on Physiological Responses of Canola (Brassica napus L.) Subjected to Drought Stress

Gul-Lalay,

Ullah,

Shah

et al. 2024

J Plant Growth Regul

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Biochar (BC) and plant growth-promoting microbes (PGPR) could represent a suitable agronomical strategy to mitigate the impacts of drought in arid agro-environmental conditions. However, there is currently little understanding of the synergistic benefit of combining BC and PGPR to increase drought tolerance in oilseeds. In this study, the physiological response of two water-stressed canola (Brassica napus L.) plants subjected to the application of BC obtained from waste wood of Morus alba applied solely or in combination with PGPR strains (Pseudomonas sp.) was evaluated. The experiment consists of two genotypes and nine treatments [(C-Control, T1-15 days drought (15DD), T2-30 days drought (30DD), T3-15 days of drought + PG (15DD + PG), T4-30 days of drought + PG (30DD + PG), T5-15 days drought + biochar (15DD + BC), T6-30 days drought + biochar (30DD + BC), T7-15 days drought + biochar + PG (15DD + BC + PG), T8-30 days drought + biochar + PG (30DD + BC + PG)]. Drought stress decreased emergence energy (EE), leaf area index (LAI), leaf area ratio (LAR), root shoot ratio (RSR), moisture content of leaves (MCL), percent moisture content (%MC), moisture content of shoot (MCS) and moisture content of root (MCR), and relative water content (RWC) in both varieties of Brassica napus L., which in contrast, it is increased by the collective application of both biochar and PGPR. In both varieties, N, P, K, Mg, and Ca concentrations were highest in all the biochar and PGPRs separate and combined treatments, while lowest in 15 and 30 days drought treatments. Osmolyte contents like Glycine betaine (GB) and sugar remarkably increased in the stress condition and then reduced due to the synergistic application of biochar and PGPR. Drought stress has a repressive effect on the antioxidant enzymatic system like Peroxidase (POD), Superoxide dismutase (SOD), and glutathione reductase (GR) as well as total flavonoids, phenolics, and protein content. The antioxidant enzymes and phenolic compounds were dramatically increased by the combined action of biochar and PGPRs. A significant increase in EE, LAR, RSR, and RWC under 15 and 30 days drought conditions, evidently highlighting the synergistic effect of BC and PGPR. The results conclude a substantial and positive effect of the combined use of BC and PGPR strains on canola's response to induced drought stress, by regulating the physiological, biochemical, and agronomic traits of the plants. Graphical Abstract

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