Application of DNA aptamers as sensing layers for detection of carbofuran by electrogenerated chemiluminescence energy transfer

Carbon-based dots for electrochemiluminescence sensing

“…Aptamer-based biosensors have attracted much attention due to the versatile target and low cost of aptamers, 127 so Li et al fabricated an aptamer sensor for carbofuran detection on the basis of the ECL energy transfer between fullerene C60-loaded Au nanoparticles and CDs. 128 After specifically binding with the DNA aptamer, carbofuran blocked energy transfer and decreased the ECL intensity because the change of the tertiary structure of the DNA aptamer by binding the target caused the CDs labeled on the aptamer to move far away from the electrode surface. The decreasing ECL signal showed a linear correlation with the carbofuran concentration, thereby establishing a new method for quantitative analysis.…”

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

“…C 60 -AuNPs act as energy donor while carbon dot acts as receptor to create electrogenerated chemiluminescence energy transfer (ECRET) for the detection of carbofuran. The presence of carbofuran in test sample induces structural change in aptamer to block the chemiluminescence [ 23 ]. Phorate, an organophosphate pesticide, can selectively induce aggregation of ssDNA aptamer-AgNPs.…”

“…QDs, iron oxide, magnesium oxide, and manganese oxide NPs have been used as optical nanosensor and can also be explored to design DNA nanosensor for large number of unexplored analytes as an alternative to routine complex analytical assays. Luminescence, chemiluminescence and phosphoresce abilities of nanomaterials are still neglected and not explored using existing nanomaterials and available DNA sequences [ 23 , 24 ]. Change in the properties of intercalating fluorescent dyes or electroactive substances in presence and absence of analyte leading to change in florescence or electrochemical signal also has great scope.…”

What are the Main Sensor Methods for Quantifying Pesticides in Agricultural Activities? A Review

“…This method has an LOD of 8.8 10 × 13 mol L −1 . This same method was developed for the insecticide, ChP, and the nematicide, CBY, for the aqueous samples, with an LOD of 24 μg/mL [79].…”