Application of Dispersion Staining to the Study of Refractive Index of Dental Enamel

Wiemann, M.R.; Besic, F.C.; Keller, O.

doi:10.1177/00220345700490011401

J Dent Res

1970

DOI: 10.1177/00220345700490011401

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Application of Dispersion Staining to the Study of Refractive Index of Dental Enamel

M.R. Wiemann¹,

F.C. Besic²,

O. Keller³

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“…Historically, this parameter was used to determine purity of the enamel and dentin powders [Manly et al, 1939]. Conventional measurement of n by dispersion staining required immersion of the dental substance in fluids with matching n [Wiemann et al, 1970]. An emerging class of n measurement methods employs low-coherence interferometry, a principle on which OCT is based.…”

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Estimation of the Enamel and Dentin Mineral Content from the Refractive Index

et al. 2012

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Recent advances in the field of optics have enabled accurate and localized measurement of optical properties of biological substrates. This work aimed to elucidate the relationship between the local refractive index (n) and mineral content (MC) of enamel and dentin. De- and remineralized lesions in bovine enamel and dentin blocks were sectioned into 300- to 400-µm-thick slices, and placed on a metal plate to capture images of sound, de- and remineralized regions transversely by optical coherence tomography. Mean n at each depth level of the lesion (20- or 40-µm steps for enamel or dentin) was measured by the optical path length-matching method and used to plot n through lesion depth. The specimens were further polished and processed for transverse microradiography for analysis of MC. The n and MC ranged from 1.52 to 1.63 and 50 to 87 (vol.%) in enamel, and from 1.43 to 1.57 and 11 to 48 (vol.%) in dentin, respectively. Strong, positive linear correlations were found between n and MC (Pearson’s r = 0.95 and 0.91 for de- and remineralized enamel, and r = 0.94 and 0.91 for dentin, respectively, p < 0.001). Experimental data were validated with a theoretical calculation of n from MC. De- and remineralization of enamel and dentin resulted in measurable changes of n, and, in turn, MC changes of the tissue could be estimated with good accuracy from this long-known optical property by the new analytical approach. Compositional changes of enamel crystallites after remineralization affect n.

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Estimation of the Enamel and Dentin Mineral Content from the Refractive Index

et al. 2012

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The effect of ultraviolet induced fluorescence on visually perceived tooth color under normal light conditions

Hein¹,

Bosch

2018

Dental Materials

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Dispersion Staining, Dispersion, and Refractive Indices in Early Enamel Caries

Besic

Wiemann

1972

J Dent Res

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Application of Dispersion Staining to the Study of Refractive Index of Dental Enamel

Cited by 3 publications

References 0 publications

Estimation of the Enamel and Dentin Mineral Content from the Refractive Index

Estimation of the Enamel and Dentin Mineral Content from the Refractive Index

The effect of ultraviolet induced fluorescence on visually perceived tooth color under normal light conditions

Dispersion Staining, Dispersion, and Refractive Indices in Early Enamel Caries

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