Application of different spectrofluorimetric methods for determination of lesinurad and allopurinol in pharmaceutical preparation and human plasma

Attia, Khalid A.M.; El‐Olemy, Ahmed; Ramzy, Sherif; Hasan, M. A.; Omar, Mahmoud K.M.; Shahin, Mohamed Y

doi:10.1016/j.saa.2020.118871

Cited by 18 publications

(8 citation statements)

References 11 publications

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“…[ 37 ] It also can be used to quantitatively determine several medicines in a single run due to its small, precise, and sharp spectrum. [ 36 , 38 , 39 , 40 , 41 ]…”

Section: Introductionmentioning

confidence: 99%

“…[37] It also can be used to quantitatively determine several medicines in a single run due to its small, precise, and sharp spectrum. [36,[38][39][40][41] To date, no method for the simultaneous quantification of FPV and HCQ as co-administered drugs in biological fluids has been documented. This necessitates the establishment of a responsive F I G U R E 1 Chemical structure of (a) favipiravir and (b) hydroxychloroquine sulphate procedure for simultaneous estimation of both drugs for their therapeutic drug monitoring.…”

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confidence: 99%

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Quantitative analysis of favipiravir and hydroxychloroquine as FDA‐approved drugs for treatment of COVID‐19 using synchronous spectrofluorimetry: application to pharmaceutical formulations and biological fluids

et al. 2022

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Coronavirus disease 2019 (COVID‐19) is a contagious viral infection caused by coronavirus 2 (SARS‐CoV‐2) that causes severe acute respiratory syndrome. It has ravaged several countries and burdened many healthcare systems. As the process of authorizing a novel treatment for human use is extensive and involves multiple phases to obtain safety information and identify potential concerns. Therefore, the fastest and easiest choice was to use United States Food and Drug Administration (US FDA)‐approved drugs such as favipiravir and hydroxychloroquine. For the simultaneous estimation of both medications, a simple synchronous spectrofluorimetric approach was established in which both drugs were measured at 372 and 323 nm, respectively in the presence of each other without interference at Δλ 60 nm. The effect of various experimental conditions on synchronous fluorescence intensities were thoroughly investigated and optimized. The maximum synchronous fluorescence intensities were obtained at pH 5.4 using acetate buffer (0.2 M, 0.5 ml) and ethanol as a diluent. Excellent linearity ranges were obtained using 1.0–18.0 ng/ml and 10.0–120.0 ng/ml for favipiravir and hydroxychloroquine, respectively. The approach exhibited high sensitivity with detection limits down to 0.25 ng/ml and 1.52 ng/ml and quantitation limits down to 0.77 ng/ml and 4.62 ng/ml, respectively. Spiking human plasma samples with the studied drugs yielded high % recoveries, allowing a significant bioanalytical application. Moreover, the method was validated according to International Conference on Harmonization guidelines and further applied to commercial pharmaceutical preparations with good results.

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“…[ 37 ] It also can be used to quantitatively determine several medicines in a single run due to its small, precise, and sharp spectrum. [ 36 , 38 , 39 , 40 , 41 ]…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Quantitative analysis of favipiravir and hydroxychloroquine as FDA‐approved drugs for treatment of COVID‐19 using synchronous spectrofluorimetry: application to pharmaceutical formulations and biological fluids

et al. 2022

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“…The authors also established the robustness of the developed method by using constancy of the fluorescence intensity with small variations in the optimum conditions such as pH (±0.2) and buffer volume (±0.2 mL) of LSD and ALLP in addition to 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) volume (±0.2 mL) and heating temperature (±10°C) for ALLP only. It was confirmed that there was no considerable effect on the fluorescence intensity of LSD and ALLP resulted from minor changes of procedure parameters [ 24 ]. All of the UV-visible and spectrofluorimetric methods of ALLP are summarized in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

A Brief Review of Analytical Methods for the Estimation of Allopurinol in Pharmaceutical Formulation and Biological Matrices

Sharma

Sapkota

Dangi

2021

International Journal of Analytical Chemistry

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This review article represents the collection and discussion of various analytical methods available in the literature for the determination of allopurinol (ALLP) in pharmaceutical and biological samples consisting of HPLC, UV-visible method, near-IR spectroscopy, spectrofluorometry, capillary electrophoresis, polarography, voltammetry, and hyphenated techniques such as LC-MS, LC-MS/MS, UPLC-MS/MS, and GC-MS. The anticipated review provides details about the comparative utilization of various analytical techniques for the determination of ALLP. The present review article can be effectively explored to conduct future analytical investigation for the estimation of ALLP.

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“…This overlaying difficulty might be confronted by the employing synchronous technique, as it has several of benefits over standard fluorescence techniques, which include high selectivity, proper spectra, and minimum intrusion [34][35][36] . SFS is highly basic but proved to be an effective approach for the quantitative assessment of the two drugs in an appropriate time frame because of its narrow and sharp spectra [37][38][39][40] . REM and SIM emit separable synchronous…”

Section: Simultaneous Spectrofluorimetic Determination Of Remdesivir ...mentioning

confidence: 99%

Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma

Sharkasy

Tolba

Belal

et al. 2022

Sci Rep

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As new infectious mutations of SARS-CoV-2 emerged throughout the world, innovative therapies to counter the virus-altered drug sensitivities were urgently needed. Several antiviral options have been in clinical trials or in compassionate use for the treatment of SARS-CoV-2 infections in an attempt to minimize both clinical severity and viral shedding. Recent research indicated that simeprevir acts synergistically with remdesivir, allowing for a multiple-fold decrease in its effective dose when used at physiologically acceptable concentrations. The goal of this work is to develop a sensitive synchronous spectrofluorimetric approach to simultaneously quantify the two drugs in biological fluids. Using this method, remdesivir and simeprevir could be measured spectrofluorimetrically at 283 and 341 nm, respectively, without interference from each other using Δλ of 90 nm. The effect of various experimental parameters on the fluorescence intensity of the two drugs was extensively explored and optimized. For each of remdesivir and simeprevir, the method exhibited a linearity range of 0.10–1.10 μg/mL, with lower detection limits of 0.01 and 0.02 μg/mL and quantification limits of 0.03 and 0.05 μg/mL, respectively. The high sensitivity of the developed method permitted the simultaneous determination of both drugs in spiked plasma samples with % recoveries ranging from 95.0 to 103.25 with acceptable standard deviation values of 1.92 and 3.04 for remdesivir and simeprevir, respectively. The validation of the approach was approved by the International Council of Harmonization (ICH) guidelines.

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Application of different spectrofluorimetric methods for determination of lesinurad and allopurinol in pharmaceutical preparation and human plasma

Cited by 18 publications

References 11 publications

Quantitative analysis of favipiravir and hydroxychloroquine as FDA‐approved drugs for treatment of COVID‐19 using synchronous spectrofluorimetry: application to pharmaceutical formulations and biological fluids

Quantitative analysis of favipiravir and hydroxychloroquine as FDA‐approved drugs for treatment of COVID‐19 using synchronous spectrofluorimetry: application to pharmaceutical formulations and biological fluids

A Brief Review of Analytical Methods for the Estimation of Allopurinol in Pharmaceutical Formulation and Biological Matrices

Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma

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