Application of denaturing gradient gel electrophoresis for detection of bacterial and yeast communities along a salinity gradient in the estuary of the Cachoeira River in Brazil

Rodrigues, Cheryl; Souza, Samara Silva de; Rezende, Roberto; Silva, A.; Andrioli, J.L.; Costa, H.S.; Fontana, Renato; Dias, João Carlos Teixeira

doi:10.4238/2013.may.21.6

Cited by 7 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A steep reduction in salinity from the seawater to the tidal fresh zone is considered to have triggered the bacterial community shift in the marine tar balls. Our observation was consistent with previous literature that showed different microbial diversity along salinity gradients in estuaries [17, 18] and a study reporting unique hydrocarbon-enriched bacterial communities established in response to changed salinity levels [16]. However, the studies mentioned above used water samples, while our research tested marine tar balls collected from the Gulf of Mexico.…”

Section: Resultssupporting

confidence: 92%

Bacterial Community Shift in Marine Tar Balls following Low Salinity Exposure

Tran

Park

2017

Preprint

View full text Add to dashboard Cite

The persistence and recurrence of tar balls causes detrimental effects on coastal wildlife, tourism, and 7 fisheries. Previously, the human pathogen Vibrio vulnificus was detected in marine tar balls in the Gulf of Mexico. 8Marine tar balls also migrate to low-salt water bodies near the shore. The bacterial communities in tar balls from these 9 environments, however, have not been fully characterized. Herein we describe our studies on the effect of reduced 10 salinity on bacterial communities in marine tar balls. Tar balls collected from the Gulf of Mexico were incubated in 11 deionized water for six months, and their microbial fingerprints were visualized using denaturing gradient gel 12 electrophoresis. Our data show that the indigenous bacterial communities in marine tar balls shifted after being 13 exposed to low-salinity condition. Dominant genera in the tar balls at low salinity were Aquisalimonas and 14 Parvibaculum, neither of which includes known human pathogens. 15

show abstract

Section: Resultssupporting

confidence: 92%

Bacterial Community Shift in Marine Tar Balls following Low Salinity Exposure

Tran

Park

2017

Preprint

View full text Add to dashboard Cite

show abstract

“…These extracts also exhibited antimicrobial, anti-inflammatory (3)(4)(5). These ecosystems also provide food and shelter to a number of organisms maintaining the biodiversity (6). To reiterate, these ecosystems are ultimate sink of several man-made pollutants from industrialization, modern life styles, agricultural run-offs and municipal sewage systems (7,8).…”

Section: Introductionmentioning

confidence: 99%

Abiotic stress tolerance in mangroves with a special reference to salinity

Acharya

Pradhan²,

Mahalik³

et al. 2023

Plant Sci. Today

View full text Add to dashboard Cite

Since mangroves are found near extremely transitional ecosystems, they face a lot of physico-chemical perturbations. As mangroves possess a unique ecotone, they experience many abiotic stressors viz. salinity, metal, oil, humidity temperature, nutrient and a wide range of biotic interactions. Amongst all, salinity is the most important factor affecting mangrove physiology and biochemistry, and thereby regulating the organic matter contribution to the consumers underneath. Exploitation by human, being a dominant biotic interference, is above the rate at which natural replacement of mangrove vegetation occur. Mal-nutrition is a limiting factor in growth and reproduction of many mangroves whereas nutrient replenishment reduces the phytotoxicity of heavy metals. Different environmental pollutants including heavy metals, recalcitrant, cosmetics, petroleum oil and endocrine disrupters have reported impact on various mangroves and associated biota. Stress tolerance in mangroves involves various mechanism including morphological and anatomical features, osmoregulation, water use efficiency, salt secretion, salt exclusion and salt accumulation and molecular regulations. Various aspects of salt tolerance strategies of mangroves related to their growth, biochemical anatomy and physiology were reported by many researchers.

show abstract

“…Moreover, the high temperature and pressure generated by the explosion interact with the low temperature, high bacterial content, high osmotic pressure and high alkalinity of the sea water, thus resulting in a wound with infection in the surrounding tissues and other conditions including microcirculation disorders. It also causes local oedema, haemorrhage, degeneration and other symptoms, with the severe cases exhibiting tissue necrosis and systemic complications .…”

Section: Introductionmentioning

confidence: 99%

Application of vacuum sealing drainage to the treatment of seawater‐immersed blast‐injury wounds

Shi

Sun

Cao

et al. 2015

International Wound Journal

View full text Add to dashboard Cite

The aims of this study were to observe the effects of vacuum sealing drainage (VSD) with three different negative pressures on the wound healing rate, macrophage count and the expression of hyaluronic acid (HA) as well as its receptor CD44 in seawater-immersed blast-injury wounds (SIBIW) and to determine the optimal negative pressure value. In a minipig SIBIW model, different suction pressures and routine dressing were applied. Histological and immunohistochemical comparisons as well as molecular biology methods were performed to compare the wound healing conditions, macrophage count and the levels of HA and CD44. The wound healing rate of the VSD group was significantly higher than that of the control group, with the -120 mmHg group exhibiting the best effects. The macrophage count of the VSD group was higher than that of the control group. The HA level fluctuation was higher in the VSD group, with the -120 mmHg and the -180 mmHg groups showing the most significant fluctuation (P < 0·05). CD44 was expressed in the full-thickness wound-limbic tissues and was higher in the treatment group than that in the control group, with the -120 mmHg group having the most obvious expression. VSD significantly improved the healing ability and increased the macrophage count and the HA content. It also promoted CD44 expression. -120 mmHg is the optimal negative pressure value.

show abstract

Application of denaturing gradient gel electrophoresis for detection of bacterial and yeast communities along a salinity gradient in the estuary of the Cachoeira River in Brazil

Cited by 7 publications

References 15 publications

Bacterial Community Shift in Marine Tar Balls following Low Salinity Exposure

Bacterial Community Shift in Marine Tar Balls following Low Salinity Exposure

Abiotic stress tolerance in mangroves with a special reference to salinity

Application of vacuum sealing drainage to the treatment of seawater‐immersed blast‐injury wounds

Contact Info

Product

Resources

About