Application of Data-Independent Acquisition Approach to Study the Proteome Change from Early to Later Phases of Tomato Pathogenesis Responses

Abstract

Plants and pathogens are entangled in a continual arms race. Plants have evolved dynamic defence and immune mechanisms to resist infection and enhance immunity for second wave attacks from the same or different types of pathogenic species. In addition to evolutionarily and physiological changes, plant-pathogen interaction is also highly dynamic at the molecular level. Recently, an emerging quantitative mass spectrometry-based proteomics approach named data-independent acquisition (DIA), has been developed for the analysis of the proteome in a high-throughput fashion. In this study, the DIA approach was applied to quantitatively trace the change in the plant proteome from the early to the later stage of pathogenesis progression. This study revealed that at the early stage of the pathogenesis response, proteins directly related to the chaperon were regulated for the defence proteins. At the later stage, not only the defence proteins but also a set of the pathogen-associated molecular pattern-triggered immunity (PTI) and effector triggered immunity (ETI)-related proteins were highly induced. Our findings show the dynamics of the plant regulation of pathogenesis at the protein level and demonstrate the potential of using the DIA approach for tracing the dynamics of the plant proteome during pathogenesis responses.

Protein Elicitor PeBL1 of Brevibacillus laterosporus Enhances Resistance Against Myzus persicae in Tomato

“…AIMI and LOX code for fatty acid beta-oxidation multifunctional proteins. Lipoxygenase up regulation in JA occurs upon Pseudomonas inoculation in tomato plants [59]. KAT2 codes A 3-ketoacyl-CoA thiolase, carrying out wound-activated responses, as shown in the biosynthesis of JA in wounded Arabidopsis plants [60].…”

Biocontrol Potential of Purified Elicitor Protein PeBL1 Extracted from Brevibacillus laterosporus Strain A60 and Its Capacity in the Induction of Defense Process against Cucumber Aphid (Myzus persicae) in Cucumber (Cucumis sativus)

“…PAL1 coding for ammonia-lyase phenylalanine is involved in cell wall construction, as demonstrated in Arabidopsis [ 59 ]. JA and LOX1 up-regulation occur in cucumber plant Pseudomonas injection [ 59 , 60 ]. PR1 and PR2 are systemically acquired (SAR) pathogenesis-related proteins [ 13 , 61 , 62 ].…”

Quantitative Proteomics Reveals the Dynamic Regulation of the Tomato Proteome in Response to Phytophthora infestans

“…The sample was then homogenized with 0.9 mL of ice-cold pH 8.0 homogenization buffer (0. For each sample, 100 µg of the solubilized tomato total protein was reduced, alkylated and proteolyzed with lysyl endopeptidase and trypsin following the steps previously described [81]. The digested sample was then desalted using the 100-mg tC18 SepPak cartridge (Waters Corporation, Milford, MA, USA).…”