Quantitative Proteomics Reveals the Dynamic Regulation of the Tomato Proteome in Response to Phytophthora infestans

Abstract

Late blight (LB) disease is a major threat to potato and tomato production. It is caused by the hemibiotrophic pathogen, Phytophthora infestans. P. infestans can destroy all of the major organs in plants of susceptible crops and result in a total loss of productivity. At the early pathogenesis stage, this hemibiotrophic oomycete pathogen causes an asymptomatic biotrophic infection in hosts, which then progresses to a necrotrophic phase at the later infection stage. In this study, to examine how the tomato proteome is regulated by P. infestans at different stages of pathogenesis, a data-independent acquisition (DIA) proteomics approach was used to trace the dynamics of the protein regulation. A comprehensive picture of the regulation of tomato proteins functioning in the immunity, signaling, defense, and metabolism pathways at different stages of P. infestans infection is revealed. Among the regulated proteins, several involved in mediating plant defense responses were found to be differentially regulated at the transcriptional or translational levels across different pathogenesis phases. This study increases understanding of the pathogenesis of P. infestans in tomato and also identifies key transcriptional and translational events possibly targeted by the pathogen during different phases of its life cycle, thus providing novel insights for developing a new strategy towards better control of LB disease in tomato.

Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space

“…Within MF also ‘beta-glucosidase’ and ‘glucosidase activity’ terms were represented with four upregulated DEGs. Among them, Solyc01g059965.1.1 has been demonstrated to be implicated in the defense of tomato plants against pathogens ( Murata et al, 2019 ), and its expression has been found to be modulated in tomatoes upon infection with Cladosporium fulvum and Phytophthora infestans ( van Kan et al, 1992 ; Fan et al, 2021 ). Upregulation of Solyc06g073760.3.1 has been correlated to increased softening of transgenic tomato overexpressing a MADS-box transcription factor, affecting fruit development and ripening ( Huang et al, 2017 ).…”

Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato

“…One pathogenesis-related (PR)-10 family protein salt tolerance homolog 2 (STH-2) was significantly induced at both transcription and protein levels during both PTI and ETI ( Figure 4C ). It was reported to be increased in tomato leaves in response to Phytophthora infestans ( Fan et al, 2021 ). Interestingly, phosphorylation of a conserved immunity regulator RPM1-interacting protein 4 (RIN4) ( Zhao et al, 2021 ) was significantly increased (e.g., Ser-144) during both PTI and ETI ( Supplementary Table 3 ).…”

Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions

“…The significantly changed proteins belonged to immune response, redox processes, energy generation, and carbon fixation in the chloroplast. Fan et al [ 100 ] employed a DIA-MS method to study the interaction between tomato and the hemibiotrophic oomycete pathogen Phytophthora infestans . Among the changed proteins, several were involved in plant defense responses, metabolic pathways, and signaling.…”