“…Third, particles can be used to amplify detection signals on PADs, thereby eliminating the need for enzymatic amplification. Over the past few years, there have been many reports of the use of nanoparticles for colorimetric (1, 33,35,39,63,69,74,75,79,81,84,[139][140][141] and electrochemical (4-7, 10, 35, 51, 61, 62, 102, 142, 143) detection on both 2D and 3D PADs. Nanotubes, -rods, or -wires comprised of carbon (60, 61, 87-89, 91, 102, 111, 113), zinc oxide (96), gold (86,142), platinum (144), nickel (144), or copper (144) have been integrated into paper-based sensors to enhance detection.…”