Application of core–shell PEGylated CdS/Cd(OH)2 quantum dots as biolabels of Trypanosoma cruzi parasites

Chaves, Claudilene R.; Fontes, Adriana; Farias, Patrícia M. A.; Santos, Beate S.; Menezes, Frederico Duarte de; Ferreira, Ricardo C.; César, Carlos L.; Galembeck, André; Figueiredo, Regina Célia Bressan Queiroz de

doi:10.1016/j.apsusc.2008.07.035

Cited by 15 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such particles can bind to cell surface nonspecifically via electrostatic interaction or specifically if equipped with receptors ligands (Parak et al 2005). Chaves et al (2008) showed that QDs enter in the T. cruzi cells by endocytosis to the cytostome and are delivered directly to the reservosomes. Results of our group demonstrated that the uptake of quantum dots bioconjugated with lectin (Sambucus nigra agglutinin; Fig.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

et al. 2009

View full text Add to dashboard Cite

Semiconductor quantum dots (QDs) are highly fluorescent nanocrystals markers that allow long photobleaching and do not destroy the parasites. In this paper, we used fluorescent core shell quantum dots to perform studies of live parasite-vector interaction processes without any observable effect on the vitality of parasites. These nanocrystals were synthesized in aqueous medium and physiological pH, which is very important for monitoring live cells activities, and conjugated with molecules such as lectins to label specific carbohydrates involved on the parasite-vector interaction. These QDs were successfully used for the study of in vitro and in vivo interaction of Trypanosoma cruzi and the triatomine Rhodnius prolixus. These QDs allowed us to acquire real time confocal images sequences of live T. cruzi-R. prolixus interactions for an extended period, causing no damage to the cells. By zooming to the region of interest, we have been able to acquire confocal images at the three to four frames per second rate. Our results show that QDs are physiological fluorescent markers capable to label living parasites and insect vector cells. QDs can be functionalized with lectins to specifically mark surface carbohydrates on perimicrovillar membrane of R. prolixus to follow, visualize, and understand interaction between vectors and its parasites in real-time.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Under proper conditions, it is possible to distinguish between serum positive and negative to Chagas disease. [87,88]). a Nucleus, b flagellar pocket, c flagellum, d cytostome, e cytoplasmic vesicles, f cytoskeleton Joining the last two methods (as an "immunotechnique" is applied, but gold acts as a supporting electrode for sensing purposes, instead of labeling), Ferreira et al [27] immobilized Chagas disease antigens in a gold surface, and the anti-T. cruzi antibodies present in the serum sample were captured by the antigen, remaining connected to the solid phase.…”

Section: Detection Methods Using Goldmentioning

confidence: 99%

The role of nanogold in human tropical diseases: research, detection and therapy

Almeida

Carabineiro

2013

Gold Bull

View full text Add to dashboard Cite

“…Pegylation, however, is not universally inert. We have demonstrated that PEGcoated CdS/Cd(OH)2 QDs are able to bind live parasites cells, triggering endocytosis by specialized endocytic pathways such as those using the flagellar pocket (Chaves et al, 2008).…”

Section: Quantum Dots In Biomedical Research 285mentioning

confidence: 99%

Quantum Dots in Biomedical Research

Fontes¹,

Lira²,

Seabra³

et al. 2012

Biomedical Engineering - Technical Applications in Medicine

View full text Add to dashboard Cite

Application of core–shell PEGylated CdS/Cd(OH)2 quantum dots as biolabels of Trypanosoma cruzi parasites

Cited by 15 publications

References 8 publications

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

The role of nanogold in human tropical diseases: research, detection and therapy

Quantum Dots in Biomedical Research

Contact Info

Product

Resources

About