Application of cell-based bioassays to evaluate treatment efficacy of conventional and intensified treatment wetlands

Nivala, Jaime; Neale, Peta A.; Haasis, Tobias; Kahl, Stefanie; König, Maria; Müller, Roland; Reemtsma, Thorsten; Schlichting, Rita; Escher, Beate I.

doi:10.1039/c7ew00341b

Cited by 24 publications

(61 citation statements)

References 57 publications

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“…Herein, we reported significant PPARγ activity at 0.001× of the CO UOG wastewater sample, and at 0.04× in one UOG-impacted water sample, with relative potencies ranging from 0.00025 to 0.005× (EC 10 , Table S2), considerably more potent than those reported in other studies. Other wastewater-impacted samples exhibited relative potencies (EC 10 ) of 0.25–0.45, similar to or lower than those reported in these other studies (Nivala et al, 2018; Konig et al, 2017). Given the small samples sizes in all of these studies, more comprehensive assessment of water for PPARγ and adipogenic activity is needed.…”

Section: Discussionsupporting

confidence: 82%

“…Few studies have assessed PPARγ activity of water samples, though recent work reported significant PPARγ activity at concentrations as low as 0.025× relative water concentrations for oil sands process-affected water (Peng et al, 2016), and significant triglyceride accumulation by 1× fractions. Other studies have recently reported PPARγ activity of water samples impacted by wastewater treatment plants, reporting relative potencies (EC 10 ) of 0.2–5.7× (Nivala et al, 2018) and 8–215 relative enrichment factors (Konig et al, 2017). Herein, we reported significant PPARγ activity at 0.001× of the CO UOG wastewater sample, and at 0.04× in one UOG-impacted water sample, with relative potencies ranging from 0.00025 to 0.005× (EC 10 , Table S2), considerably more potent than those reported in other studies.…”

Section: Discussionmentioning

confidence: 99%

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Unconventional oil and gas chemicals and wastewater-impacted water samples promote adipogenesis via PPARγ-dependent and independent mechanisms in 3T3-L1 cells

Kassotis

Nagel

Stapleton

2018

Science of The Total Environment

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Unconventional oil and natural gas (UOG) operations have contributed to a surge in domestic oil and natural gas production in the United States, combining horizontal drilling with hydraulic fracturing to unlock previously inaccessible fossil fuel deposits. >1000 organic chemicals are used in the production process, and wastewater is produced following injection and for the life of the producing well. This wastewater is typically disposed of via injecting into disposal wells for long-term storage, treatment and discharge from wastewater treatment plants, and/or storage in open evaporation pits; however, wastewater spill rates are reported at 2–20% of active well sites across regions, increasing concerns about the environmental impacts of these wastewaters. This study assessed adipogenic activity (both triglyceride accumulation and pre-adipocyte proliferation) for a mixture of 23 commonly used UOG chemicals and a small subset of UOG wastewater-impacted surface water extracts from Colorado and West Virginia, using 3T3–L1 cells and a peroxisome proliferator activated receptor gamma (PPARγ) reporter assay. We report potent and efficacious adipogenic activity induced by both a laboratory-created UOG chemical mixture and UOG-impacted water samples at concentrations below environmental levels. We further report activation of PPARγ at similar concentrations for some samples, suggesting a causative molecular pathway for the observed effects, but not for other adipogenic samples, implicating PPARγ-dependent and independent effects from UOG associated chemicals. Taken together, these results suggest that UOG wastewater has the potential to impact metabolic health at environmentally relevant concentrations.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Unconventional oil and gas chemicals and wastewater-impacted water samples promote adipogenesis via PPARγ-dependent and independent mechanisms in 3T3-L1 cells

Kassotis

Nagel

Stapleton

2018

Science of The Total Environment

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“…Cell-Based Bioassays. To measure the activation of xenobiotic metabolism signaling pathways, the AhR-CALUX assay described by Brennan et al (2015) 27 and performed according to Nivala et al (2018) 34 and the PPARγ-bla GeneBLAzer assay 28,29 following the method by Neale et al…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…28 Directly before dosing and after 24 h of exposure, the confluency of the cells in all wells in the cell plates was measured using an IncuCyte S3 live cell imaging system (Essen BioScience, Ann Arbor, Michigan, U.S.A.). 34 After 24 h the reporter gene product was quantified after adding the appropriate substrates and measuring fluorescence or luminescence using a microplate reader (Infinite M1000 Pro, Tecan, Grodig/Salzburg, Germany). A first highconcentration dosing (of relative enrichment factors of up to 167 of the extracts, see "Data Evaluation") combined with serial dilution was performed for the detection of cytotoxicity and for range finding.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Effects of Leachates from UV-Weathered Microplastic in Cell-Based Bioassays

Rummel

Escher

Sandblom

et al. 2019

Environ. Sci. Technol.

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103

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Standard ecotoxicological testing of microplastic does not provide insight into the influence that environmental weathering by, e.g., UV light has on related effects. In this study, we leached chemicals from plastic into artificial seawater during simulated UV-induced weathering. We tested largely additive-free preproduction polyethylene, polyethylene terephthalate, polypropylene, and polystyrene and two types of plastic obtained from electronic equipment as positive controls. Leachates were concentrated by solid-phase extraction and dosed into cell-based bioassays that cover (i) cytotoxicity; (ii) activation of metabolic enzymes via binding to the arylhydrocarbon receptor (AhR) and the peroxisome proliferator-activated receptor (PPARγ); (iii) specific, receptor-mediated effects (estrogenicity, ERα); and (iv) adaptive response to oxidative stress (AREc32). LC-HRMS analysis was used to identify possible chain-scission products of polymer degradation, which were then tested in AREc32 and PPARγ. Explicit activation of all assays by the positive controls provided proof-of-concept of the experimental setup to demonstrate effects of chemicals liberated during weathering. All plastic leachates activated the oxidative stress response, in most cases with increased induction by UV-treated samples compared to dark controls. For PPARγ, polyethylene-specific effects were partially explained by the detected dicarboxylic acids. Since the preproduction plastic showed low effects often in the range of the blanks future studies should investigate implications of weathering on end consumer products containing additives.

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“…In another study, aeration has been shown to improve removal of ibuprofen in planted and unplanted wetland mesocosms treating synthetic wastewater [95]. The first application of in vitro bioassays to treatment wetlands found that aerated wetlands were able to remove biological activity to a greater extent than a conventional municipal treatment plant [96].…”

Section: Research Trendsmentioning

confidence: 99%

Recent Advances in the Application, Design, and Operations & Maintenance of Aerated Treatment Wetlands

Nivala

Murphy²,

Freeman³

2020

Water

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This paper outlines recent advances in the design, application, and operations and maintenance (O&M) of aerated treatment wetland systems as well as current research trends. We provide the first-ever comprehensive estimate of the number and geographical distribution of aerated treatment wetlands worldwide and review new developments in aerated wetland design and application. This paper also presents and discusses first-hand experiences and challenges with the O&M of full-scale aerated treatment wetland systems, which is an important aspect that is currently not well reported in the literature. Knowledge gaps and suggestions for future research on aerated treatment wetlands are provided.

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Application of cell-based bioassays to evaluate treatment efficacy of conventional and intensified treatment wetlands

Abstract: Removal efficacy of treatment wetlands was assessed using a comprehensive bioassay test battery, with intensified wetlands found to remove 74–100% of biological activity.

Cited by 24 publications

References 57 publications

Unconventional oil and gas chemicals and wastewater-impacted water samples promote adipogenesis via PPARγ-dependent and independent mechanisms in 3T3-L1 cells

Unconventional oil and gas chemicals and wastewater-impacted water samples promote adipogenesis via PPARγ-dependent and independent mechanisms in 3T3-L1 cells

Effects of Leachates from UV-Weathered Microplastic in Cell-Based Bioassays

Recent Advances in the Application, Design, and Operations & Maintenance of Aerated Treatment Wetlands

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