Application of Carbon Nanotubes for Plant Genetic Transformation

Burlaka, O. M.; Pirko, Ya. V.; Yemets, А. І.; Blume, Ya. B.

doi:10.1007/978-3-319-06611-0_20

Cited by 7 publications

(7 citation statements)

References 193 publications

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“…These methods are better efficacy and have the stable integration as well as the rapid expression for the transgenic process using nanoparticles (Rai et al 2015;Khodakovskaya and Lahiani 2016;Shukla et al 2016;Pacheco and Buzea 2017). These nanoparticles could be conjugated with nucleic acid developing the methodology of gene transfer in plants and improving the efficacy, stability and accuracy of transgenic process making it less time-consuming (Burlaka et al 2015;Rai et al 2015;Elahian et al 2017). Therefore, it could be concluded that, the encapsulation of nanomaterials could be considered the best method in delivering different agro-chemicals or different agricultural active ingredients in more efficient manner.…”

Section: Nanocapsules For Efficient Delivery Agrochemicalsmentioning

confidence: 99%

Nanobiotechnology for Plants

Abdalla¹,

Ragab

Fári

et al. 2019

EBSS

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N ANOTECHNOLOGY has been revolutionized penetrating all sectors in our life through the nanoscience as an essential science for a wide range of technologies. Amazing achievements resulted from this nanotechnology including all agricultural fields such as plant nutrition and crop productivity, energy sector, food sector, and plant biotechnology. A conjugation between plant biotechnology and nanotechnology has been produced an important science called plant bio-nanotechnology. Several fields have been invaded through different nanobiotechnology applications in agriculture including (1) the nanotechnology of encapsulated agro-chemicals, (2) the monitoring of different environmental stresses and crop conditions using nanobiosensors, (3) the improvement of crop production and ameliorating plants against diseases and (4) solution several environmental problems. The crop productivity also could be improved using some new agro-chemicals (e.g., nanofertilizers and nanopesticides). These agro-chemicals are very effective in delivering encapsulating nanomaterials and then enhancement the productivity of crops as well as the suppress plant pests and diseases and protecting the environment from pollution. On the other hand, nanoparticles could enter the food chain via different nano-agrochemicals or nano-processed foods. Therefore, many approaches including uptake of nanoparticles by plants, entry and bio-distribution of nanoparticles into the food chain are needed before using of different bionanotechnological tools in agro-production sector. Further new regulations should be created or rebuilt for new approaches in plant bionanotechnology. Therefore, this review will focus on our needs and risks in the plant nanobiotechnology.

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Section: Nanocapsules For Efficient Delivery Agrochemicalsmentioning

confidence: 99%

Nanobiotechnology for Plants

Abdalla¹,

Ragab

Fári

et al. 2019

EBSS

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“…Diameter of SWCNTs is less than 5 nm that allows them to pass through pores of a plant's cell wall (Burlaka et al, 2015).…”

Section: Swcnt and Mwcntmentioning

confidence: 99%

“…This is also understood by a simulation using Microscope Simulator program (Campbell et al, 2008). Burlaka et al (2015) claims that the structural change in DNA during the formation of conjugation is due to A-B conformation transition and compared the DNA-CNT interaction as the one in chromosomes during DNA assembly by histones. There was a decrease in the water molecules of the DNA observed, which suggested that the nanotubes displace these water molecules during their interaction with DNA.…”

Section: Dna Binding To Cnts and Gene Deliverymentioning

confidence: 99%

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Role of carbon nanotubes (CNTs) in transgenic plant development

Pawar,

Anumalla,

Sharma

2023

Biotech & Bioengineering

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Carbon nanotubes (CNTs) are nanostructures, allotropes of carbon which are made up of graphene sheets wrapped around it forming cylindrical structures. CNTs have been regarded to have interesting and attractive physical and chemical properties and have been tremendously used in genetic engineering. Understanding the role of CNTs in development of transgenic plants, review of research papers in the field was done. CNTs are classified into two categories: the single‐walled and multiwalled (MWCNTs) structures. They are valuable vectors in various biomedicine fields such as Gene delivery, Drug delivery, Immunotherapy, Tissue engineering, and Biomedical imaging and also, they deliver the DNA without damaging the cells. Based on recent studies, the functionalization of CNTs when combined with some other suitable molecules can drastically subside their toxic effects. Having unique properties such as small size, larger surface area is useful in delivering DNA into mammalian cells as well. Modifications in CNTs can make nucleic acids adhere to them even more efficiently. Also, MWCNTs are crucial in delivery DNA into the cytoplasm. Based on other methods, the CNTs‐DNA are a preferred choice and the inclination toward double‐stranded DNA is used over single‐stranded DNA in gene delivery shows effective results. The only downside of CNTs is that they are hydrophobic and are difficult to form an aqueous solution, thus limiting their applicability. This review will aid you in comprehending useful knowledge related to a general overview of topics related to CNTs.

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“…Eruca sativa (arugula), Triticum aestivum (wheat), and Gossypium hirsutum (cotton) (Demirer et al 2019). The loaded CNT can enter through the plant cell wall and deliver targeted molecules into the plant cells with low cytotoxicity (Burlaka et al 2014). Using CNT to deliver plasmid DNA into plant cells provides advantages over the conventional transformation methods with increasing efficiency, applicability, and reproducibility (Ohadi Rafsanjani et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Carbon-nanotube for Transient Expression in Rice Calli

et al. 2022

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Transient gene expression is an important technique in gene functional analysis, protein production and in plants. However, traditional transient expression methods using Agrobacterium are time-consuming with low efficiency. In this study, we demonstrated the use of a single-walled carbon nanotube (SWCNT) to deliver 35S:mCherry:pCXSN plasmid into rice calli. This transient expression protocol used a plastic medical syringe to create the physical pressure to help the delivery of plasmid DNA into plant cells. This protocol is relatively easy to perform and low cost. The transient expression was observed under fluorescence microscopy, and the mCherry fluorescence signal was quantified. The plasmid DNA was delivered into the rice cell using a 3:1 ratio (plasmid: carbon nanotube). The result showed that the mCherry signal of carbon nanotube + plasmid DNA treatment was the highest signal at 3 days post-transformation and decreased to a lower signal at 6 days post-transformation. Moreover, the quantitative analysis of mCherry mean intensity revealed that the signal intensity of carbon nanotube + plasmid DNA treatment was the highest level, and significantly higher than the control treatments at 3 days post-transformation. Plasmid DNA can be transported easily into plant calli using this carbon nanotube transient expression protocol.

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Application of Carbon Nanotubes for Plant Genetic Transformation

Cited by 7 publications

References 193 publications

Nanobiotechnology for Plants

Nanobiotechnology for Plants

Role of carbon nanotubes (CNTs) in transgenic plant development

Carbon-nanotube for Transient Expression in Rice Calli

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